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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 16f61da18349426b90ed22db9ec802c1Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_23-51_06513130Seq16681134623_134623mfagan96-WellK1316_G8_Iresine_diffusa_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z5K^q"M6gC"g~peWAz}dF+ q[J>71$iZL:$  ,CaGs(Q5Or+AIGB=8-S%s)]H5q:uH  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Q-     -VwM, @keRO`g=  ! #((##CloI,     !! $Co '0' `B/$ !$)-/110/./0258;?BCA=82-)'%$"!    2V mH-    >fZ7         1SzrR8%        .RgF-  "##"   1X}V5 6YvuzW8!   +?Zx{bM;,       ;d  uoylO6#  (2?Oe}cF.  )CdqU=)    *KpdD*     #Bid>"   ;^cK9-&! !)./+$            1S\<# $3@GGA8/**,.-)##(+*(%"    .=IPPLD:.$                                                                                                                                                                                                                                                                           /A@^A?AFAEKB.bcpKB_3730_POP7_BDTv3.mobtermGGGGAATATACAGCTCTAGCGGAGTTTTTAGCGGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATACCGGGAGGCGGACCATCGAAAGTTTTAACATACGCAGTAGGGATTCGCAAATCTTCCAGACGTAGAGCACGTAGGGCTTTGAATCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAATGGGTAAGCTACATAAGCAATATATTGATTGTCTTCTCCAGGAACAGGCTCGATTCCATAGCATCGGCCTTTGTAACGATCAAGGCTCGTAAGTCCATCGGTCCATACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCGGCCCCTGCTTCTTCAGGTGGAACTCCAGGTTGAGGAGTTACTCGAAATGCTGCCAAGATATCAGTATCCTTGGTTTCATATTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACATTATAACAGGGGAATATACAGCTCTAGCGGAGTTTTTAGCGGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATACCGGGAGGCGGACCATCGAAAGTTTTAACATACGCAGTAGGGATTCGCAAATCTTCCAGACGTAGAGCACGTAGGGCTTTGAATCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAATGGGTAAGCTACATAAGCAATATATTGATTGTCTTCTCCAGGAACAGGCTCGATTCCATAGCATCGGCCTTTGTAACGATCAAGGCTCGTAAGTCCATCGGTCCATACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCGGCCCCTGCTTCTTCAGGTGGAACTCCAGGTTGAGGAGTTACTCGAAATGCTGCCAAGATATCAGTATCCTTGGTTTCATATTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACATTATAACA$.6@LT_it}.<EQ^gu &3=LZer !,9CO]ix"/;DNZgs~ #1?IT^iv ,:EP[hs #1<IV`lx)5AMXeq}    ' 2 = H T ^ j v   % 1 ? L W b n y    ( 4 A M X d p }    * 5 A M Z g s ~  " - 9 D P [ h t &4?JU`mz ,8DR]jv(4AMYeq} #.9DQ^jv(5@LYco{ %0=ITaly ,8DP[gs)5ANYdp~ $1<HUanz "/:GS^iv+6CP\iu ,7DOZgr!.:GS_jx)6$.6@LT_it}.<EQ^gu &3=LZer !,9CO]ix"/;DNZgs~ #1?IT^iv ,:EP[hs #1<IV`lx)5AMXeq}    ' 2 = H T ^ j v   % 1 ? L W b n y    ( 4 A M X d p }    * 5 A M Z g s ~  " - 9 D P [ h t &4?JU`mz ,8DR]jv(4AMYeq} #.9DQ^jv(5@LYco{ %0=ITaly ,8DP[gs)5ANYdp~ $1<HUanz "/:GS^iv+6CP\iu ,7DOZgr!.:GS_jx)6     2,!44#, )#/#.. ''**!!**8>68  #"8 ";';+-8;;;3  18+"$  3+8+11>;.111). '.'.+ ;>8.+3>+"+;+,""-8($"-"&'3;;;++""+%+;+'+".;;;;22'+3131'+'"'113>+33;8>>>;388188383;33+38;;>>>;8>..;>3;;;8283-+..2$-&;"-2+.3;;;;>2+--328'383+8+'-+282++3;>+222"2-+>;;8;8,8+3>3;;;383>>8;;,8>-+333"&",;+++3;3>;3'3,338>8,+8888,/&83>888*3>+>8>88888!3>'&&8/88&8+88>8888,+4+>44644.46642./&+###%" !4:66*6/(40644*%*04444*0#444/4444+/42/'/"(! "%.       2,!44#, )#/#.. ''**!!**8>68  #"8 ";';+-8;;;3  18+"$  3+8+11>;.111). '.'.+ ;>8.+3>+"+;+,""-8($"-"&'3;;;++""+%+;+'+".;;;;22'+3131'+'"'113>+33;8>>>;388188383;33+38;;>>>;8>..;>3;;;8283-+..2$-&;"-2+.3;;;;>2+--328'383+8+'-+282++3;>+222"2-+>;;8;8,8+3>3;;;383>>8;;,8>-+333"&",;+++3;3>;3'3,338>8,+8888,/&83>888*3>+>8>88888!3>'&&8/88&8+88>8888,+4+>44644.46642./&+###%" !4:66*6/(40644*%*04444*0#444/4444+/42/'/"(! "%.  OP0/ &W ?bcbcQ9_[ZB' `GIP)'4+WU:EB+G -492 )=CC.+ACH&\00RC>11?\E' O\6BVZ Q: Dc]V,a2-SAZR[!V 2X918\[dP\&V/%; ?LG Fb*^ & c bIU[TKDa<@0Q+";=_=&[ A )*KK *:9F46=$ XMN`% N%2YO#482I!( =N%I8D -2 #(4=9<T  $6Oc JI#[[2[XH!UH OI#C,b> WK9G18X_(><8^&:TK 8[33L4\$ 3d8$TF>) < K9H4 DWA%(HI 8!4;-5>DU*:%[*I^</0CL47#\ R2W $3/QVFP+00-.Z+0MZ #VaJ73@2"V$D@^??*]2 8O*;a7+9J`G=Z B#9 ?J7"H B5@)FF-Q]6G@[ZDB]WCB,.VSb[5"+PY6;T;? #Z) 8GA$FKO"p?AF  S* ho.l+j,\0E 2/-8 o7 EIoxYp M2I/.oMQ <v1#%9L/Q TW>{3Rr95?Fn T.F6@]8q]]\+e2: ePC( (wB{OMcd#mkyN`/M#=C,yG`@#M1k#0 &5x QCy1^7wr\.8P7#FxfA/|;J)l>f}Wa~)_@1 rId*>QtX |P@v5#$q 5 ; ?G -NE0mX pQe0RnV qb-( &XX n o3{YbV{!*QDk4$Dr{m\"KW(Ia{$wW2z$bV{onqa QBus.=UW' Nj hE@P"$~0!|_3CEuuo]PN_nCS;+}(H:NO"$,+;#$(4#* (  ) ! !  !-%5   ~3    : "  '    0  #  & *-/  $* "  "  %: &63 6*"1e""&7?#8?#"B827,&@.P6 I?G )?-S>B8,K)m#1?4G0!(CP&,MYKK%D+%zBBPES4>+5+91f,,j^I:(\'R:E(~e;P}]Y333<3<<<BD3:_ri&_pgI/|p#(I3@Y3 wZZ&;5j`Ld,j\Mp,"88BDu8zGPXfIZGyc8pc+aX i} gdhMB$ U)4h,,JJBdW_ 3A_8v,C96gz8?[6Rfpd) Bp k^Saq,f~$G8rI5?@nD_L|p=,G%p#Y*uQPSy%sSnD#;|e_V}0gp p'\[ aN meLG@V[L'c1':0{}DWTq"Xy}~<)! KokKs`cR[B >F K}w6GF?9_3a [Bx~qEBn#ps5_X:f-7H6aHK@B-YYk(GbwiFbJs9B\lQC,JgRumS$oN|O?JW;VB<pv{y=EWayJe*8?S'1@BE@U^Ufvf"(^?F]DX_,aZS9DarcBhX]oNUL-n.@e1SL<Wdwo5R_bv9qIQ+nkR7}4eG\hzeW9PC8vkirK'/EZJTKRZD7+J9=u]K3@6xx@tAP{xkWE$,J%^P\T66;gtPYpW?i3qu$UG2Gc.iLYH63_[k{gDp@.yZLT9.]j1Muc,Xm`]>l +'y9w}nP/U7CAAAGGATATAGATCTAGCGCTGAGGAAATAGTATACCATTGCCCAATTTGCATCCTCACACCGAACCAGAATATACGGACCTTGGTGGAGGAAATCGAGGAAATCACCAACCTGGATTTCCACCGGACGAAGCGTGGGCCGGCGTAAGTTGCAAATCCTCTTCTGGCCTATAGACAGCTAGGTTGGCCTGGGGTCCCAAGGGGCTTGGTTGGTTTCAAAGGCGGTTGCCAGGGAATCGCCCCTGCCTCCGGGGGGAAAAATTAATAGTTTGCCTTCGTAGCTCTCCCATTGGACTCCTATGGAAGGGGCTGGGTTAATACCGTGGCTCCTTCCATGGGAAGGAGTAAGATTGGGTATTCAGCCCTACCTGGTCCAAGGCCCGGAAGAATATGAGAATCCACCGGGGAAGCCAGATCTTGCTAAGGAAGCCCGTATTTTTGGTTTTTTGGTTAAAGTTAATGCATCCAGGTAGTCCGGTTCCCTGGGGGGGGGGGTAGAAGATCCCCAAGTGGGGTGTTAAGCCCTACAACAAGGGCGGGAACGGCTGGTTGGATTGAGTGCGGGAAAAGATAGAATATCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 01:39:04 -04:00AEPt=tAEPt=tAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtnASPtnB1PtmB1PtmBCTSzBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?~4TDATA ?~4DATA ?~4Q!DATA ?~4UDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~xLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMP1AMFdP1RLF_P1WDFi P2AMFr$P2BAFFvP2RLFmPBASFFP PBASFFMPCONFF]PCONFF[hPDMFTPDMFMPLOCFVPLOCFRPPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%MSCAN@SMED SMLtSMPL TySPACA[PSPACMSPACA[PSVER4.0SVER ztSVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHMphDYbigphQLcphTRphTR$)This is the confidence range *ABI_Limits..FVocAEPt=tAEPt=tAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtnASPtnB1PtmB1PtmBCTSzBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?~4TDATA ?~4DATA ?~4Q!DATA ?~4UDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~xLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMP1AMFdP1RLF_P1WDFi P2AMFr$P2BAFFvP2RLFmPBASFFP PBASFFMPCONFF]PCONFF[hPDMFTPDMFMPLOCFVPLOCFRPPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%MSCAN@SMED SMLtSMPL TySPACA[PSPACMSPACA[PSVER4.0SVER ztSVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHMphDYbigphQLcphTRphTRs)B23Hj.L3`|z&mm,_?DB~P^lQbaeX7SoR:+:j,$}<7-? |EXoW3Y~+ctPp%!++(aQQ*G]mysG(B-O*@NzRfu4WyYZ(Wa:nuXBT]qK_^_<~|Rs5AVih5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9rgzE<=#d1a-c_[O~}4}ceGf+Ko[!AETRF[+]18`F7IOoUVn"eHts'MP(D`~<)l47y!R}*D]d0oA{Qk@ /kXSE@a!)DS *n&-HOnO@5}"a'3.5s\T7}{W_z>FfM~V0at'@'|2R(,ul#wT[1R7R>(hZ$~I!|spN$t.D~F45$\j|KA[Lz2fJq3IxdO#rTi7BQr?~Dkfx'.w%`[K{[5I?-Cn#~3~^x??US]Q#V>Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&05t9z"H~:HziP""#]eKZ}X'oM9NB [.MV?^GR(f]/j+>4,"KLh]$X>VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f e7{P`+vr4nCe|vH[p!hjqf[MH-YtDSYcJ|_nO*rSW,S:W1~Uoe`D#Lr4.bX$ss^JXrW1_rP3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S