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(2,HL92! %+ )B97  $)" 2+52   3*$ 155,A   '     #$*# /0+%' !  $*66! &',.(  :9G(%2"* 0&F=X[AMB//.618INTdCMA8   (.=3 !:6& !,  @,.!$ '     ' &   # %   "$ # $ %   !  *)                   $     (I785 y6<&d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}|zxwtspomligecb`]OOOGGGddd{{{   $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8ed7a82d24fc466daa655b751e487b12Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-WellK1317_D11_Vittaria_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z  %$#7FJA/ /BLI8"        +MfkZ<   0M^Y?MLf'?IG3 24iEruG|;j6 /883+$ .FTUG.:[rvgL0(6@BJkJ@x]>Tnw(  "%5IYnj 0y{>e@i8#4@@3  4FLC0 *9FKF7 ;ig; %;KL?)  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"                  "&*           .2 $  2+'++1+".(.8")"3(22"1+,! &$2.22.#++;'+""   $$.312( 08888-6118881+2 %""  2;;1>3";+(#+"...+"1+.8+12$$$+"..$.2.)++'2""'81.,%61, 3&2.1+;8";+2338$."&+.6.0"&#&+#,+1#&+8;6;.&+'  0++$"2-$(-"  .$.0"" $$,1+"-"+3+""'$!  .("" ,")1$"';11'+$++."."1(" '$'"$"  !      "                  "&*  `"'VD ^:;CI-"?3:1X=7?75'7?J[RSAS@0S]Z)V/ /YYY'.QF=-2,+6P'BE?7\ b2+Q$ ! M%.:&H7 ./T2-"c='2Y`P(J#5\ @R)7;O5:N9(W9DZ-&c0*4^7 40^(dXO!]P! W@Z ^_N<>:_V [7NJ3-M;;# ZJ-4`"-_F$W6M&"Y`)0>I$3V\ ["KD![#9F 6N9. P8;68A,<%M`YZG!U 1DPR9-;[,2GML ^U8$AEMHA1#(0:K 8TTX*DAcX)-;"R;;CRD7:!,/G7Pc/Y=)1H#`B8%&MF+;$X2G@`*$VV5$W@_C+_2*c J"OKTM-<B9)8^&##9!;4CE@::U2: ] X'b'*3%aI 7,M\5O=)I4N6'S%,=&A& ],59"GMV;716LC$NB'49/UV=F`2BF@TF 92=2.=!++<F 'ujtGu4GQT5 ^[2^"NZO~ g`TT\\1?T)2,(Hk147p{ 0= _A'1&I|}n' nQv2( 8(I_}DdwWf.< cWJ v*,k6y4j#gtH*u&&f-F; )9QcG.mWh]ZWi> UwIO|"-^ShzXlNeH,o]LNacp%CXbBRs9U)zs 6O@5*CscJbcK.U+^Ay _K I[?}m<i^ytKG[h`9e?b(cO>-KDc}S :YNvp_S6;Ac& 2 <@Z7 d ( `R(/B`etIAR$9?>,+c69+9\*H! R5_)>) ^V4& =$2"([Z1&*OT(= [(/3!&1/%%-$T!.+_ 0  D>/;( Y} 0&<<WN5Gn,%;3Y$%D o8$=J ":*Dk D C!0'^uU',a_703H600000pjO+pJpyrg=tmgL*Y$f9,pg 5~Jjn]'O+GMV*-p? 0'?6:yd,z81Ptv.\8bxE  P>* _y'iQQFzBJ^%p.8TE!7ed L|D1 =l6zgG t3A//}VO8Z:M'#~[NK?Riu ripRue.tb;:Pgd>n1Ep!]aLCq41pP9qZ6GR /SHOp3EJ&uPH_ F*%~dd)*|@@-#PaS|~<51Sl;3>V^ul=$%:,FKm,W{sas{w Rc3=rMM`e%R)G8_Pkob~R\~voEN~Mt|S" G+[tsoaeke\lt j6H|]2d;do`fXREMBymk~K<tn<krl?2eGTb.,}N`6ZBz[suP:HUD}FJ[;tnY3\Q1M~mAX$fI E3"o@[t_D_0W*7<ZYsw>8[np[wCf/G07p0+h(c VtB|P# /VXphFjPKm.27C MCACGGCAAACCAAATAGCGTCGTTGTAATTGCATCAACGGTACGGAAAGGCCGTCCTAAGTTGAACCGAAACCAGTCAAATGAAAATTATGGCGAGCCCCTGGAAAACCCTCCCAATATTATACACAAGAATCAAAAACCAGTCAAACAGAAGGATATTTCGGAGAGTGATCCTTTCCGCGACAAATTCTATGAAAGCCGTTGTCGTCCTTATGGCTCCGTCATTCGCGCACGTGACCAGCCGATGAACTACCCAAGCTAGATACAGAACATGATTTAGAAACGAAAGCGGGAGATCAGTACCTACGACAATGGAAAGAAGGAGACCAGGGACTACCGCCCTACCGGCCCGAATACAACCAAAAAAACAACAGAGCACTGATAATGGCAATTCAAAAAAATGAAAACGTTTTTCTTGGCACTCAGGGAAATAGGGAAGCAGGCGCAGATCTCCGGGCCTTGGGCCGTGATCCCGAAACTGACGAAGGCGAATAGAAGGCATAGCATAAATTTGGTCAAGACCACCCAGTTAATCGCCTGTTAATGAGTTAATAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:38:19 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS?@BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 7oTDATA 7oĞDATA 7o4|DATA 7oZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~=LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AM,X*+P1RL,X%P1WD,X.P2AM,X73P2BA,,;P2RL,X2PBAS,,PBAS,,sPCON,,#PCON,,!{PDMFTPDMFWPLOC,X#PLOC,XPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%8SCAN@SMED SMLtSMPLTSPACAlSPACPSPACAlSVER4.0SVER ?5SVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCHnphDYbigphQLcphTRphTR8This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS?@BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 7oTDATA 7oĞDATA 7o4|DATA 7oZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabMFVocMFWO_GATCFeat//M[GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~=LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AM,X*+P1RL,X%P1WD,X.P2AM,X73P2BA,,;P2RL,X2PBAS,,PBAS,,sPCON,,#PCON,,!{PDMFTPDMFWPLOC,X#PLOC,XPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%8SCAN@SMED SMLtSMPLTSPACAlSPACPSPACAlSVER4.0SVER ?5SVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCHnphDYbigphQLcphTRphTR&T`XDG7(IG!Hgn+1Ab;*55p]?'KzK:*]Q{Y^@>=,8,XNKioh|m C]Z&|k1jK4|<0S1pPRuKojmqy=Y`gP#0beaA({]/wQ2a52rW.X}=1m)h>VO@F$^8c[_RNgJ&jVUFM2hiO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFGTF4kb0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme`T95P3&5L(-3]OUTt5a<9af.EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMKLbsMvF(wD1RuM]Ux+ +i,S"l@9+:GSJ<6DR+M%LQ/fa:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;N