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***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=TTTMMMlll&&&000999BAAKJJSSS[ZZbbbjjirrqxxx~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e48bc1c57e2742fbb0e55fe5628eb8beSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_23-51_06513130Seq16681134623_134623mfagan96-Well$K1316_E10_Syngonium_neglectum_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z$&+4ARcnsuutsqppsvz|ztj]OA5+# zww{~zwvw|  3G]y@L*y6UcaS;c 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AUIL )0SLo}uX8k>,W%k"e7(O<d(ydXHsp8'Tpdasi6!bwh\8)4S 4GpS uk, JVFOMS<ypuxa8BfPkpJ)8Rd&B~-ed` i|yO,,#%} +pO4,_%9z+i7lCaR+Ye2fJc KMM 3;HIGh,UlSt4$8.49&1Q9/0EW2$V4%.`n:" &2!8s#>)ZLQ@70MTna Y9P6!Y(i7q&& 3J=C:?6tTF' N>k3vNQ49E1LNXkJBJFUXeX41<|'4P(D F-ijoGJjN&D[VJ\[MP1BWC#8BSg <?SG8F/ni0OG_HKG-b>QAt\7I?SE"2/M-5IMBS?:cJ'AN0=-@N^h38[0[wDw==c$N39Y4,,}fX\N?R<3:NY=?);c3_O5$PBT:4/yU8a40"A)b>eC44X9ZV1RJ;^07T ?dM*F9;B18+Mt-Q(1_NPoN,JZP;0K@!fWt%7*0R>=I6nt_%VJ@A1]58&YDW*-XZ(x8] 8~0{I~QkY]KVKPJ.l[;zPJ1$2 }]d7DDs3:_)416"QqwFT C:)5pfVj[D'Nw0cp.@-kgt!_} pP@4AACAAATAGTTCGCCACATACTCATAGTATATCATTACCAAATTTGCATCATTACACTGAACCCGAAATAGTCAACATTCAACAACCAAATCGAGAAAACCACCACTAGGGAGTTCCGCCGAAAAAACTGAAACAGCGGGGGCTTCCGAAATCCACTCGGGGTCTTTGGACACCGATCTCGGCCCAGGGACTGAACGGACTTGATTGCTATGAAAGGCGGTGGTACCACAATGAGCCTGTCCTCGGGGGGGAAAAACCATATATTTGCTATGTTGCCTACCCCCTTAAACTTCTAGGGAAGGGGTCGGTTTCCAACATTTTCACCTTCAATGGTGAGAATGTGTGGTGGTTCAACGGTCCGGCAGGCTCAACGGCTAGGGGATATACGATACTCTGCTGGTTATTCTAAAACTTCCAAAGGTCGACCCAAGGGTTACGTTTTTGATAGAATTTAATCCAACGAGATAGGTCGCTCCCTAGGAGGGTAGGCAATAATACCGGCAGTGGGGATATTCGCAATGACTTACGGACTGGAAACTTCTGAAAGGCTACGCGGTGGGTGAAAAGATTACTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 01:39:22 -04:00AEPt=mAEPt=mAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtuASPtuB1PttB1PttBCTSyBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?~TDATA ?~ҾDATA ?~PDATA ?~ξDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISLP1AM>|ceP1RL>|^P1WD>|gP2AM>|pP2BA>>uUP2RL>|l]PBAS>>O7PBAS>>LPCON>>\PCON>>ZmPDMFTPDMFLPLOC>|UPLOC>|QuPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%LSCAN@SMED SMLtSMPL%%TySPACA\XSPACLSPACA\XSVER4.0SVER ySVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHLphDYbigphQLcphTRphTR8This is the confidence range *ABI_Limits..FVocAEPt=mAEPt=mAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtuASPtuB1PttB1PttBCTSyBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?~TDATA ?~ҾDATA ?~PDATA ?~ξDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabwFVocfFWO_GATCFeat//7GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISLP1AM>|ceP1RL>|^P1WD>|gP2AM>|pP2BA>>uUP2RL>|l]PBAS>>O7PBAS>>LPCON>>\PCON>>ZmPDMFTPDMFLPLOC>|UPLOC>|QuPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%LSCAN@SMED SMLtSMPL%%TySPACA\XSPACLSPACA\XSVER4.0SVER ySVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHLphDYbigphQLcphTRphTRa'gi8jOd_C26R&w^/~!KpmvwM,h_B#RfJ}^m7Wodb8a!l`s[C!/.wC#xh)fVzMVlYXjld96"$i$/V5Z=(^VmTc T-*6?|$lQ%e+Bw9#MBz3)-fA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]>R/Ury&E#theXciW 45=)ySQ}3P?G5g:e:KJl{X\^M1}Gk&0 1GG&iw.P&-Gt?E>@d&r&iox~*4fC\*WiEa1i`\z~0+'HT]`Dod&}JN,C`hc31K}i>ZIgtM,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS`V2]<|':eqB?kgP<((7!+43B4Kv3z.mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6LK-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]w|ceP1RL>|^P1WD>|gP2AM>|pP2BA>>uUP2RL>|l]PBAS>>O7PBAS>>LPCON>>\PCON>>ZmPDMFTPDMFLPLOC>|UPLOC>|QuPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%LSCAN@SMED SMLtSMPL%%TySPACA\XSPACLSPACA\XSVER4.0SVER ySVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHLphDYbigphQLcphTRphTR