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' ! * ,%1!&$*! !  !!  * 43;OE % /6)0<( ! # 5&  $"3)0(+   $ &#   !&'&    "4.   % +C5H59.",,III^^^{{{&&&999DDDXXaaavvv      **3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}~|{xvtromljgecaIOOOGHHdde{|   %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: cfc27d95cf1342ea9f0e67152606aa08Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-Well"K1317_B5_Lobelia_xalapensis_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zmt~ 3>@<3%%Qx+ET]h~Du?nUOsoH<66c i!_@"2DWuA*;MlNy,,/" {T)a8    yk[KBEWrjVLO\puq|3f*p7{p"LRn{vtxrZXbff&y[IJ_|#??zKC_1l l~>?0xH&   /:;3%  )130+''-8CJG:(Av c4!`qCn7 !!      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Bf 82 YT {lrx.>tZ~znwyd.T/\=4O43?ZJQ6-F*9:P2 Ic#"> :  B /5#M0-!H +5"-P5 "b, _$  >-B R4+ ',"D>LAL @%+ I(;  L=d8s"[,51 " 8"""""8 "8P'9r8" -< ""1"8H3"o":""p"jrVy pp8{U!)~|m0}p_ @:d`0r87`8 [m(hJ- #uZ,6'+zX_Xge0/@(-F$YC3g| ~$9svmji(V H/4z@+Swk@&Q.+UV0AKL D Y}R0d%C.E,8GREgc{%?)nd)ZtV8* +11zVL`gw*SpidtQP# OF[a`K~~EF*0s,vIP8]`J 9_3G% zh eFFb '}o!-w%E">24\p|p,i  > > '{ ;h33hDD0w|Wgk]J|BwSan,^K\,Qr$')Xr?S;18D'Q*q9}7n>e?pS6ytrv>$oNZKw1]v8!FIANXUK6q>t<9Y\`:srdu:y`9F/uf4ftL|G{c{0F:X_Dly5fbAxC`lU0Nb]Z|YS..[swLbi[AEyVW\swZ/MxmGZ?7Ze}T6z{Z<GDiU0xYPEH)VZZKU_2OKC9tB&\ we_[>TCx;qfKouzEso2L,`Ea1:1R`wuQ>o:2h2<]Ry4O3ef3TF?iSxp;oYo3WWq.>h2eXijF@mUDj,~`\``=VJ)i|vJ*`op\>+UThWGd3o}_]dp#*X6{U@J:J}U81U^5rkLu'B:<z @&\"\@z8qMGAAAAAAAATGGGTGTTTTCTGACTGACGTGATCATAATATTTAACCTCCTAACACACGGCAGAATACCCCATCGATTATAATGATAGGGTCGCAATCATAAGAACTACTTAACCAGCAAGACCTTCAAAAGGATGAACACCATCTATAAGATACAACTTCTCCTCGACTCGCACCCAGACTAACGTCCCGAACTATCCACCTACGACTCATTATCCCTACGTAAAACTCTTCCATCTCATCCAGTACGGTGTCTGATTACCCAAACTAGCTCCCTCCTATATATCCCAAACACTCTTTCCCAAAGGAGCACGGTCCCATAATGATAACCACACCTTCAAAACAAGGTAAGCCCCCAGAAACCTAGGTCTGCCCAATCTTCATCGCTCACAAAAGCCCATGTCGGCCATCCGAAGTTTCAAAATAGAAAGTTTTTATTATAGGATTCGCGCTTACTCCGATGGGCTGCATCAAGACAGTACAGTTGCCGCTTGGAGTGCTAGTTTAAACCGAAATGTGGGGTACTCTCCTCAACTTACTCTATCACAATATTGCAATGGTCCCTGTTGGGGAGAAATAAATCACATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:48:19 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTSbBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <x:TDATA <x:DATA <x:E:DATA <x:tDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~agLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS5P1AMJLP1RLJH9P1WDJQaP2AMJZP2BAJJ_P2RLJUPBASJJ83PBASJJ5PCONJJEPCONJJCPDMFTPDMF5PLOCJ?PLOCJ:}PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%5SCAN@SMED SMLtSMPL##TSPACAi)LSPAC5SPACAi)LSVER4.0SVER bSVER,,/Scal@Scan@TUBEB5Tmpr<UserphARphCH5phDYbigphQLcphTRphTR,ꁅThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTSbBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <x:TDATA <x:DATA <x:E:DATA <x:tDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabqKFVocq:FWO_GATCFeat//q GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~agLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS5P1AMJLP1RLJH9P1WDJQaP2AMJZP2BAJJ_P2RLJUPBASJJ83PBASJJ5PCONJJEPCONJJCPDMFTPDMF5PLOCJ?PLOCJ:}PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%5SCAN@SMED SMLtSMPL##TSPACAi)LSPAC5SPACAi)LSVER4.0SVER bSVER,,/Scal@Scan@TUBEB5Tmpr<UserphARphCH5phDYbigphQLcphTRphTR<8s*5c'?iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0:_y{F3a.NfL6Obc[yykX 7::Soewo7}:F`5DhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#/D?*Uq$[-o9D4 `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!