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",,III^^^{{{&&&999DDDXXaaavvv      **3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}~|{xvtromljgecaIOOOGHHdde{|   %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a72a59a1e7894f8ea4adbe6bb0823e54Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-Well#K1317_E10_Crusea_calocephala_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zu{&$ 2GVZSF;9E]}Q@vg_f{ a  S . 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B7G)H DI2<M@N$ Q+08=_XV>"3CHGH99G;0K.ZFIL9VIcA VJ > 7QSL ZP%V8 @28P@"U8"a66^aI !`I) W!?2 G%<\ <T64 :\(GE-"M?^.=%'2/bRX%:+3@XA )+[)dDS^Q41RX#B^UR@L IUE`%J$VJ,' C(T`6 F>;:* 5 \/JP_PU F#!!:O_VTc ,#.QUEF` F(&!D[.6>L3NHY,5!X1-A>b!V#HAHK/JPSNL9R/\*J&)LJ4 F^%LTQE(* ZVZ^2cb':!7P@HCSX`I  S2NBP_,&HF1;U( 4& ^&Z &RH%PYMHY(:BS6;aK2H2 01  *"aA eB=Re|j=f4x {Fg =vXzh@7U2|^\bA cz{%!Z!, D]3 #r\f(}HuH&B/P}D5wI6p>}/jDc V|!zdNi nPO, A=jD]Y4='J X vP"_ZX0~~(Bf|PW``<Ys_#NWT%1b5 {m`}? 0PHXM3Dp{*Bxu9[JElyP5n.":AJ`a{"Z9T[EgDM*"B{E1A~wHF#z&Jk>bS*{pb7zff~"%,&r@ .z90l{|7!Im: "D0_:+ ([!tu)^_w>smrK4D=Nf.@*5B;T:z/$"KaDUD6 & %0 9$  P#' 1( $! ( ,W4E 5-8 '!  [F< $%<O&k63G' !H09)DR*# WP>m/- P%S K_?  6F2<<** ""/8 8N K== $1C " "X;7="F[>D7F\>69""|N98=""IL888'""8""""M"G7G}Kp(p7~z*+P !\XpG#=;!z3,}GEy&72fppfnl,B51dYDp&CcR|JS@x% d8,)tcCNc5p$S3LdEWWiMV/J[>"y^@ ^ybJdtHJ%W,I'b)][caz8T,#{?jcK4VUF%68#pk<`5,er=H_F<so.,OL%aH8+_wzn)J5O"74G$*KC=x#dIdE&pppAu8 15Y MN 0 v(3KbPXSsT0njwgEbc?G&SoEj7md+bi:x(pP2P81!/@c 4#V%K-Z3p*I5(AAM7S(2T9I9WGVSdPj9K4;E`FQ{Xk+jlH'(~j"ZP,3aeVRGEBU4WW5&On*o`,x0<pE=Gp4Q:w9*cfdXI:9\9-2hN0KcTN]^T%:3E_QQIROvO>*g_[Dhky]PDd8W8IARUUR-@4lRt4Rf'1).+z7A?)^ZVVPt,J=&4aT@D(0M7~^tKVagT=BGAb|K@6rJ_pKNYFFQ{>i>;9EH8h\G_Fh0G8h^z90tUwh4ItTHH386%D9}QC[YD?iQW8bVb0{Lih:,  qD5hUa^?OM={PF$<dzxVpBp Ahksi= tg;D4)Gdfk%LDe%Bs~C:t[c5 p$MGAAGGGGGTTAGGGTGTTTAGAATACGTCATTGAGAGCACCTAAGCTCATTAATATACTGCCGGATACGGCAACCGATACAGAGACATAGATGGCATCCTTAGAATTAATACTCCAAGCAGTAATTCATCATGATCAACATAATACTTTATAACTTTCGAATCATCTACTTGGACCCAGACCACTATCAAGACAGGATGACATAACAGTCAATTTCCATACTTAAGACCAATCTTTCACATCGAATCAGCTTCTCGCGCAGGATAATCAGCTCCTACATAAGGAGAACAAACCCCTCCACACCCCCCAGGAAAGTCCGCTTCTCATATGAACACCTACCTCCCCCACCAAACAAAAAAAAATCGAATTACAATAGATCTTAGAAAAGGAGAAGAAATTAATAATAATGGATACAATGCTATCATTGCAAAGTTTTCTTCTTGGAATTCAGGTGATAGAGGGTTAGTGGGCCAAGCATGGCGGTCCATAGTTTGGAAGGCCCGGTTAACCTTAATTTAGGTGGACTCGTTCCAACCAAGGTTAAGCACGGCACGAACGGAATCCTCCAGAGATGGAGATAACCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:48:43 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt=ASPt=B1Pt<B1Pt<BCTSj BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA = zTDATA = zDATA = zHDATA = zDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~hLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS=P1AMGTP1RLGOP1WDGXP2AMGaP2BAGGfPP2RLG]4PBASGG?PBASGG=RPCONGGMCPCONGGJPDMFTPDMF=6PLOCGFnPLOCGAPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%=SCAN@SMED SMLtSMPL$$TSPACAi.SPAC=/SPACAi.SVER4.0SVER jSVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCH=MphDYbigphQLcphTRphTR*This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt=ASPt=B1Pt<B1Pt<BCTSj BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA = zTDATA = zDATA = zHDATA = zDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabx{FVocxjFWO_GATCFeat//x;GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~hLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS=P1AMGTP1RLGOP1WDGXP2AMGaP2BAGGfPP2RLG]4PBASGG?PBASGG=RPCONGGMCPCONGGJPDMFTPDMF=6PLOCGFnPLOCGAPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%=SCAN@SMED SMLtSMPL$$TSPACAi.SPAC=/SPACAi.SVER4.0SVER jSVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCH=MphDYbigphQLcphTRphTRV#\*x9*ofW)NK[>9`ht1_n8J{]t>fDb6b@*-D~=7?L+EK|T+1#4v*'bvxZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_?DNchv{"$U]`EzWkp$=RyS4*% `%c~]M:]A|7ZGM9V \+1w.@{Uae[&BTd.u?zcw~CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72We"IgtM,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS`V2]<|':eqB?k3Pmr(7t+= B*Zv4i;mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6LLGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#