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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}|zxwtspomligecb`]OOOGGGddd{{{   $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: aadcbd681dc24bc4908208992e6d1ad4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-Well)K1317_D10_Eleanthus_cynarolephalus_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z/ES[dt!01,(-?^bE0&$&% }wusphZI8(   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(3# & bL /3   #21 6z!/"z(H,!)   = 4 Y B%( -4  6  H/ %@2-0;?N"< A?3?'"'5&*%NKR&&.U= #'.. )'* $43NCS0244/(X!/SVD4UnHO/}@/(50PLd79b9&E*);`t@dIIII)O7II,F$5& z;,,IIj#JJI8x,,,b~N#JJ<J88?& 86,,,;"l+r&<@ 9999ICIJ^uIIIIKbl7Ippq5y+G3,I,p@Mh~7>LV<iO,[ .,v,X"fJsu"8 \wMCt B;a=[dMO,Es<W VAojTahb8@?Ks4;xic]#;1~~(Yx8d\n4H 8`"x Z1T+8+p>*x_:NdKrp[ICfZP#_E &pa>^{bBr8u}<-V,.p4%S +8 Z!*~~/N|e?+@^Mjl|*N_,.Kznon h?3@Qs :0C_HXuz&~qKm EZ tyxZ*B_qPsdrn"so<xL,1f3&-|t _C\\<`3'hmzhGfmmk8]Ju&LtE ^Qa,UvScrZim'6k-a$+m;iJ1Hwg{9O>km{Qvr]MpH>vp~l}SmVlkuh=-f~g\#^k+,q B3_;I}Gr]b<'atsXAAGGGAATTATTACCTCTACATGGAGAAAAGCGAATATTATGACCAAAGATACACCCTAACACCGAACCCGATAACATGATTATTGGTCAACTAACGGAGGAACTCCTCAAACGAGGGGTTCCACTGAAGAACCGTAGGCCTCGGGCCCCGCGAAATCCATCTCTGGGACATGATAAACTGTGAGAGCCTAAGGGATTTATCGGACTTGATGGTTACGATAGCCGGTGTTACCACAATGAGCTCGTCGTCGGGGGTGAAAAACGGTATATTTGATTTAGTCGCTATACCCTTACACTTGTTTGCCGGTGCTTGGGTTATAACATTGTATCCTTCCATGGTGCGAACTATACTTTGTTGAACATCCGTGCAGACTCCACTTCATGTTACAGTACCATAATCAACTTCTTTAGCCGATTTTGCCTAAGGTCGAAGTTATGTTTGCTGAAGAGACAAAAATTAAGCAAACGAGATGGGCGCGTCCCTTACGGTGTTGTCCTGAAGACCAGTAGTTGAAATGGTCACCATTACAACCGGCATTGAAGCCTCAAGAGGGCCTAGCGGCGGGAGTTTAGATATACCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:38:07 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt`ASPt`B1Pt_B1Pt_BCTS{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?B~TDATA ?B~QDATA ?B~QDATA ?B~YDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~zJLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AMFeP1RLFaHP1WDFj`P2AMFsxP2BAFFxP2RLFnPBASFFQ^PBASFFOPCONFF_PCONFF\PDMFTPDMFNPLOCFX0PLOCFSPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%NSCAN@SMED SMLtSMPL**TSPACAgSPACNSPACAgSVER4.0SVER {SVER,,/Scal@Scan@TUBED10Tmpr<UserphARphCHOphDYbigphQLcphTRphTR%-This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt`ASPt`B1Pt_B1Pt_BCTS{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?B~TDATA ?B~QDATA ?B~QDATA ?B~YDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab.FVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~zJLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AMFeP1RLFaHP1WDFj`P2AMFsxP2BAFFxP2RLFnPBASFFQ^PBASFFOPCONFF_PCONFF\PDMFTPDMFNPLOCFX0PLOCFSPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%NSCAN@SMED SMLtSMPL**TSPACAgSPACNSPACAgSVER4.0SVER {SVER,,/Scal@Scan@TUBED10Tmpr<UserphARphCHOphDYbigphQLcphTRphTR_R|!{VmgMm[*&`cz"B``,_bX@sWFa}TYKt9>,3HB! 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