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",,III^^^{{{&&&999DDDXXaaavvv      **3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}~|{xvtromljgecaIOOOGHHdde{|   %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1230a902e0c14179be53c1af4784d458Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-WellK1317_E7_Asplenium_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zKVif Ox{mo\ n0v9 c D  1 M i  H v f l Z ro%%ns#kyA|2;]sZ-=GQa}S  /JmY$Qxa9)%!}wk\NHM^zjQCCKW`b]RF><DVr-^"CX[H#iBjPCCKXfr|~z~|vQ0u 94dsb; #N-{"GE{e]_ejkf[K8%&-/,&)6BHH@6.*-4>IXn<{zGdQGC?8,  !#    @{h ^,d?$ *00+#   WfPx\@+0(  5EI?,     '0.$     GxMV NiF88>BA:0'!   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3G (=E [:S<$\_(0# LK S@"9L/9_2D`+F@[ *)% +=QYS2'ULP+J/OHL>,R!W @CZ1\J>",;7HAPJ9A?L;?(FZ?\GT:H;L\) !KX.\SX"V7a.J]ZQDBA=?8a=2H\=-!* 6_c"7^+OUM 6Z#_Ua&W4*P*_O[]3](((%.,R.U1TO;)"+ `YRKCB+F]D@d/A %X#aKK#' 60E]6*=L1J?IYDIJE 1 'OJ&UD(<-L%8 ($$KSY H93QV*O [U%(]2OH2;! %].5`.L/0-O<: b M*D^.X(1cB>]I>:!6"$P K!"PMF 2JTV= <T/:, *$Q3SIX12EY!8,2` ]5\9D' @' IT=' B0/*$Q ^c.M1HO-U\Z!k"tV _ QMXAN7yhv>BY G=QxiW\=`Q;QQ N+mdLHCB=,/d ?*JrfEM aS>]JosRS7T'c~b bie$=UB=z<>)MY4- a""5#v<H-aD'CydaVWjA& .w\](~& {MP/9*MA MR.qf pg~9K\i3UW 1aC33e2,N n9vW iw:!>3!DD9[e'xjbYA ItOZ&.:gzYO] HkJqCn><9._d Ims}=4KKs0GJHfMPQ =%1j(e4E(1;!#%   ) E  '  < ;' B+ 2  "  <%  #.GA* )) %'/= 4 U>G . d*, D: >)5,K2/*13? 2--. +4 9&$(($+4M!!A =]    )Y(4!!4!!?!((H$~pmV+[+=Cip7DG@,pPl@?v,8/,/?Z,p+8{K~)3p\9Kdx ,\8 ;@p);pc;ppW, p8eCo@f2Pp*pG ~)<$1yUd"vxSxsE dUx[ju(88 .DpAdVS_RpJ3tEgl86lWT{c1*d6,g7y,D^d\7R 2m\'-TU3T,j7#->&t>>^'<1ou# Qyu$O$d\ak6d 8JX |  <\5 _ si <2FcVLSGsT&AL]Z8z:#?Hd>pM."Yd6.9+S>G2JZDQJxkGVB59Z Vf&o?FUGP4.2?.@*)Ag:LkoB"O;9O9^IHgK*C5@#[E8k0Sw@KDfZX;/a9AO]ZL6$Q97/$G*D'hKMmYS20!$ngUmrzRv6iUnP`?B+T,4ABAF<5|-iHW39^S.LY]h1c /)QR)hcZxdD%s:vdWo_HY>mJp*u`,L3I!jEDKEIJ_Q\Jw*J{MpZUgSuf{q@toW} mDdgXQf6GVgD?STzReRdXr4{mhk:HSO/e3Nbdb@r\mhpJxO1Qh# 9*(BwWNhkt}-f~puYeL[<Fo>\9HC]iQ]iYMFq7mYL2[,_cYBk)ZZM<YrpM|c3?3pzM{Sc 0PwN=cK]1`om3ODkyN`mB-@r$<'.*;GTAGGGGGTGGGTTATTCTTTAAAAAATGTGACTGGATAATAAACTATCACATAATACTTTGGCGTATACCCCAACTGATAGAGCGATATCGTTGCAACAATTAGGAACACTACACCATTAAGAGTTTCCTCAGTACCAAAAAGTTCTGACATCTCAGCCGAATTCTCCTTATGTTCATTGAACGCTGTAAAGACAGAAAAAACCAACAAACCAGAACGATTCGAAAGGAGAAGCCAAGATATCCAGAGAGGTGGTAGAGGAGCCCAACCAGATATAGCCCCAGGAGCCTATGACAGAGAACCACACGGAGGAAGGGCCGGCAACCACAAGAATAACTTCAGTGGAAAGTAAGGAACTTGGAATCCATTACCACACTTTCCTACCCTAGGATAAAATTCGAATTTTAACTGCTAATATTTCACCCTTAAACCGTTTTCTCCTACGACTCCAACCGGGTTCTATGCAATCCACTCATCTAGGAGTGTCAGAGCTTGAAAGTCGAGGTATACCCTAAGTTGGGCAACAGTTGACTAGTACTATAGAGCCACATACTCCCAACATAGTCGTTGGGGATGGGATAATTCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:48:28 -04:00AEPt@LAEPt@LAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt,ASPt,B1Pt+B1Pt+BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XSpDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR(P1AMKiIP1RLKdP1WDKmP2AMKw P2BAKK{P2RLKruPBASKKTPBASKKR[PCONKKbhPCONKK`PDMFTPDMFR?PLOCK[PLOCKVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%R SCAN@SMED SMLtSMPLTSPACAemSPACR8SPACAemSVER4.0SVER jSVER,,/Scal@Scan@TUBEE7Tmpr<UserphARphCHRVphDYbigphQLcphTRphTR,8This is the confidence range *ABI_Limits..FVocAEPt@LAEPt@LAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt,ASPt,B1Pt+B1Pt+BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XSpDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR(P1AMKiIP1RLKdP1WDKmP2AMKw P2BAKK{P2RLKruPBASKKTPBASKKR[PCONKKbhPCONKK`PDMFTPDMFR?PLOCK[PLOCKVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%R SCAN@SMED SMLtSMPLTSPACAemSPACR8SPACAemSVER4.0SVER jSVER,,/Scal@Scan@TUBEE7Tmpr<UserphARphCHRVphDYbigphQLcphTRphTR,qJ'8f7}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"CC+MCW5TQJ31N4Vm1l1sNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? 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