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",,III^^^{{{&&&999DDDXXaaavvv      **3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}~|{xvtromljgecaIOOOGHHdde{|   %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9fe91b1dd94b40b48a1975db2dad0018Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-WellK1317_F4_Miconia_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z =h )W.X G8\ Myr >6e!fqH* NW1FZmxZ5iF/->XlmRQlP9( |pe\Y^j{o^UU]goqpnopngXE5.5IdPQ1Rp[2{{8Fv0S;iIdcS(@qo =OGK[f]1hYSNE;218CLLB/ ')% 1Ig# <diT+bG^E/2Kanpi]QJJPX`aZJ5"#099.   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;2;)#8 3333388 *''&&82+   '6'$/ #*C]82SN%DM0 P*+F=8=;J7S$[ ^!G^&RD$9FQ]1[0:!,587?^&'*Y1+@"?.c'026cU SO`YCSSa6;?6%[,R1.8G3a^c W>\@IO*]S#-/Y[>=V9.)`C2(8=dc(46*B?P -<I&N 6 O@<TZM A6,L;P FW_6.CP<S ]](UK3/ &_(D M4QNa]A[ =U3=(J5%7S  VG#:&7\4:NWY]4SD 6"a+bQdK[&WFdO5T% a *:Q#%aTXVaBc:TaC"S?^8N#>":J/@`B/O(< "MYPc&T!0 .#P0 \>M, #C5[><ZVY7"!D'I0Z L^/6[3+$D=D''" (U9_6\N$R*1 E<FMFN*A3>$S?-$d= /3>, 6&=V+Q0PLaP[YK'>HP ^X$> 67C\6PX4>!YVFCH%B(EC*8+8#/5U+W?U8 @2^=2R UKWT?Z5&, b},r6L`Es5 {]Huu|}"F Z;M"SIp6vS \ w?:#B#xE5!0@{{aa3{mI(d<YWJK38I?j> i~/ i37(?1= jf]SU$i%Z s9`Fx?o`KrEpY,s\KFZ%9/IEAlX^>iOp00]&ct@f\tD*ZTff#=H F~uOj?_^ "ufbEkKMbHgJIn|8Oi;oY'WaY[,$ <u_ab)ME'ZO\Ee1[%=[zC0P/9o"j2JO u^oIJN0E<J8_~fRMx7 Ed RFPF`_mgfG!K,"fsz0]@*K+s4gr?`(Q2(A,3* 5 )A!,=,^[19 D%   $E <  2 %3 # $&" {  P( 5 *# $ "2'" !'  -   IEQ  O-( :,D ') ,m'F?, KD -%!z F -c +K/(If6,@zpvsupld8`88,7$G6pDw3zq3X9)br 3SD;6Ct8;} ,{X"-^%,-.+m+7J;K"|?a`BiW"sbj)LtcIH[bF74*/ OrZkQcDA!-M|?^I;F3K'm;=|`NO{+< nZUy,Y4E^ zQp 8RG<,+-+h1wSw*d)\B*W1 zSAmjny<}T0\zpRK((", Io_ }j`HUHS=gobdhjSN<m^/QkEydQw`+fU ~EbNH6:]]?p0w^J<H?;03o,,@5B,BP5G=0CV)7FhLK8#,:L|U-`NrL<1/+?G_CZ6sxB0V.I30MO.]<[::_J/q8^[>2,5=0&K5Z6lAG"iZWEPGaST< 1B7L48q0I7K,.pU6:UA[s_A[YgNgANZBCDEr_dZ^*G=1,qB~:Af.]_4wMpi?A5?FOgxHKtTbks;<n9+zE8*TKfvlVK?S2;3?GgYdJ.]SEc?<Q)jycE6uWm^[LOC'OuH0(]M_Z6oO^\Vz?KYKj]_MS\8N<DlP=T@FB.31=8G=nadhWV i_IjPi:`vEiYWGH_p;SSTk>esOYcdST:Vd `H%6@`dW4yMJOBw}hnV`j~ l`3Vni@QRN=\iC%gbAGGGGAGGGGTTTATACTAGACTGTTGTGATGGATAATATAGACTCACCTAATATCACTGCCGAATAGCCAAACTGGACCTCCCATTTCGTTACCACACTTACAATCACGACACGATAAAGACTTTCAAAACGATTAACACTCTCCACCATTTCCTCATTAACCTTCAATCGGACGAAGGCTAACGTGCAGAGATGTTTTCCCAACAATCCATAACAAGACCGATGGATAAGCTTTTACATCGCAAGCACTGCTCCAGTAGGCCAATCCCGGCTGCTGCAAGTCGAACATACCCCATTTCCAACATGAGGAAGAGCCCACACTCACATGACTAACTCCAGAGGAAAGCAAGGAACATTGGACCGAAGTCCTCGCTATATACCACATTAAGAACAACGGAATACCCCAGACTACAATCCTACACTACGAAGGTTTTCTTATTTGGTACGAAATGACATACATTCAACCGTCCAGGTCAGACAAGGTACAACTTGAAACACGAGCGCATAGTCAGTCAGCCGATATTCTTTCTGACACACTTTTACAGCATCTCTAAGTGGAACCTCTATAGGTATGCAACCATCCCCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:47:58 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt;ASPt;B1Pt:B1Pt:BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?VTDATA ?VDATA ?VSjDATA ?VDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMLiIP1RLLdP1WDLmP2AMLwP2BALL{P2RLLryPBASLLTPBASLLRQPCONLLbePCONLL`PDMFTPDMFR5PLOCL[PLOCLVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPLTSPACAfRSPACR.SPACAfRSVER4.0SVER sSVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHRLphDYbigphQLcphTRphTR<This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt;ASPt;B1Pt:B1Pt:BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?VTDATA ?VDATA ?VSjDATA ?VDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMLiIP1RLLdP1WDLmP2AMLwP2BALL{P2RLLryPBASLLTPBASLLRQPCONLLbePCONLL`PDMFTPDMFR5PLOCL[PLOCLVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPLTSPACAfRSPACR.SPACAfRSVER4.0SVER sSVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHRLphDYbigphQLcphTRphTR,9drWdG| nG\|8 f&hPVqzgN$6`a'K-n?5uHAId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?owO9KfPZ2V~oL`H9R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb?j !jCW8066X?J`GVR_6MW!w3[s2G[=ih2[-?Si#P6[s$$K%v'nE918sIRD8z{S>!lXS:Q&:C!g61CfFc':Z^mDt4)Mz9n#m+mb+peBRCE}dc-E2+5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,~sD[V$! 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