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$$$,,+333:::A@@HHGNNMTTTZZZa``ffilkqqqwvw|||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 286ca6969d2d434c8e4b948f922b56dbSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-01_20-17_07153130Seq16769134779_134779mfagan96-Well"""#"###""######"####"###########################"####################"#############################""##"###"############""##############"############"#########"######"#######################################################"##########################""#"###"########################"######"#############"################################################"######################"#######"####################"#######"####"#"########################################"#######"#########################"##########"#"###"""####"##############"####################"####"###############"##############################"##"#################"######"############"####################"############"#############################"####################################################"###########################################################"########"##"#################"#######""#####"################"#########################"##############"###################"###############""##"###"##################"########################""##"######"#############"###########"#"##########"#######""###########################"#############"##"######""###########"###########################################"##########"##############################""####"###########"############# 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"&&"     ,@eAA@E9AKB.bcpKB_3730_POP7_BDTv3.mobtermGGACCATGCACCTTGGCTCAAAAGGTAAATTCTTTGCGGATAATCCCAATTTTGGTTTAATAGTACATCCCAATAGAGGACGACCATACTTGTTCAACTTATCTCTTTCAGCTTGGATACCGTGAGGTGGACCTTGGAAAGTTTTTGCATAAGCAGGAGGAATTCGTAAGTCTTCCAAGCGTAGAGCTCGTAAGGCTTTGAAACCAAATACATTACCTACAATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCGAAAAGGTCTAAAGGATAAGCTATATAGGCAATATATTGATTTTCTTCTCCAGCAACAGGTTCGATATGATAGCATCGCCCTTTGTAACGATCAAGACTGGTAAGTCCATCAGTCCAAACAGTTGTCCATGTACCAGTAGAAGATTCCGCCGCTACTGCAGCTCCTGCTTCTTCAGGAGGGACTCCAGGTTGAGGAGTTACTCGGAACGCTGCCAAGATATCAGTATCTTTGGTTTCGTACTCAGGAGTATAATAAGTAAGTTTGTAATCTTTAACCCCTGCTTTAAACCCAACACTAGCTTTAGTCTCTGTTTGTGGTGACATCATTACAGGACCATGCACCTTGGCTCAAAAGGTAAATTCTTTGCGGATAATCCCAATTTTGGTTTAATAGTACATCCCAATAGAGGACGACCATACTTGTTCAACTTATCTCTTTCAGCTTGGATACCGTGAGGTGGACCTTGGAAAGTTTTTGCATAAGCAGGAGGAATTCGTAAGTCTTCCAAGCGTAGAGCTCGTAAGGCTTTGAAACCAAATACATTACCTACAATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCGAAAAGGTCTAAAGGATAAGCTATATAGGCAATATATTGATTTTCTTCTCCAGCAACAGGTTCGATATGATAGCATCGCCCTTTGTAACGATCAAGACTGGTAAGTCCATCAGTCCAAACAGTTGTCCATGTACCAGTAGAAGATTCCGCCGCTACTGCAGCTCCTGCTTCTTCAGGAGGGACTCCAGGTTGAGGAGTTACTCGGAACGCTGCCAAGATATCAGTATCTTTGGTTTCGTACTCAGGAGTATAATAAGTAAGTTTGTAATCTTTAACCCCTGCTTTAAACCCAACACTAGCTTTAGTCTCTGTTTGTGGTGACATCATTACA)3:FOXait(3ALXhu!/;JYcp|&3?LYes &2:EP]jt)6@JUan{ &3>HT_kv(5BMWco|!.:EQ^iu    ) 4 ? K V a n y    ) 5 B N Z e q |   , 9 E P \ i u   $ / ; G S ` j v    & 2 = I T a n { +6BLYfr~ $0=IUanz +7CP]ht'2>JWco{ !.:FR\iu)6BMZfr %1=IT`my "/<GR_kw*6BN[hs)3ALWbnz $0<HUbmy ".:FR^ju "-9FR]ft)3:FOXait(3ALXhu!/;JYcp|&3?LYes &2:EP]jt)6@JUan{ &3>HT_kv(5BMWco|!.:EQ^iu    ) 4 ? K V a n y    ) 5 B N Z e q |   , 9 E P \ i u   $ / ; G S ` j v    & 2 = I T a n { +6BLYfr~ $0=IUanz +7CP]ht'2>JWco{ !.:FR\iu)6BMZfr %1=IT`my "/<GR_kw*6BN[hs)3ALWbnz $0<HUbmy ".:FR^ju "-9FR]ft   1'2+1* !64::>/.'>/2/8//888*6>>')3>>3;;>;,;;3/.>>>>>>>>;;;>>>>>>>>>>;1>>>>>;;88388;;;6>;>>;;>>>*+6;>>>>>>>;>>>6;3;;;>;;>>818#$2;8808;>;;328;>>>3>>;>>>;>;;>;>>>;;;;;>3;;;>>;>>>>>>>>;>>>3>;28>>;>;>>>>>8>>>>68>;;>;>>8>>3>>2;8>3;>>>>>>;;>>;;>>>;>>;>;3>>>>>;8>;;>>>>>>>>>>;28.-0+.>;;;3;;;;>;;;883;;38>>>>>>;;;;;>;;;>;;;>>>2;;;>;;;;;;'8;;38>;;;;>>;>>>>>>>;;;+;;+;;+>;;>1;>;3;83;;>;83;>>>8>8>>>888'&8,,88/%32&">888>8>/3888/8>88>>888>>8/+>+16/688(*>>>4>>>644(0+/>6>>4/>1/::6144444+44.44/64::4644,4*(44416/44%*.22.! ('%'*2%(#! 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("   ]'`V!dL!M4$99DJ4 YL< DB_O%=H8(S"?IQ[?K,QB%\+A!MG\bDaSS_;C;a \?(O"L:07D '@#c> A\8,<.=FN8V0V  E7K3V `[J*RR[`[/T__BZA, 7AU5.3X1 27*bQ$=F>/OS&[0DY%MO1RXBZ Q $90:75O8P,I^(=_3 -%W+?EZ>bS4,0(I a;B&9L@(B8 'V$9`3&UF#B`8MMMPR ?:\1%I+.( &-.V0W6W6XZ@)<, +A1(I=Z;'W9F'$W!5V3.=MWH%U/4(4*;I.M .B%C 2#$([\>$8IY[0Q'L!0VVCH<EX([!`6!S$>EO)LD9 U[O!6 !O9XQSAO76.HR8#FEa+WQE[LT=1L74\3 =W9]Y@)Y/d0GEcC:H&>UGR;8$X&59,EHD(N,9E;Y3 I2: 9>2a72?V_2HBPb[/ =_\>j[t8;&BLfbSPMg~Kp :]#TtLbq?EA[82_+!$a#Zx${^iG6!MKj80G!qVM 5sCFKa /S 7VIWwl*XDk1J`jqp{2Qw3fKht N^0g[]7Uo,d)J"&~vou87x/G+|P3cNx `Tyv{;-FcVm1 \&Xv5 \|~dC/a .L&3kN*p>jfCRF]p s=5  k4$\{~5Ex?>a$RE43`-FT`,x$|6,H ~~9 q  $ yjI !-p1n9{2\-Y 3nm{_dYp|o^D\+3\7E$N:))3 %711$M *.     '   #  3 K  B             >   0 ', /#  "$(;  %6-' %r%,,07(+/"K!1D+565!<C(!++"L#=6...J)@..[t?'M*.-$(@&?W'@S<'''&'&&;.2.$2.''.L.cvR&9p8p~X>mUVL8p_ m| px3li qhYWG$$4E/A}s>'fD/S|k]1QW86 ,#,Hppv 3MTjs=ee, '"$,d>};S2xK6,!pfbZdh"z% <d+:t_>:=,8~|},)s9SLjs:A,0p@O?<Cp$$p lp@ ~C`'O&D- g5bKQpkCV y >[-L2  (ptLwLs]H}*lpRC',@'awGe[;etkW-WL[7EYSH{ L/*(M$Bi&;BFMS+CS:l@9=d*!<e3Q'UxRaH2Tf`BBgD14&6*(1)%"+:".Al^m =Rr.!;Cv'9I;eIIB0@&-](k#032c&HN`5Lv72;'*J`X5CJ=-U.85/!9y^(L*Fd<<#=]4=F"V8,>^kcA}bAte$9$"_>B.?>4=>BK=-Jeo+!+6<L:9h$,$DQ(]S-oV*-#Y%&$7f> .>5?3/l.p~:Aa'9Js;#>RgQ971|d*@AyVF!CBOV2QhzwZT5W+H:m'7g'4(Kr+8D9&K+-I8\D*7XfL"&e5N)F%3IcYAMuN%0QN/QFo&"=AB")C-IW0kp(>q)i03`a'o'fOc9\)h0)J``&d$T=1dwDVMdn9]ZHLr3@7bC&W38>KA@YtD3$>e/jj1<:H=)hj!5[[gs!-[]Apet_aroU,E"R^cD iQKAAGAATATTTAAGGCCTCTTCCCAAGTGTACTCAGCGAATATTATTACCAGAATTGCATCCTAGGACCGAACCCCAGAACACGGACATTCGCGCACCGGCCCGAGGAAACCGCCAATATGGAGTCCCCCCGGAAAAACCGAGACCGTACGTTAGGCCGAAGCCCTCCTCTGGAAAATGAACACCGAGGTCGTCTAAAGGACTTAACCGACTTGGTTGCTTCCATAGGCGGTGGCATCCTAACGGCACCGGCGGCGGAGGTGAAAAACCCAATATTTGCCCCATAAGCTATACCCTTAAACTCCTCTACAGGGGGATCGGTTACAACATTGTGTAGTTCAATGGTACGAACTAGAATTGGTTGTACAACCCTTCCGAGTCCACTATCTGGAAGAATACGAGAATTTTTCGCTTAAGGAAAAACTTGCTAAGGTCCACCTTATTTTAGCCCAAGTGATAAAAATTAATCTAACCAGAGAGGACGGTCCCTAAGGGTGTGGACTAGAGTACCCCCAAGGTGAGTATCCGCCCCGAAATACGGGCGGTAAAGGATGGTATGACTTGTGTCCGGGAGTAAAGTGAACAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 22:05:33 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt!ASPt!B1Pt B1Pt BCTSoBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?XTDATA ?XDATA ?XSjDATA ?XDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR"P1AMKiCP1RLKdP1WDKmP2AMKwP2BAKK{P2RLKroPBASKKTPBASKKRUPCONKKbbPCONKK`PDMFTPDMFR9PLOCK[PLOCKVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT /RUNT (RUNT )1RUNT  Rate 0?RunN""JZS/N%RSCAN@SMED SMLtSMPL!!TySPACAdڰSPACR2SPACAdڰSVER4.0SVER dSVER,,/Scal@Scan@TUBEH7Tmpr<UserphARphCHRPphDYbigphQLcphTRphTR$9This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt!ASPt!B1Pt B1Pt BCTSoBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?XTDATA ?XDATA ?XSjDATA ?XDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR"P1AMKiCP1RLKdP1WDKmP2AMKwP2BAKK{P2RLKroPBASKKTPBASKKRUPCONKKbbPCONKK`PDMFTPDMFR9PLOCK[PLOCKVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT /RUNT (RUNT )1RUNT  Rate 0?RunN""JZS/N%RSCAN@SMED SMLtSMPL!!TySPACAdڰSPACR2SPACAdڰSVER4.0SVER dSVER,,/Scal@Scan@TUBEH7Tmpr<UserphARphCHRPphDYbigphQLcphTRphTRV"O`x83E> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49WW!'YA ruO2H\,$z$%r%\\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUOW'cx+ne(AGj3B$&z2yEn~nl(}wp7Q9u>9"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\f.\uXs0NkKq;{[c&hikgG_&QEhXBNe<}M8XDo#UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4