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(,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRTTTMMMlll&&&000999BABJJJSSR[[Zccbjjiqqqxxw~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8c61a508c26a4873a8bc3ec6d01d99bbSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_22-03_06503130Seq16680134621_134621mfagan96-Well%K1316_B11_Parmentiera_aculeata_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z!<_~,F`z*D^s~ui`bq(C\o~`6rCqM- -Eb C}Hj~aH3% jDtQ)rK*sic^YTPMLKLMNNNPRV[]^ZRH>621257740*%!!%,3;BIQ[i}(g>Hwf2gRyg^[\`htU8sF _ @}p%l A>S !%Ty5K&7tS=0'" #(0:Jc*s VZz3lUHB>:4-'#!  !%&#      !&),./00/.-+(% !%%" +^EBP +?a&   %)*'"     "!   :zm#[C#Kl@  .Z%hMr<    ##"  !#!  $QY$d|f'bg?+$#$#!")0673+!   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'17:94-%  !(/58860)  ""   $%# -<JT[\ZTK?1#                   <AaA&hAY)A"oiKB.bcpKB_3730_POP7_BDTv3.mobtermAGAGGTCCTGGCATTAAGCTGGTGTTAAGATTACAAATTGACTTATTATACTCCTGACTACGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCCGGGGTTCCGCCTGAAGAAGCAGGGGCTGCGGTAGCAGCCGAGTCTTCTACTGGTACATGGACGACTGTTTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGAAGATGCTACCACATCGAGCCCGTTGTCGGGGAGGAAAATCAATATATTGCTTATGTATCTTATCCTTTAGACCTCTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGTTTCAAAGCCCTACGAGCTCTACGTCTGGAGGATCTGCGAATTCCCCCTTCTTATTCAAAAACTTTCCAAGGCCCGCCTCAAGGCATCTTTTTTGAAAGAGATAAGTTGAACAAGTATGGTCGTCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAGAACTACGGTAGAGCGGTTTATGAATGTCTACGCGGTGAACTTGATTTTACTAAAAGAGGTCCTGGCATTAAGCTGGTGTTAAGATTACAAATTGACTTATTATACTCCTGACTACGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCCGGGGTTCCGCCTGAAGAAGCAGGGGCTGCGGTAGCAGCCGAGTCTTCTACTGGTACATGGACGACTGTTTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGAAGATGCTACCACATCGAGCCCGTTGTCGGGGAGGAAAATCAATATATTGCTTATGTATCTTATCCTTTAGACCTCTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGTTTCAAAGCCCTACGAGCTCTACGTCTGGAGGATCTGCGAATTCCCCCTTCTTATTCAAAAACTTTCCAAGGCCCGCCTCAAGGCATCTTTTTTGAAAGAGATAAGTTGAACAAGTATGGTCGTCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAGAACTACGGTAGAGCGGTTTATGAATGTCTACGCGGTGAACTTGATTTTACTAAA+8?HQemz ,:FT`o} #/:HS^iu $.:CP[hv".9DQ\gt'3>JVbp|&1<GS_kw !-:GR_kx(7AKXcp|    ) 3 ? 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F?1P(DFDZ1CVHBS--#08=>G#, <)Q8R)94_W?1F<.c3&,2AJK+_'92=EN966"Z74*V%H d_D6G 7"<L""6FK"AX&WGC  :VHJA>+G3Y+ c'2%/&5 21#GN#EQ Y;,!3BDBV C,a^EQ CLP`C9b6$TO>^T?K=4G=XB:F \b 72'IB'-4#9c09%215$X3W^@HB@N<#/2 ^R%;6:)&+,Z]YOCHB6(A-JcB49+*,(OKXD:9C).T .Q_3;6S T$T]#U#; /AS8A, `#`5 9T+ 'KJ<O!E98 )5LK6L>W*]J!9U)HC<#"7[ >Y/-`9 :/'  Ka5 b'7?*bN=WS.87.F*.;+=YX"IN#E?LB_[:.^R7C]Y1/ A YGY R>E(#@+@44#'@+^F2 V12I^D8EJ KH0V<+ ^6P! ?.ZC @xpn3[UZk<mPZiI8C]Yp\3XZ6Y[JBZ V=MlDnGXG=.8HA'*:GtZ#5)#VWf 7D@L(1wX{y9GkFn &u`*DJ(?npu _x$}FPSW];eMk:]r&"t:5r"d@ Q2qC8)(G,4[][D>V "|m/e <k  KdeHE+.UP!TwN y#xV3 r]@}uP2''n4@52geu,u*pA, +a|" 5/liZfQ}~T^@e:2[ '>Z \O `GY[poR H?VUPye(=xg,ukgiP`T_KCVMSA@L@H77#/%<4))+$,)$%0*& $$         A    .   5  2    &m   --%J  !   C +Z " !, *!L%)*$30# 0 /( 4(+ )++++++4//+H#+*< 9!6J0&372;1'664%+0.e?7D7N[T$C()6X>>>><KDT(=?K-E[2X>>U9PG7QpD4[2m QRG$KJ22ND9:D G2]BGGL2D6s322D>2222>G >C{ppppVp p-Qr9RNp  y\)J((>pNwGWZH&7+@[1.nW:rcCe0b lLKZD@!Id9%` gr(YpqV *7_}0z')?>\#@_P8L[ qd dgdnM\lqd+>j !e*{|Gi43A:s$-|C3+v38dFc8N,T|GY=Nl>SQp,|AU-ULd,<U,@;XnAoj$Ss J 9Q"'g8L,IA>xQ.!>&(/|*GI;7'"%^*B+K.3/,F)('$$*/" %&+2'->7 9. () "r^7PO!m"m4^%TPubq,'=fTg;D! N7.[@d5"6xJ L5&f7~I=8% 3J>'/@Bu8Hu&('sKib+ChDG8FTj#eM0Go;6\EcOBNW-8FfHJ_KR.&-Mr$W$4:<Jnhhv [ t_5q#Om5(`&$>&2;-|Em-`w!Yc.0LZ[DV K&m6fUt#lDNVzMrqK@<LN%]5sN/ Y"y?\Ve?6s_VSDJcs i"ZYto x^`t#,etY_N)EY#Y( f\\t6,W+6)GAGAAGGTGTTATAATTAGGTTGTGATGATAACACGTACCCACATAATATTCGGCCCAATACGGCAACCGATACAGCCCCCTAAATGGCACCCTTCGAGGCAATTCACCAATCCCTGAAGAAGCAGGAGCTGCCATAGCAGCCGGATCCTGTCCGGGCACTTGAACCACAATTTAGACTGACCACAGTCCCAGTCATGATCGTAACGAACGCCACTTCAGGCCCATCGTTGGGGAAGAAAATCAAAATTTTGCTTTTGAAGCCTATCCTTCAGACCTGTAAGAAGAAGGTTATGTAACTCCCGTATGGACTACCTGTGTTGCCAATGAATTTGGTGTCAAGGCCCTACGATCCCTGCGTTTGGGGAGTTGGCGAAATCCCCCTTCTCATTCTAAAATTTACAAGCCATCTGGCAGCCAAGTTCAAGAAGATTTATTTATCAAGAATGGTCGTCCTCTACTGGTTTACTATACTCCATGAAGCCTAATATGGGATAATCCGGCCCCACCTACAGTAGAATCGGACGGATTATGGATCTCTACACGATGGAATAGGATATACGCAAAAGAAAACAAGGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-23 23:52:01 -04:00AEPt?.AEPt?.APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XSGDATA ?XҟDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMAhP1RLAd:P1WDAm>P2AMAvBP2BAAAzP2RLAqPBASAATsPBASAAR2PCONAAaPCONAA_PDMFTwPDMFRPLOCA[6PLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL&&TQSPACA_JSPACRSPACA_JSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB11Tmpr<UserphARphCHR-phDYbigphQLcphTRphTRꁅThis is the confidence range *ABI_Limits..FVocAEPt?.AEPt?.APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XSGDATA ?XҟDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMAhP1RLAd:P1WDAm>P2AMAvBP2BAAAzP2RLAqPBASAATsPBASAAR2PCONAAaPCONAA_PDMFTwPDMFRPLOCA[6PLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL&&TQSPACA_JSPACRSPACA_JSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB11Tmpr<UserphARphCHR-phDYbigphQLcphTRphTR!q*D]{SqmP{QJHeXSbNfP>A0&&e`r/zapM.=!@D:Z>K>D`Vd96M8@/|thLG^r@uPDsvA;DmwC&Y?^dIrLei;(F}K7T]Zj7y!R}*Z8y0Pajeoi@LaiY2]C0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4\KUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^bAmK7^d}|}{B.@k5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQEO=4go X:QJf9/qwAxe<][#m*ZY8|k_R.AQ>UR6? sdWUg>|3)p*]A|kgRHK2][J2%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tu#,xHL?iCB0'~nRnX@EV$2kE}:>&ZgL7:tl|y\@fKl\~:"y:\{\"`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qN^Fu*tw9E^u4[Jn\9-s1tog,s48tNhA&!qH$AEPt?.AEPt?.APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XSGDATA ?XҟDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMAhP1RLAd:P1WDAm>P2AMAvBP2BAAAzP2RLAqPBASAATsPBASAAR2PCONAAaPCONAA_PDMFTwPDMFRPLOCA[6PLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL&&TQSPACA_JSPACRSPACA_JSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB11Tmpr<UserphARphCHR-phDYbigphQLcphTRphTR