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***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRTTTMMMlll&&&000999BABJJJSSR[[Zccbjjiqqqxxw~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 73a464d06ea742c9a53679bf929daa9dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_22-03_06503130Seq16680134621_134621mfagan96-Well 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S+WnrR=o qwS:{tFquuqidX^[TXRIN9M;5GE07^O=<4?'/1'8!.$"#& *!! ## ,          !               /       DD2%+ '=I /')* 87.&&#I#   - F#)"% +>( M1F =,=======!)'7 2f8,F-/#"%..i..F #)7(FAR9BSc*J;.1G6590:0L9,B-(K1T@@@QQEEGg@@1LLL8Z%@@7?>F9L1G^1gbHR"ZD@!rPXKf2BdV'CC/@K@L@@C@@@@@@af@@@YWDpppp0\, bpbvYN89K)qEs.p:*ae;yp8wN $ /<&w)G(_q/.H18!=dRe> ]*lL5"T=Sx{q8FdaQHWD@zQm Q},%,S8#y'"<,dX&kKpwq'ItOrnz}J?d $\>%|Bp-P &LD@JL 0p+;#+=W  d|`< =;W1{:?r'55={??{Z33!, !1e{h!/'<-2W ZBSQW7GX($3;+.9!*'3fLE063DY1!#d:fy27*B0O%,$J(/76<A_C);>);67'!'FDEYL*)r3#RA(H04%!D<(-I ~+/H(&FQC/3)&6OJK[T,H^Gu9m^/t]$K&5D.h8"8-/;le27' 5@C*n<[zo<'[^+J!&EZt,QVX1KWJL:WASj_@(M+4_4p;l'WhYi!!X GT%/t/?2,j>@u,$ZR%;H(^!Y?#!fxvx*UH?+3J18l%)W9El4(L3s/3C%9C,F]HD3SGJALm,1tXB.uV;'*X207thMU(.=o#dc)@OQF,dP.EQoyW5MePkw BX/?R9MW4O1%RQ9#04/eAVBUxH&Ny@=bb(BUmP?lWedR2^Y;hg@{M]fdA+@.eD;n:9FRNeR*F2DPIv0aTWAQCn!a<qK &$5LGAAATTGTAAGCACAGGCTGTGATTAGTGTATTTACCCACATAAGATTCATACTTATACACCAACGGATACAGACCGATCGTTTTCATCCATCGTATCTCATCTGGGATCCGAGAATCAGGCCCTGGAAGAAGTTTAATTAGATACGAAGGAACCTCCAAATCCTCAAACAGTAAAGCGCCCAGACCTTTAACCTTCATACATTAGCCACATTGAAAGTGAAGATGTTAGCAACAGAACCCTTGTCGAGGAGGCCAAGGGGGTATGCCACATACCTAACATTTGGTTCTTCCCCACCGAGGACGGGCTCGGTGTTGTAACACCGCCTTTCGTAACAATCAAAGCGGATAAGTCCGTGGGCCGAAAGGGCCGTCAAGATACTAGGAAAGGATTAATAACTACCGGAGGCTCTGGCTTCCTCAGGTGGAATCCCGTGTCAGGGAATTTCTTCTATGGGCTGCACGAATTCCGTAAGTTTCCACTAATAAGCAGGAATTTTACCCCCCCATTGATAATCTATGACCACGGCTATAGAAGAACACATTGCCGTACTCCCTGAACACGATAACATAAGCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-23 23:50:44 -04:00AEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt^ASPt^B1Pt]B1Pt]BCTS}BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?TDATA ?ӒDATA ?RDATA ?ђDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~{zLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AM?~gCP1RL?~bP1WD?~kP2AM?~tP2BA??y;P2RL?~p?PBAS??S PBAS??PPCON??`PCON??^GPDMFTrPDMFPPLOC?~YPLOC?~UKPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%PSCAN@SMED SMLtSMPL!!TQSPACA\ySPACPSPACA\ySVER4.0SVER |SVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHPphDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt^ASPt^B1Pt]B1Pt]BCTS}BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?TDATA ?ӒDATA ?RDATA ?ђDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab^FVocMFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~{zLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AM?~gCP1RL?~bP1WD?~kP2AM?~tP2BA??y;P2RL?~p?PBAS??S PBAS??PPCON??`PCON??^GPDMFTrPDMFPPLOC?~YPLOC?~UKPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%PSCAN@SMED SMLtSMPL!!TQSPACA\ySPACPSPACA\ySVER4.0SVER |SVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHPphDYbigphQLcphTRphTR,*^(`P. O~JcW`@0'q qoVU-9k *c@{UaG6EG>dcnxgJB7`DK,*Tijh.iOW/D-]$/pTFV2TVyLyD,kSagv9'i?oX$^io4)X^UM]|ny!.qK|C!^g:G1|*dc0U+O}L6iyNmwP@rB ]:l=&3#Veqy{g_hD6z\[N.6| Vkyc p7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/vO3G`8hC0rOHJJr9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YzXr$cn?:;wWG{uHYs+/e_'UvBVvwp#_T99Wq3F:{SocJy>@ YlGcRb[xy8J9tG>udrV=y`6@TmkcDynWa9?QLwUF3TP.&"D|;f[5kr+E{@2Km}1mf+)FK#5>yz6[]=D#WSW>7.c$8\2[H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NH!V$^1)nQyJT[pc} ?VpSJ<6DR+M%KQ:43:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;N