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  &=>A9KE7!!    ,3_JnM/lQPA'  :4TMwQvb=O:-%!     )''$ 2     d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}|zxwtspomligecb`]OOOGGGddd{{{   $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 39b767c780aa4d349f65f9a8f71ebc30Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-Well K1317_E5_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z$/5778=GR\dlus^J;45;CGC6"   ~~{ti^VUX_ehhfdcbceiq~ 8X}#GjHrW%U$}>u]ROPQLC5& "!")-+% """  #$$%,9Mbt~}scUNP\m}D9kStP0'=Wsv`F'ww}yeSD6*  *:Nl>l~`:lQ;*#.:HU`iqz+;B@8+W3      0U7lp=  'yg0Vv:    3y`j=D}?   %,27982' ,Q4R@   'Q@ rq  sh9t 84;C*S';}h!#*-+'"  3x\i QD`aC/! 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A -5!(a296 Uc[XC(7`F4^T GL'/G&Z+L`@/ 7=RWTc*!GTGd#&P0 c; <<?<;1 aG,=0&6 /J1QXBY` /(+N: ;, RND;BFS9B;()">^ST /;1 dL>NEAIG4"J` bLO"[2S,Q<"TF FOP\.>YK AI,X>"=^0VW $Qb^`KcP2[#T;2L\@&)Bb,[,]1LL7DXQ ?c6$ +"b`\,""LG09: I/I[EcT3LP,QEE ! L7G/RS^W;S5)1(B$*b,Z>AY`ZA[=W<V 2O_NH@8 `W184#HbO^ 8'<HO @Q5aVEaAU <IAE 4Y?.N>'<(U=a-+Ob-1 FGB3]N1-b4PB%Z( JG-NS4c3% M!!A5+;?5VGT@2V*!!%c">^[2?@ ;U7bVb-U8O!b)0>B0!_/D.ZGEZ ;_JRI4F nJ6Qni6,l;]4AZojQ #_& t8fT\z KSu'B6,8D D|~J: r Xb 3x{Nv.R7pZ^}*KHE_1W~HQNG7 % .O[j 8)F "S SqaA(,t}Z[FQI<Ceax zT6%ZO^sei'6%mceX[i{_pf.;8B  )~mw1%F.?X,1="s2T@ ,\LH[z)`CONZp.abWs\iHhu{J1TWkVJ7{AaZB!)K0 l7*S%Qsms2>i?BI.avP=}#z8"z!4R:g#.y; n-Pgi&*Mhk}QVFq&ej=_tzY]RF6I9(2:%"+.:!A 8'2%03< ,         x'           5) /K "8 :  <        B#.0+.&#535,A1-4J/9!)/N #%-_C(#N3*H*+'-3-37&1*$7*Y#Q<J,(557C39B):$-'PN.39)MGJJ28B#33+J=>>E,7 %7111W5555xp)|f > "p`:Kpi4,rIcWpXj0$DpEgZ+$0!w}IU ?z@I1;G)kLd5  R}!N"K 8;_2o|gdL K:o_l 6|d_?vhi8 %}4e'8TW8ex~(g? "NVYv{Fd/sdi]X>"dn~~5`p"L),X.pU?:$bJwpepCl%j38MX2!ppp~sE XJ  VL{|) gk/|X1)RWs:[ 6y5s!u4 <m+gu3%le6{Wt]rRr%H4 0dUJ{u'"TfP8KKINm?~@ aXZcTu"i> r,b).ZM GmU)rvN:<UG=,"]G0V**$ X%E(& #S6t{w"!d!>BYf8PdWc[P T._K<~WDVQT mRe%NfR<y!'oY?7R}AxcrWTe.a=o7lf~a_EbwK]EY+G]4y=w|"5X_>ZQZmNWY&qUCd-x?\s^nY| K-!K Z96KM8FO>M)fV7_Kn*waPJ;#oK\,zo|AAAAAAAAAAAGTTTCTACGCAGAGGAAATAACATATCCTTACTAAATTTGCATCCTCACTCCGAACCCGAAATCATGATCCCTGGTGAGCCACCCCGAGAACTCCTTCACCCCGGAGTCCACCCGAAGAACCGAAGGCCGGGGGTGCTTGCCAATTCATCTCCTGGCAATGAGACACCTTATGCGCCCAAGGAATTTATCCGCCTTGACTGCTACGAAAGCCGTTGTTACCACACCGAGCCGGTCCTCGGCGGGGAAGACCGGTTTATTTTCTATGTTATTTATCCCGTTGACCTCATCGACAAGGGGTCTGTTGCTAACATATTAACCTTCACCGTGGCTAATGTGTCTAGGTTCAACGTTCCGAGCGGCCCACGTTCGAGCAACTAGCGGATTATCATTTGCTAATTTAAAATCTTCCAAAGGCCACCTCAATTTATACCGATCCAAGAAAAATTATTGAAACGATATGGGCCGAGCCCAACGGGCAGGAACTATAATACCATGAGAGGGGGTATAGGCAATCACTTCCGGAAGGGCATGTTCTGAATGCCTTCGCGGTGGATGTTAAATATAACACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:37:37 -04:00AEPt?RAEPt?RAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt/ASPt/B1Pt.B1Pt.BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?n~TDATA ?n~ӠDATA ?n~R|DATA ?n~XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP<P1AMEg!P1RLEbP1WDEkP2AMEtP2BAEEyIP2RLEp5PBASEERPBASEEPoPCONEE`RPCONEE^ PDMFTPDMFPSPLOCEYPLOCETPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%P4SCAN@SMED SMLtSMPL!!TSPACAcSPACPLSPACAcSVER4.0SVER } SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHPjphDYbigphQLcphTRphTR?This is the confidence range *ABI_Limits..FVocAEPt?RAEPt?RAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt/ASPt/B1Pt.B1Pt.BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?n~TDATA ?n~ӠDATA ?n~R|DATA ?n~XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabrFVocaFWO_GATCFeat//2GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP<P1AMEg!P1RLEbP1WDEkP2AMEtP2BAEEyIP2RLEp5PBASEERPBASEEPoPCONEE`RPCONEE^ PDMFTPDMFPSPLOCEYPLOCETPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%P4SCAN@SMED SMLtSMPL!!TSPACAcSPACPLSPACAcSVER4.0SVER } SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHPjphDYbigphQLcphTRphTR4{,|Qv%_F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBI0E#9LyH\R(qTk#vml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MrrG]wQX,B]J}gskOitiSFoFWT#"u677^w@+>2o~*,p""#]eq }X'KD7I 9_[*?bCDkSOj!Q4M"}JMzR9JK[Yl)d762H#A#Q9U,Z:X=<;q4n%DSi^tpuze2#Xxiy?,1OPh~wA[h@tC]"#]}l~e,SXtmI^#lW4?/D:Q64Pot pN9IlVOY(xs/b@j/.=y`6@{u4f\TP/h;ZQkwqzFGP7T]V%R5;MmN2CgGz^nLoT.iPtj glX ,cW`e;E;qK|-Vz^b;0|>kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?W~jH((nN#qvGq312rYja)kWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8*$Ig{sBb0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme