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",,III^^^{{{&&&999DDDXXaaavvv      **3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}~|{xvtromljgecaIOOOGHHdde{|   %$$,,,554=<BDDKKKRRRYYY`__gffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2090ed3b98124f8e89b1555226687d73Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_07-00_07433130Seq16805134898_134898mfagan96-WellK1317_B2_Russelia_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zPRSROIFISe|D{2j%X%x0:f+HNHCKgi)Orx\#G b 1Rf&k9 %?[}L*` )'K za; xS3tU5fJ7.0;IXelpniaXOHFHLONHA<>ENTPD5/;`&l[5Eto N PCz<5 (! Buo7 p\"AOJ="jTHA=94,$&,.*# #Dv gZb%{ZA513BfpM7aoI3%      N80o;sa9#          $!;Ek-P1-0/%#08:5-"   B}Ckb.pX" ""  0!:/i4#'$ "&#    :9_Lt2 #"          Q!.8;3Ijvvz- E 6"2    &ocM|j M OR9OQ      Qu :TQj5  'qe@[.>R   K0"ev)     i ++('  CDBG  (emlb$<v6I4>LMd7rM KS1!,v =   1=A-H'} w9im,; 1Y *d7TJO! .T,f.,   PwZK     09vl=)aK`7Q.b}1 (*% %'!  \9DP}G8Z+     #%     k( v@ EF<v& DKK^G1EAgEhv,  "!  &jHA/t6 ekl!h@\p_n+           2b rC!      fI$+   -q<kA       VQZ:Hn!+t3!*!D"Pu2 :d5a ## 'dK+    AwZ0 PEx^t6       _5"Ac  !#  QA[jX]     a0WI*h:5  =o$ G      :kL%            "u2.k"      4+|D:L  $#<mb4$h<f9      >~h0   %)(! $&# D5b!   KI  )NfAEo?#!    ("&^r       1.!az#           <&iHo   (GnO!Er1B    _C x):      "cK>{w1'9 k^)>l) ! 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Qb Ap#p~;GV|pxgp& ppv"& p:-ld,<f;@i8uE$p_k{K: &<edPyB9M7gv/" 2a=Es_([g (![ ma-&<*8HX')U~!4+Q3)-]DA9"3!28M>88'%#34")H-S'%D+% '(HP#A)+@B:+'4Bp# 4&"G5(`!W0= ;'):(<,!2&=>$B)+ $;'5/2,*2b/4,-?45*X*.?8B&2(09/[,&4lT;e9?2G%6RL,E5&-_(DdQ5.(4K#/`;J-gb< 4-&*&N/DB(!$9+=-"&#@4%1>&&-1:D)AHN*!h(7JBD$)J+2(#-J442D1!(3=5;!&:M89s)N/-!&07H0JG?)'@"5\0U=1600M=/&=01H%Z`3#N ^N3*n/WM<>C)1&\2' ' $-hFRY(;,3D{2j60:PI:)$!&1!H5T)O *8$%*?EHG<q`S<8,(8Xw5qBH'HE88)OH<">*?('69/guh\HfRM;z$bLAlDw6HjdiT{jnlk;[69U' OAAGAAAGTGGTTGTTTTAGTACAACGTGATGAGAGCACTAGTCACATAATACACTGCCGTACACGCCAACCGATATACTGGTATCGTTGTCGTAATTCCAAGTACTTCACCAACCAGAGCTTCGTCAGGATTAACAAAAGTCGCTGTAGCAGACCTATCATCTAAATGGGCCAAGACCACTGTATAGAGCGGCTTTCCTTACAAACATGATCGACACGGAAAGAGAAACTACCTCATCGAAGGCGGTTCTTGAGGAACATAACCATATATCCGGTATGTATGATATTCGGTAGATGACCGAGGAGGAAGGGCCAGCAACCACAAGAACACCTACCACGGAAGGGAAGGGGGATGAATCTGAAATCTTCCAACTCTAAATCTAGGACATCATAGGATTTCGACAGCACATAATGCTACGGTTCGAAGATTTTTTTATTAGATTCGAAGCGAGACACAGCTACCGCACCAAGATAGGCGTGTTCCGGTTCGGAATGCCAGGTCAAACCGAGATTAGGGGTTTGCTCTTCAACCTAGGTTAGGACCCGGCCTGAGTGGTCTCCCCAGGGGATTGAATAAAACAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 08:47:51 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt+ASPt+B1Pt*B1Pt*BCTStBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >b|TDATA >b|уDATA >b|NGDATA >b| DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~s)LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AME^P1RLEZ2P1WDEcFP2AMElZP2BAEEpP2RLEgPBASEEJOPBASEEH PCONEEWPCONEEUPDMFTPDMFGPLOCEQPLOCELPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%GSCAN@SMED SMLtSMPLTSPACAgmSPACGSPACAgmSVER4.0SVER tSVER,,/Scal@Scan@TUBEB2Tmpr<UserphARphCHHphDYbigphQLcphTRphTR!9This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt+ASPt+B1Pt*B1Pt*BCTStBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >b|TDATA >b|уDATA >b|NGDATA >b| DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab FVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~s)LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AME^P1RLEZ2P1WDEcFP2AMElZP2BAEEpP2RLEgPBASEEJOPBASEEH PCONEEWPCONEEUPDMFTPDMFGPLOCEQPLOCELPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%GSCAN@SMED SMLtSMPLTSPACAgmSPACGSPACAgmSVER4.0SVER tSVER,,/Scal@Scan@TUBEB2Tmpr<UserphARphCHHphDYbigphQLcphTRphTRA08^#5EN^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]i>\sFbX&r=3zz=Jky0+<:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYX:.<)%,8EE.Om,P>bH6X128)TK7\4v3s'kY6C}B-mq5FaT2V3pVoqCH-p@v\vhsW__Z';fQGE-`x&iJ-,qzjN"o+Ax8amb,.63<%Ri24WF=-U[D~TWY+N@c'v+gK >o>s1H#"$w8@&!]T68n(0Ju*zv&1M2F{H/`WajZUTw|INi@C,6KP8)s5AVihx_7&OMb=#w&bN"@7hhSc%bVm? S8UXabCDkS4NRY0GbR31}ssb[l"G>}O!G,lwdRadJsV&&?-}U`aK|`Vc.O_l;5)F}oP5?z#`D5^jvoy.r%W`MPGh +6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HW.{WctNXbA.PZ>f-6>C#!^Cu_OlYc?pTtfQgvv6=f+wX~FC{[6YN25D0V31K}i>"ILNT,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MSb|TDATA >b|уDATA >b|NGDATA >b| DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab FVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~s)LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AME^P1RLEZ2P1WDEcFP2AMElZP2BAEEpP2RLEgPBASEEJOPBASEEH PCONEEWPCONEEUPDMFTPDMFGPLOCEQPLOCELPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT &RUNT / RUNT RUNT /Rate 0GRunN""JbS/N%GSCAN@SMED SMLtSMPLTSPACAgmSPACGSPACAgmSVER4.0SVER tSVER,,/Scal@Scan@TUBEB2Tmpr<UserphARphCHHphDYbigphQLcphTRphTR