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"9d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}|zxwtspomligecb`]OOOGGGddd{{{   $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6ae727a4817840c082034cd40fb20f3fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-WellK1317_E7_Asplenium_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z#C^v-F^uCv  >kFR<M309@<*o6oR5rS+psqmz{lhn~ Cis\@!ylaYTQQSVXUM@0"#0=FID8'  7MYWG0  #%#.?F>+&& %-22.'  -<IT]aca^]`iuscUNOV^a\P@3.4AQ]a\QC8213579;==;4+$!&0:=7)  #,023697/! %"  (>j!Q^46o{."[q3S x^M?1#   5WFysS/kQ=0' &3?FG@3# !:Y<Q\]WMGLfg0"^7    )8AC@:4/,(!     )VwOmIlX]:H45;;2" &,/-'    1s@ek'l yJ+            *<HKE7%Lf55D H9= d oVth!b.lNA>BJRZ^^XM?-0c$F5\=QU-$.?PZ]ZTONPSURJ=.!%+-+&!   ,@MPF5     &.1-% DrktDYB5Z|celnf[SORVXVM@0  '=SdmokbVJ>3*%$&*-+$'*% (054,   "'&!/9;93-(%"      %+46"-z-@KRTSLC920132,"  #%  #2;;3' />HNPRSVUN@, -77- 8*RJ*/|  *7CGA2$(Kr&4|"'/g}pPCWS8l@~T:o pw X7 !/69=ACB><>FQ\a^VJ@70(   %$1! s~ij5 '&!!"  _p]Y#6CMV`hkdR:!   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    !*" #     )    * %6 % . 4& +% &+ 8%++ /-5)!$8*2/@,")! ---%0$9/." $$)0-7*BY($$E-,).&/?$9968 *-<5g9926#:E.@$-EJ-X-F-8=75-- Z$--$--$9$08uH:P,R0=pp|pzF8 #R+yzn k iSbpAP&i%88wR:dWp7.\+Yd|=c^D|exFgW^Q] m2s:McdpTc!3V48 ,#!$p71l,2MV5;p ?pP!Hi+Io:RzCPOySoEyp(.=Wo=bqb s 3GJ <Ot2Cpq)3$Pdl^Hp81Z52pv5"Mkrh=rIQ78/u H D i5:\%nOwMubzp>8ob&?-WKflt$edF<(?Oje:KwU!X9H$, )-.@J?D,>a[] l1091LBF(I3wo>M][2?FE6<U)b0!-FA\:nJ)&X03L#1E:gITDWUsHB$W?AzI.8<DiZ>D6L?7"AE<c=r`p3:;ygEkKSOT6?1L\1>1=(;L5.6)NE8SW0C&-v#8P-tw]l3('X^_^@cK2J9,O[5xHm2BX<{9t5(7<*bkhA\Va^'NNe7*oD+,O9y3l<T'FJ?Nfd12U~hQ>`PPQ>?yS`D;!@UN]ZrETatY>@S{EEG*:?<hQ2LIp`qT\UHXhc^c*3Z;66&S`==0WJj/5`59>:%4bnEg?;X[Hc7aA%[aAnW2R3WeL\!BN3sX@#6dD76OFIy!t;'CAI,?XX0u9CA6D1&uQaCe3NUEHzf-QToVy_CKNlTI/ _{o9">qe l@)TGAAGAAAAAACCCTTCTTAAACCATTTACTCCAAACATATAAGTCTCAACTTCGGATCAAAACACATAACCATGAAACGCGGACGTAAGTGGACCACAGAGGACGAACCAACAGGCCGGCATGCCCCCGGACCAAGCCCGGCCTTCCGTTAGCTCCAAGGCCTCTCAAGGCATTCGGACCCCAAGGTCGGAAAGCGGTTTAATCCGAGAAGATTTCTTCCAATGGAGGTGTCTTCGTTATTTCCCCAGCGTCCTGTGTGAAGACCCAATGATTAACCATCGGAGCTATACACTTAAAATCCTCTTCAAGGAACAGCGCCACAAAGTTGCCTCCTTTCCCGGTAAGAACCAGGATTGGGTTTTCAGCCCTACTTGGCCTAATTTTTGGAAAAATTTAAGTATTCTGTGGTTAATGTTAAACTTGATAAGGAGGAAGGTATTTTAGGTGCAGTATTAATTATTGATTTTACCATATATAACGGTCCCTTAAGGTAGGATCCACTAAACCCCGAATTAGGCCATTATGCCCCACAGCATGGAAGTAGAGCTGGTTAACGCTTGTAGCCGGGAGTTGTGGTAACAACACCATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:37:51 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\DATA ?\SxDATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~~"LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR8P1AMMimP1RLMdP1WDMnP2AMMw;P2BAMM{P2RLMrPBASMMTPBASMMRkPCONMMbPCONMM`9PDMFTPDMFROPLOCM[PLOCMWPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R0SCAN@SMED SMLtSMPLTSPACAdSPACRHSPACAdSVER4.0SVER SVER,,/Scal@Scan@TUBEE7Tmpr<UserphARphCHRfphDYbigphQLcphTRphTR(5This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\DATA ?\SxDATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~~"LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR8P1AMMimP1RLMdP1WDMnP2AMMw;P2BAMM{P2RLMrPBASMMTPBASMMRkPCONMMbPCONMM`9PDMFTPDMFROPLOCM[PLOCMWPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R0SCAN@SMED SMLtSMPLTSPACAdSPACRHSPACAdSVER4.0SVER SVER,,/Scal@Scan@TUBEE7Tmpr<UserphARphCHRfphDYbigphQLcphTRphTR~WhC0-#JpPJwiO*$]_`#?Z9rS}G|u8 ]qU(6ji,x5?h8]Z~m$F5:U~2C 4"])|JjJDeV2E+qmj4I73wJzO`P{ e,&o.]/+M;`8hrjuO*@NzRoB+ks9?OkZKUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^M4'Uw\Pcc{\A_}$A>AR5Z(b|U2mC!KC_KdrWS[jWcgaWmUbe\W'KFkaqQltX[3>>B~:^gWiD!}%`8p3Tma4y# 9!6E.+#(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i6ti#O7(V6[/3-6)SE8pT/@MOsijd(DUfb9bn~!i3I0 .',`m~I$4Z)a>p$ybX%AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,HB*@Yy-4$&+VFl@i2+`'Mvf0iGo!P#`DDdUfI$.Ct+nA[Hh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob:``?(GIMfi83m ;op$SqwgE6FM;v&KT_ZO~|Qg\c9^Y"+wcAP8[&*CTC2Ow7fwYI$=9&4sKFMrRU)>`Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9}__';_/d@ikjF=mJGLbva].p>x'[IoOoh-gvxAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2AUDTB1Pt1B1Pt1BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\DATA ?\SxDATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~~"LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR8P1AMMimP1RLMdP1WDMnP2AMMw;P2BAMM{P2RLMrPBASMMTPBASMMRkPCONMMbPCONMM`9PDMFTPDMFROPLOCM[PLOCMWPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R0SCAN@SMED SMLtSMPLTSPACAdSPACRHSPACAdSVER4.0SVER SVER,,/Scal@Scan@TUBEE7Tmpr<UserphARphCHRfphDYbigphQLcphTRphTR