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"4!(  !.'8C)%/+YNod,,,III^^^{{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}|zxwtspomligecb`]OOOGGGddd{{{   $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e18ced234eae4b658b3ad46df492691dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-Well#K1317_C7_Xiphidium_caeruleum_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z $+3<HYl{tk_SF9/'" yidfnw}~|vnf^WPKHGIMRWYYWTRTX]bdb]VQNPV^eknnljkox5Pjui`TB'qe[QD8-()/7?EGFC?<:888887631/../00.+)'(*.38=BHMPPKD<77<ENSQJ@7215:>?=<>FVmG_:G=D[#uW?0+-3662/1;Nh,APWR@!lVC4)$#'-12/)$""%&$ #H*v1m`4:}B)Rx)pl+hMlhA1{A     !#%$!     3{kP'*'$Wc:bu0k6,> 4faFQ$    #&$         -h"!0XeNhmfr2)~s   k  +0/' %l}.O^Hn!  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UYCRB@"N<!,>F X6Y 9Z 3bZ*WN]I-8 T[W] KF@$0%D]=/ZC`XG(FY TI)F-LH\T-9 b1a6<# c]3 N= XH^"MB 4%M:O/ !P_F=L3@MPX5S8'_UY_(C5 5(U];=_?Fa5 ?<+U" 37!VG ?E<9 *+QL]P26CG6 D<^]O@K#T(b%CJY[G! `N5L6(<XZ/#H]2da *:[2 MkBCV'&<Tt13]sAL^0S~wF~4+  pl \.F  #C l3m; R&@KPUK DGs mKtD=e(s{ N9+l>=O<H&|Z0X|pa?I  NF\"9H|yEb>.Hw)Fl' 7 m]+  B)p5`6][3q }76"%jz^0r6OS%#MX + *hcS4A>qvi{dva]RRK}#[aN,f`N s~Lz["npG7^ #Ix"V3z:>NuvC*!H> ,{ZJz"v>G H=0su Ii"z,)gEe|@[r* 8d0.0 H QiH;}Nn # sfc\kG=F7DC>49YR$?F)6(8*+/            &      )  ! $9!'  1%"-!3"!"$(-?  #6#0' (8 3$0.!4G75#)$#(@0V+`0B"'.=)._6A(J*2#"N%%%$%  *t ###{A`jLppx1Z[p>ItKHF`y ||gzgI 3Ls#pBrpm^l*d M{02%z`<9LZ"]gU&hpsOATO rpg,,~:gaDDIEdex;Eg8VD8NC8S#oE^aqpo-Z^'i8bnK<[&+}&p^"GPSp!C^,dUz9AZ{/@x=rp&EU4o,Ojk+g0\,7<R=k6ptf@ 3v^k-ty'IV2[,98g'k5)WE?K!* i$  .\  % *<)0&$ !'%*!&A#!(9#0%(! #"#( # ' # IaC37+ '!&  /2 !$)-? -!&!%*!1# )50 ?>.#!&$) '" "+&($B;"#!!#8"#(&!$-,&7&!&-&'&"(7,!!%" +! #%\4'$#/;#+#(B5-- -.0,7s* '(*6!": /#"!</0+:1 /J.)'3^-DbE40%QnD (;)3%2+/% &-+CB &1{:7%8+7=#$(K::,L[3"s8(8$:r9&7@E%)<7.jt+oeu>[WGAAAGAAAATCAACTCCTCTAGCAGAGCTCAGCGCATATTATTACCAAAGTTGAATTATAACACCTAACCAGAAAACACGTGCACTCAAGGACCACACCGCGGAAACCACACATGCGGCATGCCTCTGGTCAAAGGGAAAAAGCGGGGGAGGTGGCAGGACTCGCCGATTCTTCGAGAAGAGAGCTTGGAAAGCAAAGTTTTCCGATACAATTTGCTCGAAAGGAGGGTGCGAGAGAGTTGGAGTTTCCTCCTCGGGGAAATCTGTAAAGGGATCCTCATGAGCTATAGAGTTGAACTTCTTTTCGAGCTTCATCTCGAAGAAACATCATCCTTTCCAAGGTAGAACGAGACAAGGGAATTCAACAGTTGACAGAAATATTTAAGGGAGAAGAGAAGAATTAGGATATTTATGTTGTTTCTAATAATCAAGCAGTTATTTTTGGTTAAGAAATTATCAGGAATGTTTCGAGGGAATTAGGTTCCTTGGCTTACTCTTCAGAAGATCACGAATTCTCGTAGGATCCATTACAGCCAGCCTGGAGTCCTCGACCTGGATTATCTCCCGGTGTAAGATGGTATTCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:37:55 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt?ASPt?B1Pt>B1Pt>BCTS8BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?Z!DATA ?ZS{DATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR7P1AMGi0P1RLGdP1WDGmP2AMGvP2BAGG{hP2RLGrLPBASGGTPBASGGRjPCONGGb[PCONGG`PDMFTPDMFRNPLOCG[PLOCGVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R/SCAN@SMED SMLtSMPL$$TSPACAdŜSPACRGSPACAdŜSVER4.0SVER -SVER,,/Scal@Scan@TUBEC7Tmpr<UserphARphCHRephDYbigphQLcphTRphTR@This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt?ASPt?B1Pt>B1Pt>BCTS8BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?Z!DATA ?ZS{DATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//SGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR7P1AMGi0P1RLGdP1WDGmP2AMGvP2BAGG{hP2RLGrLPBASGGTPBASGGRjPCONGGb[PCONGG`PDMFTPDMFRNPLOCG[PLOCGVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R/SCAN@SMED SMLtSMPL$$TSPACAdŜSPACRGSPACAdŜSVER4.0SVER -SVER,,/Scal@Scan@TUBEC7Tmpr<UserphARphCHRephDYbigphQLcphTRphTRTrU]wfg6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! P8X-o_}4]'G' YHT+(aQQxnJilXSXW .C=vz^`%C];p3TVu>g\M9K^qyJsW~bH+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3udrVLgJRv)1mfc4hApvaUGxP=O_l;mqy=Y`ge#cme/^i1>QD/N:6M JBWLv'Pb:L)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.nzcZblujjmwnN?$OA2nrD5pYlGcRb[q5FaT9Vbr$rv/qNE^O{|cHT?Ou4|8e7spFp[O6h"4#O|!f|bEdG;_{8@hh/WZ#3|)l>,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*B1Pt>BCTS8BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?Z!DATA ?ZS{DATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//SGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR7P1AMGi0P1RLGdP1WDGmP2AMGvP2BAGG{hP2RLGrLPBASGGTPBASGGRjPCONGGb[PCONGG`PDMFTPDMFRNPLOCG[PLOCGVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%R/SCAN@SMED SMLtSMPL$$TSPACAdŜSPACRGSPACAdŜSVER4.0SVER -SVER,,/Scal@Scan@TUBEC7Tmpr<UserphARphCHRephDYbigphQLcphTRphTR