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   &+$ "  '      + &$     (  0    %   1        !# 3        %        "$",,III^^^{{{&&&999DDDXXaaavvv      **3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}~|{xvtromljgecaIOOOGHHdde{|   %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fb7e83555bab4c48a42bbfbeec18f5b3Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-WellK1317_F5_Iresine_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zW\fv.DJ=!LP92s  ,SV*Z !#n%*&()+;,;,-t-----g-,,S++W*)('&c%J$i##W""b! 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J)+1I9K!'> 40EE 8c  I/9!# 2.%'!9$,) & , +;NdV94  6)jN 2HLf& &K1 d410 /z<EU -/G]-D+cP=Z-'-, 04sO H(&RIOP!v3;(3*,,|a7.b4+,@,..hq< ppp!@n-HpV]ph%u#Tp8dTYP8Zy\n>8],u8 _pDyueQ';8RoUX}h"6LSvHCd`  0'2Guc<&DB(pz7[.8Kd6xVYtt:Dh x[]emp,`f t=6rvp3YEH;AXNNWS`g#R2P|>"_)~;Kj\GDlG(u/v<,&yEJ0ep*e*G+{,FpFpCRRp D Wr* H",P, :2' C o <]P] (J,p, P@#D2$B!f!1&[D ~KT^_4k<M~u_|(i(F~k)aM!fs8-:}4A(?oB\0e->=QofZKM;-B_GSP(6ATol]:5H\Qk7DEJ48p^.hzA)Xk0zQ5BaHVEo1]aVcM8{ui<e<A4PMvkzXg_x8]HAu6C,RO_?1KIa8\5;cpCR2FLc\azd;47biiQBf{,|3PjTm{W|&t_P\@^U<2FZN:N^kXTtz\plQbq=Vr__P@x}8kwJKDSbpNs*.u<x$oCZ_pX5r_f3#%>N\M?xLSw?Gy[?FKob~(\=*uN(NIF8uT6_:=qUaxE{|U5riU_\xt< v;wB;*d}7fGxFf~IB>F8yfOqb6 PHK$1cGGGGGGGGGCGTGTTTTAATAAGTGATCGATAATACTGCCGGGCTCATGCTCTTCCGAGTGTTGGAATCGATATTACGATATCATTACAATACATAGAGTCACATCACCATCAAGAACTACAATATTATCAACAACATCCTCAATATCCTACCCAACCTCTACAAGTTCCAAGGTCACCCGATAGAGCTAATAGCATTAGAAACCATAAAGAGTGTGAAGAATAAACCTACACATAGAAGTCCGTTGTAGATGACCCCAACTATATACACCGTACGTAAAGTATACCGCATATCCACCTGGAGGAAAATCCGATACTCTTAAGACTTCATTCAAAGTATGTGGAGTCAATATGGACCCAAATCCGCGAAGACCTCACATATAAGAACAGATCATAACCCCTTCACATATTCTCTAAATACTTAGGATTTCTTTTAACATTCGAGAATTTACACATCTAAACCACGAAGATTGGCAAATACCGGTTTTATACGCCCGGTTTACGCCACTCCCGGTGTTATCCTCTCAACACTTTTGAAGACCATGTCGACGTCGACCCCCTCGTGGAGGAGACAAATCACTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:48:12 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt+ASPt+B1Pt*B1Pt*BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >n|TDATA >n|њDATA >n|NvDATA >n|RDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~sLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISH6P1AME_P1RLEZP1WDEcP2AMElP2BAEEqCP2RLEh/PBASEEJPBASEEHiPCONEEXLPCONEEVPDMFTPDMFHMPLOCEQ}PLOCELPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%H.SCAN@SMED SMLtSMPLTSPACAgYgSPACHFSPACAgYgSVER4.0SVER uSVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHHdphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt+ASPt+B1Pt*B1Pt*BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >n|TDATA >n|њDATA >n|NvDATA >n|RDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTablFVoc[FWO_GATCFeat//,GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~sLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISH6P1AME_P1RLEZP1WDEcP2AMElP2BAEEqCP2RLEh/PBASEEJPBASEEHiPCONEEXLPCONEEVPDMFTPDMFHMPLOCEQ}PLOCELPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%H.SCAN@SMED SMLtSMPLTSPACAgYgSPACHFSPACAgYgSVER4.0SVER uSVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHHdphDYbigphQLcphTRphTRV"O`x83E> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49WW!'YA B<\}2b=aL?$}`Mc_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhlh9eD>0E#5cy3YRm T5E8ml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~fAX&4FtOFpti[$AP(m"`Tt]94|6@ Ki6t{_"#kt72We<>/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#FmFiAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt+ASPt+AUDTB1Pt*B1Pt*BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >n|TDATA >n|њDATA >n|NvDATA >n|RDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTablFVoc[FWO_GATCFeat//,GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~sLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISH6P1AME_P1RLEZP1WDEcP2AMElP2BAEEqCP2RLEh/PBASEEJPBASEEHiPCONEEXLPCONEEVPDMFTPDMFHMPLOCEQ}PLOCELPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%H.SCAN@SMED SMLtSMPLTSPACAgYgSPACHFSPACAgYgSVER4.0SVER uSVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHHdphDYbigphQLcphTRphTR