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'S~\?0" &(OTWdP5% 'nfRB0   d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=TTTMMMlll&&&000999BAAKJJSSS[ZZbbbjjirrqxxx~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a04381ab80814591bea03a9c404172e9Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_23-51_06513130Seq16681134623_134623mfagan96-Well K1316_C1_Piper_liebmannii_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z2CLNQXew "18988<FQ\a`XK>3,(&# {mc``a`[QD4#    %.1/)  (09CN\mDw 8_m"d9m/q_PEABFLPRQONORW\`ccbabgp~*BZq{dJ.HV+><F3d1g t; "=Vj{ vR0lVB2& !'-24679;?ENZk 0_3NWQB,^ }\G>?J^|% KhY'5Ma8qS?2+'(,145436>L^p|sfUB0!  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X!0Z#,^a-@0;M.Z' ODCVA^T,POJ1cTZNX%AS.DU SVc$Z68_c;2HI4ZbXPC=^7Oda"J3B?Xc5?X86T/VI= ]FZ;HW\B !Q0U7)W./ 0U $U  OB/[CH.]:HQ>9PUX?GEI!6X$C 0M#O(Q=S-&?@1?WC`] #):';_C  y9<VCF3}W&I0~.<j|H".hH.0Y -;~sW sKlsXw:YV =\@iD_R !K{I {-eRa xKXGZMb~]=(&/t8M}A5$i9k4R<YD4]wLWI42Ci  9C,~<H p X?tv bn!Z*z${:$->V-Ht2'^OG0}q }Smzwz+88M. ER7O!B+Bh*^OM|uz0HL ^a#*}($4d|,&GpY/[E|6KZb}{Nh=`zr^68$ |4 iSDQKzuVWI+%[fo~Yq]NN0TV6CJ65*2+>=*C%;+%/*1@50!/ &     .     2             (   & %+ 1 6 -1*0:#9&&%_6/% >0&2$ ,-3"#A#K///19!d:#@'58I//<>J;KAo23S>N!?3L<(F( %M 3)&&#,G$K/0%\%%'L//////,%p2'ppu8PO_pW-8g}NZ#O1kK:xPo.Hyy8vy.q 5I |yH_:+qv'>8K,sP},][#nUoCDV2+}N>W W/SW9JFEiK: 0+{Yp =V 9G?dYLs&`=boAvU%4X8oPR]D,P ,nJxDiIy1kT8WQ4: ))*B1{D0-p,8/pp6qZrfp {VKe."SOS*d5 eRP4tFrhOdH^7P9cd w Y{KGveM9M4yBms_<5C+PN=K,8_UT'a()5u?a)"1Es9c&/A^JJ(77B2?*oIbT{TO38FcZqlTC.aAB.SPF6:MC'Ltq4$Ru-xV_R";YB(_09,!b*|c>`B8aO/.JFJ1e=CB;8I+28-GX5%:.*9j<34@M<`IV`ZWi*P8lL%16)=2$?!1YYC*>/J&489J*!2>"WgBYk~QmtFQCZkb>7Z67vDMpATC,7$3h;;YYl7hvHfl1vQyHK%E5JTSuP/EO(iN?jX*[HB&>8w)/T2=Z~4`F9q?+l;B{)R6!6.)z*FG>6>R61<+Px2x(]*?'`>J0>K1y{(rB34Zs>9P.(|O;=+Ln<h@8KDE@>M!HPvFRM9wpSidNTA-Y1R27@'G>iNV-;0Y9`CA&p]+.Ll`0>SN8 9`T~bMg:+!S.95h/:?D%m H,U~<7Lo0ZGAAAAAAATTCACTCTACAGCAGAGCTCAGAATATATTATGACCAGATTAGCATCATAACACCGAACCCGAAATCACGGTCCTTGGAGCGGCAGACCGAGCACATCACCACCCCGGAGTTCCCCCCAGGAAGCGAAGGGCGCGGGTAACTAGGAAATCCTCCCCGGGTCACGAGACACCTAGCGCGCCCAGGGGGCTTAACCGCTTTGGTTGGTTCCAAAGGCGGTGGCATGACAATGAGCCCGGCGTCGGGGGGGAAGAATCGAAAGTTTGCTTCGGTGGCTATACCGTTAAACTCCTCTGCAGGGGGCAGCGTTAAGAAGTTGCCTCCTTCCGTGGGAAGAACTAAGGTTGGGTTTTCAGCCGTGCGCGCACCTGGTCAAATAACAATAGGATAATTTGGATGTTTAGCAGTTTTCTGGTTATTCAGAAGTATTTTTTGATAGTTTAATAATAATTAATCTGACCAGATATCCCCATCCCTAGGTTGGGGGTTCAGAGTATCCGGAATTGGGATGTTATCCATGACAACCGGGATTGGTGCCACACAAGGGCTAAGGTGCGGGAGAAGAGAGAACTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 01:38:24 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TTDATA ?TDATA ?TSbDATA ?TҶDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~}QLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMDhP1RLDdeP1WDDmuP2AMDvP2BADD{ P2RLDqPBASDDTPBASDDREPCONDDb!PCONDD_PDMFTPDMFR)PLOCD[UPLOCDVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%R SCAN@SMED SMLtSMPL!!TySPACA]/SPACR"SPACA]/SVER4.0SVER ~SVER,,/Scal@Scan@TUBEC1Tmpr<UserphARphCHR@phDYbigphQLcphTRphTR <This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TTDATA ?TDATA ?TSbDATA ?TҶDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab5FVoc$FWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~}QLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMDhP1RLDdeP1WDDmuP2AMDvP2BADD{ P2RLDqPBASDDTPBASDDREPCONDDb!PCONDD_PDMFTPDMFR)PLOCD[UPLOCDVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%R SCAN@SMED SMLtSMPL!!TySPACA]/SPACR"SPACA]/SVER4.0SVER ~SVER,,/Scal@Scan@TUBEC1Tmpr<UserphARphCHR@phDYbigphQLcphTRphTRV#\*x9*eofW)NK[>9`ht1_n8J{]t>fDb6b@*-D~=7?L+EK|T+1#4v*'bvxZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_?DNchv{"$U]`EzWkp$=RyS4*% `%c~]M:]A|7ZGM9V \+1w.@{Uae[&BTd.u?zcw~CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72WeS[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0oW~!Te4;,: TVc:B?tVbmL\Ht+d,Ax_rp$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VD*p;I:/wEkfptir"8|oTq}A|1D@<7>{AXG9{$5t{cQ&=#pmei;EmEH[L7+C%W|YYi]yFy'SKbvUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>UbeP *"Y3hk?^NHE,v*(]BcYAFc=gMAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTHB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TTDATA ?TDATA ?TSbDATA ?TҶDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab5FVoc$FWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~}QLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMDhP1RLDdeP1WDDmuP2AMDvP2BADD{ P2RLDqPBASDDTPBASDDREPCONDDb!PCONDD_PDMFTPDMFR)PLOCD[UPLOCDVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%R SCAN@SMED SMLtSMPL!!TySPACA]/SPACR"SPACA]/SVER4.0SVER ~SVER,,/Scal@Scan@TUBEC1Tmpr<UserphARphCHR@phDYbigphQLcphTRphTR