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(/B-2',#-EOTcP!910HDG3,-$3$ 2-'  !+/- ! %'+,%!         ! ` *%" % !6)*%1$  %#)>(D(  0=4   +     !%/+&8/   d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}|zxwtspomligecb`]OOOGGGddd{{{   $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4cad0bb1ec10478abd708129ea736c4dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-Well#K1317_E10_Crusea_calocephala_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z|qe[\s-]tq[B6A_K .(JB2x~=K`i=$$;\x}g=G_S${yjxq[Xf}b E, 6P]T6 "lefo~W2 &>[qyshbi{zhdqdH;E]wx_F>Pw_5$4]_oH,"% rZLD<1$4Uxa9 /@HD6" +8DKMIA4&(>NRI9*#+Ad8`vHi3 !N}bLWYN_S2>vhS8 '>Wo|dF*$>S^[J2  *36/"  (*% (9@>2!      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" ) G+C#'  + *.!  'kI+HDB+#HW5D(d':,IO.-C0"139 ,),,!,, /,; 2 7-H,,r,--,--&=2'&P-,,-, Hd) ! E1C-o2dN222,J,,,,,l*s"'o8{dTvK 'pVvP&yc+Bkp|UG<,$}.s>GY!8*[<so}JtdKn#8g9'Bqbs,< vt%58@ K   o` P-pS UL`ne)k+,?~i\7 ]$BC-r_.@&,pyJ9dog8#p<Fgp%yE__G/~DZ*B[-W|H Q91J(ExkdyMWwkl(Dapp01EW2KFs,DYdpbEp+,ppa(3$,tq@Xo,/|*t!]~^CXi4"B %>-|qh }tq&+AkzUIgPgh/mn5#o\`\VxA,H.5dYQweyPIk/ tejO>XO;X p[f}&vO9o_tJ7}Eo9jW:rNT^kI~}%JZ:_Pxp@lP~`Y{gv~dJ>Z~ZY>sY}As|{Rel> tBT3Dk\coUac_r%.BU}sVR`1GorXmtNo!\N^TkG||=~Kl,_,lQ\":;r$3-ce3htrZt+>[9}BN;*3{8C*8lF%3;X)k-Gvq-;uce%z6ZIL]^klky= &F2-CGAAAAAAAAAAATTGCCCTCTAACTATATGAGGCAGAGGAGTTTCCATCCCACATTTGACCTATTGCACAAAACCACTGATCGCGAACCTTCGTGGTCCAAATAACTATCACCAACTGCATTGCATTCCCCCGGAAAAAGGAAGGAATTTTCTTAACCGCCTGGAATCGCTTAACCTTCTGAAATAAATAACGCAACGCACTCCATTCCCATACAACAACCTCCAACGGAATTTACCTCCCACTCACACCTACATCCTTCGGGATAAACTTAACATCAGCTACCGTCACCATCCAATGAACCTCCTTTTCAAGCGCCTGCTACAAAAACGAACATTCTTCCATCGCAACAACCAGAGAAGGGAGTTCATGCCTTCCCTCCCCTATTTATGGATCAATTTGATAATCCACAGATTATGAAGTTGCTGGCTAAGGAAGAACCTATTTTTGGTTAAGAAGTAGTCAGTAAAGTTGGCAGGGAATCGGCGCCCTTGGATTGGGATTTCGAAGACTCCTAAATTAAGTAGAAGCGCTTACAACGAGGGTTGAAACCGGAACAAGAGTTAACGCCGGGAGAAGAGATAATCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:38:05 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtAASPtAB1Pt@B1Pt@BCTSbBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <x&TDATA <x&DATA <x&EDATA <x&9DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~a>LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS5gP1AMLLP1RLLGP1WDLQ*P2AMLZZP2BALL^P2RLLUPBASLL7PBASLL5PCONLLEPCONLLCbPDMFTPDMF5~PLOCL>PLOCL:2PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%5_SCAN@SMED SMLtSMPL$$TSPACAgG#SPAC5wSPACAgG#SVER4.0SVER bSVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCH5phDYbigphQLcphTRphTRA聅This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtAASPtAB1Pt@B1Pt@BCTSbBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <x&TDATA <x&DATA <x&EDATA <x&9DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabq"FVocqFWO_GATCFeat//pGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~a>LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS5gP1AMLLP1RLLGP1WDLQ*P2AMLZZP2BALL^P2RLLUPBASLL7PBASLL5PCONLLEPCONLLCbPDMFTPDMF5~PLOCL>PLOCL:2PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%5_SCAN@SMED SMLtSMPL$$TSPACAgG#SPAC5wSPACAgG#SVER4.0SVER bSVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCH5phDYbigphQLcphTRphTRa,pEnO"#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? VCCHY<6(mu y-WLa)&:\.~x|MI9?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3WAd@EaZL)E^rkrOW9;(dC18{ZWFrbT;fQGKekx&#v3"qo,D`&Tj1t9z"DEoh90S*o_cM&+#u?GG5)ljD_P?Yf]4'Uw\Pcc{\A'ZvXTP>I_n@(Zf.kRr+4#3y4E^h9r4uoak"T'o7LA;mq;;9VgO(_y"`k:!.tkSY4E2 (# +6hjH(pT:yfMGk)__(uKr)YW [xinJil]ZCd]'DZNu@v0kc "V&S$~=}F)h>;-T)RJTZmU)P? sIe]XkF8+W>7.c$<\}:miPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NH!I$jUK+nGF![y]p9Jz*g#(Rgm&&d]wvPBv~,N;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS5gP1AMLLP1RLLGP1WDLQ*P2AMLZZP2BALL^P2RLLUPBASLL7PBASLL5PCONLLEPCONLLCbPDMFTPDMF5~PLOCL>PLOCL:2PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%5_SCAN@SMED SMLtSMPL$$TSPACAgG#SPAC5wSPACAgG#SVER4.0SVER bSVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCH5phDYbigphQLcphTRphTR