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  %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 127ab05ee8484e63bb28a551418138a2Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-WellK1317_G1_Begonia_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zfip} .Rt=l3w #HrxQ?GiLeke\Y`mwweA 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[-Z07>4Q9][E<6$M-;] E5\_NH^;.IV10@CY .H/Y?$R!2KE E<6> L+& 9 R<:< 9U+=% 8B":5AWV <K<<TK9'WX []H[G2K =*b`\SRKX N&%I2V R@Q QaL% IA^2UF6 ]`BB1)/7O2 205]h-7yem 2{B)O$GgkY>$}JZ5be<WI@g7F3HLPG!!luZQ,3;`OqcY;)5"7H43\6@+sKs^ygD}Qm9cf&f3|]zMl`ag@Be ;Ayt q6}tvS^6Mng|EEYLV e _52u'_{0RSM uh9H]QW18FZ;u=M% =P x_N*[/6Ypr'iB7l3E[KN0JE:m*Y~Rin(rR]A*Ews\-1*&3$myU*,QRB9Ydq)Lw<G,_I0#I4' 2409#VI36f\FOkkvxr;\_Jwc>ThbhJc(D"*&!90#!# . ) #    >x<7$ ,"" "$: "'*: 0*2 ".   +M! ?%(2F 4!  9 1$F02]<&g r Q&/  k"  R7)0<-;- 0*`, 6q& [3,-(## #OSF#(6j,*##7(12***R <;#)((####I##*# MpjpCp.9_@pdpu2i'"pOdz #W(qK CeKp4Q/}ZP9cB8l@W>D(>j?x]'j4g$(_CcIJHC6>p"o,pGB*+y"d}5EV, /:w?I#qXL !Ri@Xrw4,O\v/')>Hu4%1m:DxF}8Ku|k , vzEe?k5^/ @>WRa ~`6m-|>]G`nD65dtaSZ~%4^/[(cUl' 5LW{C o  YLK~"}O1|!V9XP3/`C+0$*.R?Y.D&7u,RM)6 A$99")(40##"Q@5-42(0*'21m9%=KB*':-*7,M-&l &%X-[A"}6/7cDC.UBF5*N$R81(Zf/,40|U>'4W6TdXXd=H8xj^D8jQLcLed17-GJ/JoLl?>JEcqk]Mhft;j^\wIpjRM)ug4bU@E_Tp?@sUfJQiMF<+o+\S~fM]MnT(wCEQJ:rL\\GHYk<m73w\BYYGqTa\ahQ7jNJyIIJ}Y%J}F[NWr[7~@eW4Ps5gTMH;UUd^kcr`[tDqSqlh }Y[N]monWhC&e+Hlu^0^]};LrzCPD gWP_hP[r8Y!"sCc"2`w8vLP:d+;MM0uHS746]]'l LF^tGGGAGATGGGGGTTTTCTCGAGGTTGTGATCATAATATTTACCCACATAATATACTGCCGAGTAACCCAACCGATATTCCGATATCAACGCTTCAATATGAGTGAATTCTCCGAAAAGACCTTTAGCAGAAGCAGTCATCGTCACTACATCCGCTTGAACAACCAAAACCACCTATACCTGAAAGACCAACGACTCGCACTACAATCCTTGACGACACTGACCGCCCTTCCCATTCATTGAGGCCGGAGGTTGATTATCTTATCCAGATACTGCCTCCGGCTCCTCTTTCCTTTAAACCCCCGGACGACGGGATAGTAAGTACACAAATACATATAATGACTGTTAAATCGATAAGGTCCACGGTCATCGAAGAATTCCACTTGTAGAAATACGGCTTACTAAAGCGGGAGAGCGCAGGAATCCAAGGGGTTCTTATCGGGGTGAAGGATTTCTTGAGTTAGTGGACCAATATCAGCATTTTCGGCTATTTCTGTTGGCTTTTAATCCGACATTGGGATTTTCCGCTCACCATCACTGGACACCAGATTTGCATAGGGTAGGCTGTTGAGAGTTTTTATCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:47:42 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtlASPtlB1PtkB1PtkBCTSQtBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9sTDATA 9sȮDATA 9s<DATA 9sDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~OLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS$P1AMF;uP1RLF6P1WDF@P2AMFIP2BAFFMP2RLFDPBASFF&PBASFF$PCONFF4PCONFF2]PDMFTPDMF$PLOCF-PLOCF)EPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%$~SCAN@SMED SMLtSMPLTSPACAm SPAC$SPACAm SVER4.0SVER QiSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH$phDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtlASPtlB1PtkB1PtkBCTSQtBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9sTDATA 9sȮDATA 9s<DATA 9sDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab_FVoc_FWO_GATCFeat//_GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~OLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS$P1AMF;uP1RLF6P1WDF@P2AMFIP2BAFFMP2RLFDPBASFF&PBASFF$PCONFF4PCONFF2]PDMFTPDMF$PLOCF-PLOCF)EPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%$~SCAN@SMED SMLtSMPLTSPACAm SPAC$SPACAm SVER4.0SVER QiSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH$phDYbigphQLcphTRphTRTrU]wfgM6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! 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