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^ ,:0 yW&2(,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRTTTMMMlll&&&000999BABJJJSSR[[Zccbjjiqqqxxw~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0a725f21d3934b66b7150fb528ac54f4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_22-03_06503130Seq16680134621_134621mfagan96-WellK1316_A3_Solanum_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z0Kf~Bf8Pcpx|-L^dc^]bp^2c9E s`SLHHIKMRa{S.W"8EIF=/dH5-,-* pT5lJ* ztpoonjd[TPQW_hnnkd]XVWXYYVSPPT[ep{-b<uJfphO(~?]1 |z{-geREg.P@F%m_ej4 J\]l5dg?{\\hA) !#&*.258<G[|-x I|s8V a?*"*3;CIPU[^_]XPF=5.)&$#"           ?zQ?:hU?R!  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B. a^+Z)0C4*DEAE>(H6a1,# V.7+daYTaLY2H[_G -E K2'ARJSJB=^2c<"_>"51LB<bXJ`/dG&G(6.*-T<-DK;!/LY`A* #'\JKVFGbMY.4]%6"@_AV76Z6VH %H,WZ2cICKGVT/C!N=<9%cX7W1IL .A'5.(_#F<0%)] Y-1#>0PCYX1<J@%#D2]TdMW!/[6:Y@) * "<9/  N)@ [& \&F2OWF2DI /W,a(S(sF,m:9_6L0>3++)}>L4rAF\jY*ZvCKBIc`2mb5Z K9tvKA Le+V}m*^B4_`WJ% \4| Wutv'?zYP i.;^$<38$ ^pB "tGGxpphD9Hy3W}3 `*DOu9&]M9$XeC:s =m &Zl&!*vQljchc\%~kVpYPw$_p/ "~)]r ;nu1z5kd1DQZ}aZF. j b[sa27{71mlMPDWQ2Dnj zbEzO8oCfFBedtMh-e]S %jy $nk(mVemkYWUTbP?@?0OT<F3@H7D%$0%-<0#)&* "             3   +   # ! 1  _ %(%  !0 % <2+ %*/$%1,/3"KP<#U!63A #,6&566 98:E_@4(4(3C-^?=*7>),4<O<8FSKNN66-Y22322)5,,2)BQP>)6,,))ppppNp)<$zppRy8eF8FABh;v,p1*TN*Pj"Mdk7Uf3E",d9d8A5,H LR/d,uOF,mRVg,IJ$% o82W/5D#<pWpO" x87Mf,%,p|!Ue&dDC<aI58XmA,VvF%,.F Ip pZ,dEoda$K8p pa9%S#-'p pLpeNpFI,p  OGnn~9VN,wT"'g,$'Rtun`N%*C!=6zD'@K5C4[2"$# #-*(-+ (-/*6'.0-U3#8IBA')@W.-.#2C6)(!!E,&. +),5A'&#)3 $,1  #"(7 +5"$.')U"!!" 5!#!%*! -)!<D>"&= :8"")!!$2(#%('*#5F"!2'.42E5(I/#0!#&31-'!00L1*F& 5;+2'F-"!2-#")<;233#N/(!('5;$3?)+"(F&-hXR-=x=-d><8";33> UE1J*Q5243!/!*24Y412^6$ #"I$33 45BNv 6B'#5qbD+,G& /$@V707!(9&@'D:?$A,@(2980(2%S+?DUBQXK9/eC14i89<8RjPbT+2W\ }k+1aKAAAAATGGGTTTACTCGAGGTTGTGATGATAACACTTACCCACATAATGTTCGGGAGTATACCCCAACGGAGAGAGACCTATGGTTGCCTTACATCGCACCTCTTCTGGGTGAAGTGCGGCGGAAGGAGGAAGAAGTTTCGCAAGAGCCGACGTATCCTCGGGAACTACGAAGACTGGTGTGCAGAGCGATTTACCCTCCATTCATGAACGACAGGGAATGCGACGCCGTTTCGTCCCTTTGGCTGGAAGAGGAGGCAATCGGGGTAGTGCCTAAGGCGCCCTATTGAGAGTTCAAAAAGGAGGAAGGGCCAATAACGATACGAACTCCTTCGGTGAAAGTTATGGGAGTTAGCTCCGCGTTCCTCGCTGTCTTCGGGAAGAAGATAAAAGGATTTGTTGCTAACATTAGTTTTAAATTCGCCGGTCGTGTTGTTTTGTCTATGGTGAGAGATATTTACTGTTCGAGGCCCCTATTTATCTGGTCAGGACCAACAATGCTTCCCCGCCTCTGCTATTATATGAGGAATAAAGTAGAAGAAAATAAGGAAGTCTTCTCCGATGAAAATAAAATAAATCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-23 23:51:01 -04:00AEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtkASPtkB1PtjB1PtjBCTS|BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?o~TDATA ?o~jDATA ?o~RHDATA ?o~&DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~{%LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP P1AMBfP1RLBbOP1WDBkWP2AMBt_P2BABBxP2RLBoPBASBBRPBASBBP?PCONBB` PCONBB]PDMFTlPDMFP#PLOCBYGPLOCBTPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%PSCAN@SMED SMLtSMPLTQSPACA\KFSPACPSPACA\KFSVER4.0SVER |SVER,,/Scal@Scan@TUBEA3Tmpr<UserphARphCHP:phDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtkASPtkB1PtjB1PtjBCTS|BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?o~TDATA ?o~jDATA ?o~RHDATA ?o~&DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab FVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~{%LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP P1AMBfP1RLBbOP1WDBkWP2AMBt_P2BABBxP2RLBoPBASBBRPBASBBP?PCONBB` PCONBB]PDMFTlPDMFP#PLOCBYGPLOCBTPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%PSCAN@SMED SMLtSMPLTQSPACA\KFSPACPSPACA\KFSVER4.0SVER |SVER,,/Scal@Scan@TUBEA3Tmpr<UserphARphCHP:phDYbigphQLcphTRphTRPy]]~I8[e0MSayQckycji[&`&h2hA:DCDFIt~FRi^aL7(p~XVG ;W/0'o ocDGRFT16Y-e+&5%`7lZ!O)>C9iu2Zv&1x5roz;P]pDuYLDK2NB&_SF`|z&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/YgnCCHY<6(mu y-WLa)&:\.~x|MI>"J'8O[,_a2'Kdb"U"=C!(3by{,ty\bH?6g%3tk?$[WturW>4GPQEdrB-8'i2{ysPl`A?^NHEDX+9m#k:X=<;`O|g+4b|U:h t$+!fIO39ThvTDM,IlE]&l8EE.Om,\eyDWYI!8U-o9D4lxXW[uTs|$@^]?\Hif3qE`HoG83:8\_pf|r,!}&Apu}*qTeP|FMFu}F_7(}Fw+%{y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&HqR7Q@iB\f8!('n Tv`0p?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52rRU)>`V$];9'k0K3AQZ`K'H:{GKipM`{VL7f4AEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtkASPtkAUDTB1PtjB1PtjBCTS|BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?o~TDATA ?o~jDATA ?o~RHDATA ?o~&DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab FVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~{%LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP P1AMBfP1RLBbOP1WDBkWP2AMBt_P2BABBxP2RLBoPBASBBRPBASBBP?PCONBB` PCONBB]PDMFTlPDMFP#PLOCBYGPLOCBTPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%PSCAN@SMED SMLtSMPLTQSPACA\KFSPACPSPACA\KFSVER4.0SVER |SVER,,/Scal@Scan@TUBEA3Tmpr<UserphARphCHP:phDYbigphQLcphTRphTR