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***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRTTTMMMlll&&&000999BABJJJSSR[[Zccbjjiqqqxxw~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 218fc717fb8e46bd978a36816174d49bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_22-03_06503130Seq16680134621_134621mfagan96-Well$K1316_H5_Rhynchospora_ciliata_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z &Hh!<Vq2Le| $,9I\p}||\.Z2 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$         2    K                6 ! # #%$93-$.!$  &" $4"(!=,2/..')' - % &0+<$1@!$(!+.)20($8 .6,-09<<:0DE?ACF2 K=9U8;?QR%G-?#?=)!H>MW5J(p:?Q30//00&/&&&/&/_&u,d~,N(kp&6p2p .-8i8E,Ve_&< opZN,K$.wvpcmszydOm,Y0y.DyWSEnd~SwS(8Z=7}d*w1tp8G<=Wfzv\IH)&*Ep_yi-pX8,!",JB~aj6xJ{pKN8d\ pBnppFs8ppV[ppzPp]  "Ud$I':'$m,`W,XjD'}phY3XR, H1mw#?@ 1HZccro+(7 '%41#!$#1  @,#5$(S%* 3!1""?1#$/$-(K)3)-: 1(3(&!*"-6=# $*&05)'1&+/(&A/=76//Q@!)4!H&-(/($+'0,=,A%+"H`2;! *$+ !!2*7,#",8G]"-"%*+ '(!(8#""%<*'(!)1J*".-=1F2( &TE2 (!3,"!*<$4$!:!2"-D!&(8*#WC7(6;3#X8$)/(&!:9(M?=%=A1+-,3 -C24;V22#+%(%t 'T)$,%#)").![6"E$57]O5:>+0##7#/6R@' "/. #- A'$`i;VB.8_)BOC+<(!`Mh4=1.<6/"Ub/C,*E-CW=Z} lJG8GAAGAAGTGGTTATTCTCCGAGGTTGTGATTGACATATTTACCGTAATAAGGTTCAGAACAATGCCCATTAGGAGAACGTGAGATGGTTTCTTTAAATCGCACCACTTCGGGGAGCCGTGCTACAAGTTCAGGAAGAATTTGCGCTAGAGCCGAAGGAACCTCCAAATCCACAAACATTTAAGAGCCGAGAGATTGAACCTACATTCGTTAGCGACAGGGAAGGATAATCGGTTAGAAAGAGACCGGTGGTAGAGGAGGCTAAGGGGGTGGTGAAGAAAGATAATTTTTAGTTTTTCACCCAGGAGGAAGGGCCGATTCCCATATAACCTCCTTTGGAAGCAATTAAGGGGGTAAACTCCGTTGGCCTCACTCTCGTCCAGATGAATAAAAAAGGATTTGTTGCTAATATTAGCCCTGCCTTCGCAGGTAGTATTGCAGTGTTTTTAATTACTGATACTGAAGCCAGGTATTCCGGCCTTTTGGGGTCAGAAGGAAGAATTTTTATCTACCCTTAATCTTCTATGAGCAAGTCTGTCGATGCAAATAAGGGTGTACTCCCAGAAGTAGTATATTTATAATCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-23 23:51:03 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtwASPtwB1PtvB1PtvBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?TTDATA ?TDATA ?TS>DATA ?TҒDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE!LAST~~}fLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMGhP1RLGdYP1WDGmuP2AMGvP2BAGG{P2RLGrPBASGGThPBASGGR!PCONGGbPCONGG_PDMFTvPDMFRPLOCG[=PLOCGVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL%%TQSPACA]BSPACQSPACA]BSVER4.0SVER ~SVER,,/Scal@Scan@TUBEH5Tmpr<UserphARphCHRphDYbigphQLcphTRphTR=This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtwASPtwB1PtvB1PtvBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?TTDATA ?TDATA ?TS>DATA ?TҒDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabJFVoc9FWO_GATCFeat// GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE!LAST~~}fLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMGhP1RLGdYP1WDGmuP2AMGvP2BAGG{P2RLGrPBASGGThPBASGGR!PCONGGbPCONGG_PDMFTvPDMFRPLOCG[=PLOCGVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL%%TQSPACA]BSPACQSPACA]BSVER4.0SVER ~SVER,,/Scal@Scan@TUBEH5Tmpr<UserphARphCHRphDYbigphQLcphTRphTRITNn%&nJ9."G>H15KK;x^OSvqy4=(Wz`&t$SUf^3i&6j|z[vRP|ZzrA^iaHtV2}2?Yk6]DZSPW~VlWj+8w*<_swW^$oVv+rrbN<6h8b`[.b;$,PCH-p9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YJ_93dy5*=gb_nwkI6dNNwM@B8{A)P|p$S!T,lwd-l#fr2i:}WL~?O./bE:#&PV)ob&6O`Z?5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9n5N`!1;ULWyS#|Y;;y+Y3y'EV}sRCkSh!0i9>Yn2Pajeo4]tRi^as>0cPX*F8Bir!3EY$9eKoQD5pYHT"Hf.Q*s`AtI_h`"9P39-;mb8p]U3~b2(G!?zq:w,:wHwARnl(}wp9Q9u79"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\f. uXy00?|CTVN6mYt5D7z]OA[.=*QepR}W;(Ou]9g~~hY8|kb+N4+0Oj!Q4M"}JMzR9vAMV^@E P:i~8m+adJsOlG6]}fWG\|J_b.O_l;,[[`7VAd#RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziP-2ZE<}Q6odPz%X;Frxp/YgnDATA ?TҒDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabJFVoc9FWO_GATCFeat// GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE!LAST~~}fLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMGhP1RLGdYP1WDGmuP2AMGvP2BAGG{P2RLGrPBASGGThPBASGGR!PCONGGbPCONGG_PDMFTvPDMFRPLOCG[=PLOCGVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL%%TQSPACA]BSPACQSPACA]BSVER4.0SVER ~SVER,,/Scal@Scan@TUBEH5Tmpr<UserphARphCHRphDYbigphQLcphTRphTR