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  $$$,,+333:::A@@HHGNNMTTTZZZa``ffilkqqqwvw|||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 15e2157e3e9f4c638434b160580955cfSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-01_20-17_07153130Seq16769134779_134779mfagan96-Well"""#"###""######"####"###########################"####################"#############################""##"###"############""##############"############"#########"######"#######################################################"##########################""#"###"########################"######"#############"################################################"######################"#######"####################"#######"####"#"########################################"#######"#########################"##########"#"###"""####"##############"####################"####"###############"##############################"##"#################"######"############"####################"############"#############################"####################################################"###########################################################"########"##"#################"#######""#####"################"#########################"##############"###################"###############""##"###"##################"########################""##"######"#############"###########"#"##########"#######""###########################"#############"##"######""###########"###########################################"##########"##############################""####"###########"##############K1317_C7_Xiphidium_caeruleum_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z,?KWew!''&(0=LWZRC0~l[OKNV^b]Q?+ 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AsN' $K+|TGY(:-P%   Cz"4(X- 'Q4H>b2  5^tG%4\mL>Ijc9  >ewP/ 6ehG@W"1"S,         'P%e=    /WuI' 3^%0 Y2   4]^92T|_9   +T$70j;    %BfsM- 5XeQN^}|V5   !8WxmM1 /UzaYc}cB(   1TzlM5" ,Jkz\>%  5ZhF+    ?mzR3 $DkiE*      &Fo[>(   (0684,"    #*00+#                                                                                                                                                                                                                                                   @AAIAKB.bcpKB_3730_POP7_BDTv3.mobtermGGGGAATAAAAACGGCTCTACGTAGTTCTTTGCGGATAATCCCAATTTTGGTTTAATAGTACATCCCAATAGGGGACGCCCGTACTTGTTCAACTTATCTCTTTCAACTTGGATGCCGTGTGGCGGCCCTTGGAAAGTTTTAGAATAAGAAGTTGGAATTCGCAAATCCTCCAAGCGTAGAGCTCGCAGGGCTTTGAAACCAAATACATTACCCACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAAGGGTAAGCTACATAAGCAATATATTGGTTTTCCTCCCCAAGAACCGGCTCGATGTGGTAGCATCGACCCTTGTAACGATCAAGACTGGTAAGTCCATCAGTCCACACAGTTGTCCATGTACCAGTAGAGGATTCGGCAGCTACAGCTGCTCCCGCTTCTTCAGGCGGAACTCTTTTTTTGAGGAGTTACTCGGAATGCTGCCAAGATATCAGTATCCTTGGTTTCATACTCAGGAGTATAATAATTCAACTTGTAATCTTTAACACCAGCTTTAAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACAACAAACAGGGGAATAAAAACGGCTCTACGTAGTTCTTTGCGGATAATCCCAATTTTGGTTTAATAGTACATCCCAATAGGGGACGCCCGTACTTGTTCAACTTATCTCTTTCAACTTGGATGCCGTGTGGCGGCCCTTGGAAAGTTTTAGAATAAGAAGTTGGAATTCGCAAATCCTCCAAGCGTAGAGCTCGCAGGGCTTTGAAACCAAATACATTACCCACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAAGGGTAAGCTACATAAGCAATATATTGGTTTTCCTCCCCAAGAACCGGCTCGATGTGGTAGCATCGACCCTTGTAACGATCAAGACTGGTAAGTCCATCAGTCCACACAGTTGTCCATGTACCAGTAGAGGATTCGGCAGCTACAGCTGCTCCCGCTTCTTCAGGCGGAACTCTTTTTTTGAGGAGTTACTCGGAATGCTGCCAAGATATCAGTATCCTTGGTTTCATACTCAGGAGTATAATAATTCAACTTGTAATCTTTAACACCAGCTTTAAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACAACAAACA )4<CN[grz '4@KWer~+8GU`lx#/;IVdq~ %1:DP\it &3=JU`mz $2<GR^iu %2>IT`ly)6AMZeq~   & 1 < G R ^ j v   % 2 ? 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I VA5J79IQcMd:SZ V2   ](^.SYEMN9[3.M' A),4-? ]R b> ?TV _ O)1W,FGY49%HRT9A"(O51%;QSCG 5<<JNM[.Z(+[W3ZT.PTUa?NZ!>^#VFC;b<N&*j2nE&uNM9~xV (HOV\[Siw|!8,X<YM'?w`B0gfh%gntnKzKz|P[6B@TV]*\{W z (5-vQ7%Pyy=tD m:2n"6 8 FTO[PKN{3-|}kMZW LZN 7((4HQ(*\<iO;Dt'`M6 s *jy#aD41hQD7bL VA>*Gg=~WmI~h(?JB P . s+KPa`#>(N U:+!-':x. :X<]v Lm,CNs9H&*b-=FK(R!!dw/yXnncN9U_ H w][UhL>#yqflvlfq~D.:6T2,1(B)8E&  %, !      1*    (! #          '" '%0 !I '&*:?%  !+/" $I'  ;5%'E$%)& &0(3>"*L$+>*9L)*&20:7&'5(&94''@'DpxpVwKppk1CIUdgp;b?pp,oZ>=,a t/Qp8$Sx,8#_*-fz x sd#8C i( w.ei!Q#RQkL[k18d %e8FBQ O~8 >6N  QE,Hoc .)pppR=\dp%fmUd:\ X)[pZ_2hp{tzwpDDeC_dm;Wp6;X&v7l@MYI3?j;.~Ud[$%+08(450Lyh.$o3%-Nj *%O0  - %!',"!#*%' !!!(% /%3$) ! & &1  %"X( *$3' $* "f '&*"(#%#$&&-$!$"''&#7$ 97,#!0&&%%-"$(")<*&9&$1*&B!' -!Cc+0!-&#0!4,("!J-'()(<,)&-&#$'8*@2''+<&<g) *(!-%2*C&A 25#/6A%#*AJ%=Oy"J~ =94>9!?C-EY-7(<F&2<+#;<CF?&>GS%7?=bT*6V3n'4#13#6032^/) "*21"GJ&828%;4';I ',8AEH1C.J7_KQ,)A)O02A2O[6nkQMGeAAAAGTATTTTCAAATCTACACATAGCTCAGCGAATATTAGTACTAAAATTGAATCATTAGACAGAACCATAAAACAAGGGAACAAGTGAACCAAAGCGAGGAAACCAAAAAGACGGAGAACCGCCGGTAAAAGGGAAAAAGAGTGTGAGGTGCAAGTACTCGCGTGGTCTAGGAGCAGAGAGGTTGGAAAGAAGACTTTACCGACACAATTTGTTCTCCAGGCGGGTTCGAGCGTGTTGGAGGGGACTCATGTGGGAAAAATTGAAAGGGAGGCTCAGGAGCTGTATAGTTGAACTTCTTTACGAGGAGCTGCGCGAAGAAAGAGCATCTATTCCAATGAAGAACGAGACAAGGGTGTACAAGACTACACAGAACCATTCATGGAAGAAGAGAATAATCCAGAGATTTATGTTAATTATAATAAAGAAGCAGTTATCCAGGAATGATGAAATTCTCCGTAATGTGTCGAGGGATGAGGGGTCCCCGGCTTCCGCTCCCGAGTATTACGAATCTGGGCGGGAGCCCTTACAACCACCCTTTATTCCTCAACCAGGATTAGGTCCAGGTGTAAAGAGTATCCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 22:05:43 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ? TDATA ? DATA ? RDATA ? DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~|eLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMGgP1RLGcXP1WDGltP2AMGuP2BAGGzP2RLGqPBASGGSgPBASGGQ PCONGGaPCONGG^PDMFTPDMFQPLOCGZ<PLOCGUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT /RUNT (RUNT )1RUNT  Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL$$TySPACAaSPACPSPACAaSVER4.0SVER }SVER,,/Scal@Scan@TUBEC7Tmpr<UserphARphCHQphDYbigphQLcphTRphTR?This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ? TDATA ? DATA ? RDATA ? DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabIFVoc8FWO_GATCFeat// GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~|eLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMGgP1RLGcXP1WDGltP2AMGuP2BAGGzP2RLGqPBASGGSgPBASGGQ PCONGGaPCONGG^PDMFTPDMFQPLOCGZ<PLOCGUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT /RUNT (RUNT )1RUNT  Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL$$TySPACAaSPACPSPACAaSVER4.0SVER }SVER,,/Scal@Scan@TUBEC7Tmpr<UserphARphCHQphDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?z#`D5^ivf0.l{W;GPTf]+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f.\uDs0iLK_6j`;kO/5Bj_]jPXRLUH}M_\BM?D$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9vAMV^@5oOC&qN1;g8kY+-^m]ZOQ>\G`V7%jxAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDT\B1PtB1PtBCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ? TDATA ? DATA ? RDATA ? DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabIFVoc8FWO_GATCFeat// GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~|eLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMGgP1RLGcXP1WDGltP2AMGuP2BAGGzP2RLGqPBASGGSgPBASGGQ PCONGGaPCONGG^PDMFTPDMFQPLOCGZ<PLOCGUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT /RUNT (RUNT )1RUNT  Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL$$TySPACAaSPACPSPACAaSVER4.0SVER }SVER,,/Scal@Scan@TUBEC7Tmpr<UserphARphCHQphDYbigphQLcphTRphTR