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(    & %"        *    ;#%"      ++)3100                              d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}|zxwtspomligecb`]OOOGGGddd{{{   $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 26d92534ce2a4cc79d8e4e9cdb0a7e6dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-Well"K1317_F1_Thunbergia_fragans_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z%DD$>@e=)$N!ATg=# IuI+ y> Ve @_o5'lx-n0 1\wrQ%5Q[P7>qK9c /J[b_ZZgv+z7 0CNPMKRd{j\a|H=lX,    /?D=-$4=:,  &GcohQ6%&8QdgX<&?T^[M</)*+)#(;MWS?$ 2=@AIf~ipS= xI 3cd71JQB%$IgrhP6%!)4;9,.OfiW9'#     5CE9& /AHA0   /3+"7JRK7 %CZbYB%  $6>9)1BF<)  4cf7!+00)$36, 0GSM8 ^AD(f*[O   (Nv`7BctmS37YrrV*CzX& F1_a}/5[zuW?Co&Bd a{#m JK1IBA@bw]e <kAH      EW|3. ,=IKC2  %&   ='q^d''3;FYtl;-=A:22>Rfoj[I<9=A?5$  %,1.& "!",.'  *7;4#,/'$]5*X. :o]}e2|]#j +9;4.8^ 6})Z!a- UGi# z  (593% ! 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I    +P.20X#+|I H=     #+,7 4 $!M= \6  1 1<\?  gF~T99 Ti8,4xN74pZP8,K9g"U 4 647 7ps\wYRHi`y?L4R?5iUaew38&pGd P3uMpj$Pt#E?,F9"BN:/+s,)UFN(*!fG)EM^M(WAup9<CQ=k +]!GD WFv#K5(1(,i\KK]Wn>,:jpE;bB,jY}|c'#h*mIaPF`W7D4 NRB8yH5gTVv#:pol)/>IPg+I) i#mKD%f `'d.,dd N8kdQ8@/ymZm)j5gvr9D/=xX|geycvQ=XxB{s-c3)9<!{ (/:`dF(;H*f #bEY}qeZi{1TSpZ_NvCr<7r^sFXBLtx_:V(kByVr-SwWDxqXqEEzWFp8Wi &KFH;b`P~4 K+,=o* w]U~y<wEGbup PLgxgg!)]g_>@Esh`| zt `EC3VGj|Ib<o*uw? `{`PGY'f~/8)^Uc {K?+aph?GAATCCATGAGCGATCGGGCCCTGCAGTGGAGCCACTCAAACAAACAGAAAATATGAGATGCACGCAAAAAAAGCCTAAGGCCTACATTAAGTACCCACAATCACCCGGCCAATACAAAGGCATCGTGGAATGAAGATCCTTCCCATATACATTCCTCCGAAGGCATTCGCAACACCACCTTAGCTGCATCAAGCGGCACATGTTACCCCCTTAGTTCCGGGCCCATGCCCTGATCCACCACGACAAGGACAAACGTCACAACCCCACAAGTTACCATGGTACGAACCCTGCTTCGCAAAACATAACTACATTCTCCAATTCCTACATCAAGTCCACACTCCACCACCTCTGCCTGAAACTACAAAAGCCACAGCTATTTAAGCCCACCCACCGAACACGATACCTACCACAGAACTCGGAGCCCCCGGGCGGGGACCACAACACCACCCAAGGGGGTAGGCGCCCGGTCAAAAACACAAATAATCGGATACAGTTTGGTGCCTCGTAAAAAAGCGACTTCGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:37:07 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTSRBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :u`TDATA :u`&DATA :u`?DATA :u`DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~QeLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS*NP1AM >P1RL :P1WD CP2AM KAP2BA  OYP2RL G)PBAS  ,PBAS  *PCON  8PCON  6PDMFTPDMF*ePLOC 2PLOC .PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%*FSCAN@SMED SMLtSMPL##TSPACAi SPAC*^SPACAi SVER4.0SVER RSVER,,/Scal@Scan@TUBEF1Tmpr<UserphARphCH*|phDYbigphQLcphTRphTR,This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTSRBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :u`TDATA :u`&DATA :u`?DATA :u`DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabaIFVoca8FWO_GATCFeat//a GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~QeLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS*NP1AM >P1RL :P1WD CP2AM KAP2BA  OYP2RL G)PBAS  ,PBAS  *PCON  8PCON  6PDMFTPDMF*ePLOC 2PLOC .PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%*FSCAN@SMED SMLtSMPL##TSPACAi SPAC*^SPACAi SVER4.0SVER RSVER,,/Scal@Scan@TUBEF1Tmpr<UserphARphCH*|phDYbigphQLcphTRphTRU[lWQXb^kOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gt]8u9i$%KP$cKOvx30d,sk%O[c7(/Pi%Q?&dT.+6P-2ZE<}qjM>Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*Pv>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`]vWs*D_rj|YnG|urG&/o{o;Mj2Q&?R2gcV]j4 VI6DZrm!m4>xx$cD\<]*:8539l@A_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_P1RL :P1WD CP2AM KAP2BA  OYP2RL G)PBAS  ,PBAS  *PCON  8PCON  6PDMFTPDMF*ePLOC 2PLOC .PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%*FSCAN@SMED SMLtSMPL##TSPACAi SPAC*^SPACAi SVER4.0SVER RSVER,,/Scal@Scan@TUBEF1Tmpr<UserphARphCH*|phDYbigphQLcphTRphTR