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8[sxs|V46 "17DVR[9*'   &%%! =O^aqiN5% $-,-).&>(:TyyyMAUJ7GZzpjdP,      $;BB2) &+B15! !;ASHH-%$6,/  &#       7/C6dc[rgJ,.5*4?GbpfstdP0(-)#3#      )&)1545    &DAWrrs?M48*24Edco\FH&&  ,#2F`a[:  !,. *   /863B?(!HPnde07   GOo[k]N:!  ,P5EA' &/3$.*+F5 )..$ !1JIEY`M)  2EKV66     @?Q-  $/8W953 %&)         &"        !    #   )   '#    d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=TTTMMMlll&&&000999BAAKJJSSS[ZZbbbjjirrqxxx~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0e47ff00cc7d48ec99cae22dfac93b36Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_23-51_06513130Seq16681134623_134623mfagan96-Well&K1316_A4_Rhynchoglossum_azureum_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z %Jp6xYxdazFnU<'    {bUWduxdJ/vmx,=5N!IXSC3),>`J{5nAGC~I}dVRTURJ>65=M]hljfehotsgR8  +5=GXrBx;{'D_t}rQw"s\SOOPT`uAiX0 %3?VLiP~Fkm3wO52B_!LyK[6{`OFDEGKNOK@/ '5?DFHJMOOONOS]jz*qCk]XLCQ=pm w( L 6 .]=8;:09[n}th\RLLS_lwyrcRE@CHJD6"    !K =_jbM1bE,   &6?>:;Ga#wbvJtf!*D/6.Mt~v^]gqvvrlfa]YXZ_ejkf^XXbv`A&  (5DS^fjnuZas1dA59I\krof]XZbjkcQ9!   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(w@ecL<Kl*dd*u]/wa8{q'4p;8ffR}j ,u p!Y}"UXCT-v  +`Nkp< Wr%tMG&Bsb@Bcp9s5LzjpzSo<8Bp;'<6;"cFRP xdLb}>;#A;Kz:TK6{vuf\( +AZub">Esr764}C1xd-E)7FpEqD # <p  o\KuJ>#=!e13UT~[ 4?A/~WD;Q'bk+A&yl0.5EOqesrt-[c k x3H]ugwO ;^~1_{@>(r1,!*()=5?/cJ~!U `~,nX2QKF+!MARG}b+*x'bWkhe23}0k}S1>{  djhZ^IriMk\O\5{/'ZE2Foud\EW< c2IDJcUtdv!0s l j<SRT!ml92Xm.sY)OND o{^,{db4EdQi"Li8\wTa*(jTGACATTTATAAACCTCTAAGACGTAGTCAAATCGAATACCATTGCCCAATTTGCATTATTACTCCGAACCAGAGAAGGCGGTCCCTCGTTCGATAAACCCCGCACACTCCCAAGGGGGCGTTCCACCGGTAGAAGGGAGGGCCGTGGTTTACGGGCAATCCTCTCACGGCCTATCGAAACACATGGCGGACTTGGGTCCCAACCGCCTTACCTGCCATCGAAGGAGGCGGCACGGCAACGAGACCGGCCTCGGGCGGGCCAGATCGATTGGCCTGCTCACCTGCTCTCCACTTATATTCCTCTGCACGGGAACGGGCTCCAACTCTTCCTGTTTCCCTGTGACGAATTTCCATAAGGTAAACCGGAGCGGCTCGATCCAATTGACCGAGAATCGTTGGAAAGTTCTGTCAAGGCCTATACGTCCGCGTCAAACCCCCGTGTTTGGTTTTTAGCCAAAAGTCCAACAGATCGGCTAAAGCACTGCCCTCCGTGGTAGTGAAGGATTTCCCTATTCGCCCACTTAACACAATCTTACACCCCCAGAATGGAACACTCGCCCGACTTAGGTAAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 01:38:44 -04:00AEPt>1AEPt>1APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTS~pBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?^TDATA ?^DATA ?^S|DATA ?^DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR@P1AM<xhP1RL<xdSP1WD<xmCP2AM<xv3P2BA<<zP2RL<xqPBAS<<TPBAS<<RsPCON<<bPCON<<_PDMFTPDMFRWPLOC<x[cPLOC<xVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%R8SCAN@SMED SMLtSMPL''TySPACA[SSPACRPSPACA[SSVER4.0SVER ~eSVER,,/Scal@Scan@TUBEA4Tmpr<UserphARphCHRnphDYbigphQLcphTRphTRlThis is the confidence range *ABI_Limits..FVocAEPt>1AEPt>1APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTS~pBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?^TDATA ?^DATA ?^S|DATA ?^DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR@P1AM<xhP1RL<xdSP1WD<xmCP2AM<xv3P2BA<<zP2RL<xqPBAS<<TPBAS<<RsPCON<<bPCON<<_PDMFTPDMFRWPLOC<x[cPLOC<xVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%R8SCAN@SMED SMLtSMPL''TySPACA[SSPACRPSPACA[SSVER4.0SVER ~eSVER,,/Scal@Scan@TUBEA4Tmpr<UserphARphCHRnphDYbigphQLcphTRphTRMKdkij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9HbM|(%: h4qKl}M2":Da^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9kf'zbO\8b790-I;dfAY(y'tMz/yxf/5f0]/joM`)6-_X.> %9WK] O7e<>/D?*&qDp-o+9mW'cj!Q4sIRD8z{q>t[XAn@xg-W!i8- rwp^H8:KbXEKz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAlI{_*.9nWFa-st2hSXdF#lQ%cy-*7y 4/a"XNL3`='p]gQ-Po@EGrAxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnP_I[Dd@2g|5J_@A[]"p64y^M_9ni|HZ/UNl*]Pz%y;|>EYWC.%o,n`!3#C,F2id7-J!."^O[,z^E$6d+XnhcjPGh{;qeXd9U|U0xm$dp"&`#Ya]Z0'c'xo "*b2(sG,XQ9P9zMC`+MTk0WC.%o,n`!3CS]n?KGxDIBG(XX7&ZoMQR,9hLAEPt>1AEPt>1APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcAUDTB1PtbB1PtbBCTS~pBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?^TDATA ?^DATA ?^S|DATA ?^DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR@P1AM<xhP1RL<xdSP1WD<xmCP2AM<xv3P2BA<<zP2RL<xqPBAS<<TPBAS<<RsPCON<<bPCON<<_PDMFTPDMFRWPLOC<x[cPLOC<xVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 3RUNT &RUNT  1RUNT % Rate 0?RunN""JZS/N%R8SCAN@SMED SMLtSMPL''TySPACA[SSPACRPSPACA[SSVER4.0SVER ~eSVER,,/Scal@Scan@TUBEA4Tmpr<UserphARphCHRnphDYbigphQLcphTRphTR