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(Bi$ uF  `pd5!%4OkyB?* )St}eL4/2GSasUK! 7Lf|uP:! .UejM?  0VcqxoU6CT}xg>2 (,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRTTTMMMlll&&&000999BABJJJSSR[[Zccbjjiqqqxxw~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6c2343712b3844ae8b509f725f73c257Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_22-03_06503130Seq16680134621_134621mfagan96-Well!K1316_G11_Indeterminate_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z$2Jn-V}4So(6CNUZ^bjs   tGlH!zutu{/W&?Vq vh[O@-kH pCy\D. 5W{Q!CZeg^K,PS*9`8j/@Rj@-z/",S. 9fKEbz"w(wXGABDD?81-/7AJMKD=9:?EIHD?;<@GMPOLHEB@@@BFLU_jxE$aS k!jQ>/&""$(+-/000/,(##''% !"  #Bo {s]j;   %)++)&!$/;FMPMF<2)!      #_d2$/D $()&"   0ZU"lV"g.          &/8?BA:2)$$',037B^<x1ptV@0%!)4ALRSMC5' &5BKMI?2#=ZBLS,i\  '7EORMB3"     2NDenT3va(f,   'c5/hzC !.:BE@6(    'a"6G%pX*  !W8uZ>t( !!   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"5I\luwqeTB0!    #0>JTYWN@/   &6I]q}lXD1#          bq@A AQ֡AVmKB.bcpKB_3730_POP7_BDTv3.mobtermAGGGGTCCCGGGAATCAGCAGAGCGTTAAGATTACAAATTGACTTATTATACTCCTGACTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCTTCAACTGGTACATGGACAACTGTGTGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGGCGATGCTACCACATCGAGCCTGTTGCTGGAGAAGAAAGTCAATTTATTGCTTATGTAGCTTATCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAATATGTTTACTTCTATTGTGGGTAATGTATTTGGGTTCAAAGCCCTGCGCGCTCTACGTTTGGAAGATCTGCGAATCCCTGTTGCTTATGTTAAAACTTTCCAAGGCCCGCCTCACGGTATCCTTGTTGTGAGAGATAAACTGAACAAGTACGGTCGTCCCCTATTGGGATGTACTATTAAACCTAAATTGGGGTTGTCTGCTAAAAACTACGGTAGAGCGGTTTATGAATGTCTACGTGGTGGACTTGATTTTAATAACAAGGGGTCCCGGGAATCAGCAGAGCGTTAAGATTACAAATTGACTTATTATACTCCTGACTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCTTCAACTGGTACATGGACAACTGTGTGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGGCGATGCTACCACATCGAGCCTGTTGCTGGAGAAGAAAGTCAATTTATTGCTTATGTAGCTTATCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAATATGTTTACTTCTATTGTGGGTAATGTATTTGGGTTCAAAGCCCTGCGCGCTCTACGTTTGGAAGATCTGCGAATCCCTGTTGCTTATGTTAAAACTTTCCAAGGCCCGCCTCACGGTATCCTTGTTGTGAGAGATAAACTGAACAAGTACGGTCGTCCCCTATTGGGATGTACTATTAAACCTAAATTGGGGTTGTCTGCTAAAAACTACGGTAGAGCGGTTTATGAATGTCTACGTGGTGGACTTGATTTTAATAACA(7@HQXeo{ $5BSam{#.9HR^iu%0;EQ]jx %2=HT`kx "-8CO\jv+6AMXdq|)6BOZfq~ "/>HS_jx  # 1 ; H T _ j v  + 5 @ L X e q }  # . < G T ` l w    * 5 @ K W b n z  , 8 E P [ g s ~ '2=IU`lw)6BNYdq~%2>JUbn{ "/:GS_lw*6CO[hs~&3?JVamy !.:FQ\hu*6ANYdp| %2>JWbnz !/:FS_kv !,9DQ^jv+6CO\ht)5BNZgt~#/(7@HQXeo{ $5BSam{#.9HR^iu%0;EQ]jx %2=HT`kx "-8CO\jv+6AMXdq|)6BOZfq~ "/>HS_jx  # 1 ; H T _ j v  + 5 @ L X e q }  # . < G T ` l w    * 5 @ K W b n z  , 8 E P [ g s ~ '2=IU`lw)6BNYdq~%2>JUbn{ "/:GS_lw*6CO[hs~&3?JVamy !.:FQ\hu*6ANYdp| %2>JWbnz !/:FS_kv !,9DQ^jv+6CO\ht)5BNZgt~#/    $  +35:1 :-4-*64+6::::6)!)*>6>>86)88+*8/8>/>86>>>>>>8>>>>>>>>;;;>>;>>>>>;;>>>>;.;>>>>;>>;;;>;;>;>>>>;>>>>>>>;>;>;>>>>>>>>>>>>;>>;>;>>>>>1 >>>>>;;;;>>;;>;;>;>;;>>>>>;;>>>;;;>>>>>>>>>>>>>>>;>>>;>>>>>>>>>;>>>>;>>>>>>>;/>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>36>>>>>>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>8>8>>>>>>>8"3%)2>>>>>>>>>>>>>>>>>>>>>>>>>3>>>>>>>86+>>>>86>>>>>>>>>>>>>>>>>/>>>>>>>>>/'>>>6>>>>>>>>>>>64    >>/*>>>:::::66:4:::6:6:6164./0::::6:10:6:66:::6:6:3::#:::4:/54443:::-):6::::3:/:0""1+:::3:+:1::33:::::(2:2100)       $  +35:1 :-4-*64+6::::6)!)*>6>>86)88+*8/8>/>86>>>>>>8>>>>>>>>;;;>>;>>>>>;;>>>>;.;>>>>;>>;;;>;;>;>>>>;>>>>>>>;>;>;>>>>>>>>>>>>;>>;>;>>>>>1 >>>>>;;;;>>;;>;;>;>;;>>>>>;;>>>;;;>>>>>>>>>>>>>>>;>>>;>>>>>>>>>;>>>>;>>>>>>>;/>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>36>>>>>>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>8>8>>>>>>>8"3%)2>>>>>>>>>>>>>>>>>>>>>>>>>3>>>>>>>86+>>>>86>>>>>>>>>>>>>>>>>/>>>>>>>>>/'>>>6>>>>>>>>>>>64    >>/*>>>:::::66:4:::6:6:6164./0::::6:10:6:66:::6:6:3::#:::4:/54443:::-):6::::3:/:0""1+:::3:+:1::33:::::(2:2100)   G  =JB[A VWF'4K05!%Z4aK,JGF- 8Z[/%-=1B<-0^4OK>^F8Y4bA!^cL>$65LPc#<` ;?QR Q\`\`0RF 9CFGa!E ,O]=^5U]H =W=/D_58KZ'J[%:b'@7R6cI4/[`YN:&;ZE^=2I? `.@EGE54@C].62(N0M= :TE&.`I-X(3@@):@P0, 9K&#bBVW 8 @S*Q\ @;^>N4NAL%)&]'5(6 c;[ZP\U*% :\5F]H N$NL9, A ?DCa7,:*A2X.Q[*$9 4!XB<^GYY ,^:'_95@I^6VN'D]( ."&2 4QbDY >+L \0FEOX@%<L K[YP%F [ 6 ?9]C$6- W0S!-B_d>?WD -/03%B5-3)LScX"V,D$!L@9 \ ;[M>E)7L2 S&[T&YOCX'BM*;IFH?_G!` <N.W&T9_HE.@D>TE 2`ME@2B*G76eC395gs2y! CZ2FsvGkNMJeCuc]X+`*8Rmf_@`% mbR'CM""mJ&K_N^9tyb'EBi7 UqmmxXhhcQu}Z84m%7I ~B{D?A+|cm!E+D"gG J0IcZOSV_|L0X  w~v<26JV\ye]mM-^"Wqokf!dW?(" i+M &"K5[ K-0Szz>2`Pyk RiH3Y8B ;VUN\92 57TUJUVM!s'&@rv4b~;gRc!t`7v67]BUJ:T+r" Voh1`+ tsd Ic) do }cG`q.|zgjiv[jSQa8VSZICBRO;?KAD/51$'!3'*;,93"'&'# " $                                 3 7      !!  $((% 73#/ #) "./ 6/ '2"(*"2+64 & "2N>.==V=='=EG&.`4.2228:>:A-"4N+/FB4A/D,=YT11*A2QKB.=M4=D5E5[X\E?DCS_c*;3<6FGjLGE8UPK99999#T4WSBnYT2&FZKF0fNLG\DKB68OKUElPCCL9CCCC99C9W9kppp8~wVZzp$qN1.,8DoPY`  3d-_/@A ,:d&>mr`<8dLtp9#& p#tZtp8YVv+OLyp6dxmBuHFSpD1CY*K+8 (7pc@MLFF U ,}ppPbcQ5,{>d,5d9K8!,AT9uBpp@(j#!p/WpY( $ i'p"p\YW]{pg8 ; Fq 4i xWRt$@2/[# q\qaV-e$!*^btIM .r/M2D$&3D'A02,J1+:EJ#3+IL9/ '!3%6) 2!-D! 85 .%"83$ M;+(&!!,(,$"#+&',--.'+)-%)))(/ ;("# *4$5-%.1/*$5;7$'+@)!#),*%""(*''*,#'/6/+=.F 3/)@&+?'&+)!/*.(EK'@;!Z8@+ .(&*E('/!HY-("<;( &?*"$"*.4=H38,A+('3% 2*060GB'D !N3!*C*<4$1O!(8"53%:!Y!?J4[|LOAJBM5$(8)@50O6Q:@Wf"4/021M|6)D^SN;D-7D B: 2,9QCG)T+FKFV7J^U$OFkuNtC,!-+^CGP2#2Za2c!P-?ki R6[GAAAAGGGGTTTTTCTGAACAGAGCAATGATAACACCTAGCCACATAATGTTCATAACAATGCCCCAACGGTTACTGATGGATCGTTTTCGGCCTTAGTATCACTTCTGGGGCCAGAGAGAGTTGTTGTGGAAGATATGGAGATAGAGCTTAAGCAACCTCCGAACGCTCCAAGACCGGAGAGTTCTGAGATTGAACCACGATACGATATCCGTAGGGAAAGCGATTCCTACAGGGCGATTGCGGTTGAAGAGGAGGCTATGGAAATACCTAATCTAGAGACCATTTGAGATTTCCAACAGGAGGAAGGGGAAATAAGGATACACCCACCGAGGGAGCCAAGTGAGCAAAAAAACACCTCGTTCCGCTCGATCGTTAAAAAGGAGAAAAAAGTATTGCCGCCTAAGAAAACCCCTGCATACGCAGTTCTTCTCGCCGTATTAATAGTATTAGATATGGAAAGCACGAATTCCGAACTTTTATAGTAATAAACAGGGATGCATATGTACCCCTGGACTCTTATCGCTAATTCAAGAGATCAGCATAAGTATGTACACCCCGAAGAAATTAAAATCATCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-23 23:51:51 -04:00AEPt?dAEPt?dAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?VTDATA ?VDATA ?VS?DATA ?VҕDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMChP1RLCd>P1WDCmJP2AMCvVP2BACCzP2RLCqPBASCCTiPBASCCR&PCONCCaPCONCC_PDMFTsPDMFR PLOCC[2PLOCCVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL""TQSPACA_SPACRSPACA_SVER4.0SVER ~SVER,,/Scal@Scan@TUBEG11Tmpr<UserphARphCHR!phDYbigphQLcphTRphTR :This is the confidence range *ABI_Limits..FVocAEPt?dAEPt?dAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?VTDATA ?VDATA ?VS?DATA ?VҕDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMChP1RLCd>P1WDCmJP2AMCvVP2BACCzP2RLCqPBASCCTiPBASCCR&PCONCCaPCONCC_PDMFTsPDMFR PLOCC[2PLOCCVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL""TQSPACA_SPACRSPACA_SVER4.0SVER ~SVER,,/Scal@Scan@TUBEG11Tmpr<UserphARphCHR!phDYbigphQLcphTRphTRa,pEnO"|#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? 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