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***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRTTTMMMlll&&&000999BABJJJSSR[[Zccbjjiqqqxxw~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8b37ba4ac34a436f8e0a569e236f4dd0Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-23_22-03_06503130Seq16680134621_134621mfagan96-WellK1316_A10_Cupania_glabra_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z 1BR`mz;Q`iq|yeQ;#bD-   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':N_ileUA,    ,GelR9$  :Xuy`J==H\ru_I5&  1MjsY?)  +>OZ_]TI;-!   -@Scnpk]I2  #1AP[`_WJ;-&&.>Pamqk]I3  0BQZ]YOB4&  &-5>EKNJA2" $4DS^cbYJ7$  "3DT^b^TE5%   $4CPVVOB3$   %3@JQVYXTLA4'   0DYkx|wjXG;7;FTajkeXI9," (7FRY[WMA3&   %.5;=<7/&       $4GZlz~p\D.                                                                                                          k@"AiALAFKB.bcpKB_3730_POP7_BDTv3.mobtermTATGCTTGGGATTCAAGCTGGTGTTAAGATTACAAATTAACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCGCAACCAGGGGTTCCCCCCGAGGAAGCAGGGGCTGCAGTAGCTGCCGAATCCTCTACTGGTACATGGACGTCCGTATGGACTGACGGACTTACTAGCCTTGATCGTTACAAAGGGCGGTGCTACCACATTGAGCCTGTTGCTGGGGAAGAAAATCAATATATTTGCTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCCGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGCTTCAAAGCCCTACGAGCTTTACGTCTGGAAGATCTACGAATTCCTCCTGCTTATTCCAAAACTTTCCAAGGCCCACCTCATGGAATCCATGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCTCTATTGGGCTGTACTATTAAACCAAAGTTGGGGTTATCGGCTAAGAACTACGGTAGAGCGGTTTATGAATGTCTCCGCGGTGGACTTGATTTTACTAAATATGCTTGGGATTCAAGCTGGTGTTAAGATTACAAATTAACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCGCAACCAGGGGTTCCCCCCGAGGAAGCAGGGGCTGCAGTAGCTGCCGAATCCTCTACTGGTACATGGACGTCCGTATGGACTGACGGACTTACTAGCCTTGATCGTTACAAAGGGCGGTGCTACCACATTGAGCCTGTTGCTGGGGAAGAAAATCAATATATTTGCTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCCGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGCTTCAAAGCCCTACGAGCTTTACGTCTGGAAGATCTACGAATTCCTCCTGCTTATTCCAAAACTTTCCAAGGCCCACCTCATGGAATCCATGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCTCTATTGGGCTGTACTATTAAACCAAAGTTGGGGTTATCGGCTAAGAACTACGGTAGAGCGGTTTATGAATGTCTCCGCGGTGGACTTGATTTTACTAAA(7LR\lv,8ESbpx!-;FR]iv$1;FS`nw(3>KVbo{ $/:FP]kw $.8DP[ht ,8EQ\gs &5?KYdp|   ( 2 ? 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( :5Z=UD:HD98P S\(7 S1,d cAQ3  T=CH+:4a%@! H;* /&1WV '*9:PH B")I R;;'=]@!@+] -K?WX a$6<G0*[3XQ9R0"RC GWF ZO9%: 4>\PYcP \aHTCN0@O\b`a: =" XA7Z &;Kc>W1:E :1E/:!CY#B5 'A(;A*D@N?U'$4J PV[L@a#XU\3X HGL"<'bUC-+.RGB-5/;N,#>H6,-7bbc!K3< /1 2 3 W%_3*/T IGWJ01 J%D$TY#%4)!D2B J  4 -#Y=CP SJ74&NR& CU@*;D.<.K9F<3,*a53\=77!YS^_Y$0O'=) "(81]",!.bL?3 !V8AF\^ $Q; GP@[7''?T-* S: "IB>2SF) WE8NDH;Q`F b;81S8E&-JVOXJ_*+Ch19nqd:ujJ'm32t7/r KPbB^[MN 1 /Pm] \3nId\1-ty&jPd]xebw7E$P$q@ OdC2n>ys6  TXCId:d}DA}S<vt{?*(J5w(UU6+ E)@eUL0_ElV|TTA4/+A~aO s]rB/ 0} }T2ZoNV=YTe5tC-<"aD,(PkX6,2z]XM+<90bb.h). $(DdT/1E\Cv.nr|hvi+@[(Y(L9]K$X"(d 6fw?q Hgo C83*y\I\amf}lYmbRRPZPI:L\7K8@KQ%7,9C4&8+((6!&$)      %     ,     B$  /7"  YF  &7 -'&&U'^    "! (7(3=)  <&&$&&(C7+  3!I!-9*.*0 5:RL,'S+=IF3%R9(7%8:SE;)f-^E>$C&;#,4!<*1&=A6/E>>[=PO[..SM22.2222>288ppd#p'Bp'h|,!pF{p6P;GZpptF}id,2pKp8BSm,pqvb 0|eF->,4dd6V,<~=Xen8dqp-.%kck~b,E#,y,#dmBDCh`@~,d8poq,d81d<<[d,96OD(7w888`d#p{pS0npL[r'cp'pREHd3BE8 pfp.f#*NE fsJ"p7A3zjhd8}oR)ju@wMxuu*sv/ IX<Y/4`fH?#i|,&eZ%9')#3$(@$16%)-I(J#:,3.&MPj. aI$KcB8,2%;(.\@?=&0 0"$%,1!*6) 4,! 00'+.%(#!<($ G$&45!-+*3-,.O.0# !%&*)#K ">8(3<8@2=PA'!#(*<)%(Z(,-%D$)%#26'6K+!&B#8?#,9#'0?"5$/<+ 2'&!!*=9A)Q3 M07,-$N> 2&-0$2C!A%2?`2(79F7%3#/*#0L==.<&O:+)-2E#K<Q0'M:7-4+*GtX4+.<*/L_P*76Q?/VJI&%45xp<*3iW96Ccd9/|";)<2%N5L-9$,9M6OI0{9@@*4@%JYW36cVYtU##T:0RsB3-77D:5C,/W<W7H0C@H;Q4kZ:eK6S>?B5-\Eer#N4UW]EJPlEZ)f{d &'sATGTTGGTTATAATCGAGGTTGTGATGATAATACTTAATCACATAATATTCATACGAATACCCCAACGGATACGGATATATCGCTTTCATAAATCGCATTTATTTTGGGTTTCATGAGGGTGGCCCTGGAAGAATTTGCGGAAGAGGCGGAGGCTTCGGTTGATGTTGGAACGCGTGCGTGCAGGGTGGAATAAACTCGATTCATTAGCGACAGGGTAAGCAGAGATATTTTCCAGTGGGAGGTGGTCAAGGAGGGCCACGGGATAAGCTAGGCAGCGAATATTAAAGATTTCAAAAAGGAGGAAGGGGCGACTACGATGCGCACTCCTTCAGAAACTAGGGTGGAAGTAGACTCGGATTTACAAGATCAATTTCTCAAGGATATATAAGGAATTGTTGCTAATAATAGGGTTGGATTCGTCGTTGGTATCAATTTATTTATAATGGGAGCTATTTAACGCAGGTATGCCCGAATTCTAGAGCCAGATAGAAGAAGGCTTCGCAGGTATAGACAGTCTAGGAGCAACGCAGGAGAAACGCGTAAGGACGTCTTCTCCGTACTGGATAAAATAATCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-23 23:51:49 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?\TDATA ?\DATA ?\SIDATA ?\ҥDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR P1AM@hP1RL@d<P1WD@m<P2AM@v<P2BA@@zP2RL@qPBAS@@T|PBAS@@R<PCON@@aPCON@@_PDMFTqPDMFR PLOC@[<PLOC@VPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%RSCAN@SMED SMLtSMPL TQSPACA]ØSPACRSPACA]ØSVER4.0SVER ~zSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHR7phDYbigphQLcphTRphTR8This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?\TDATA ?\DATA ?\SIDATA ?\ҥDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR P1AM@hP1RL@d<P1WD@m<P2AM@v<P2BA@@zP2RL@qPBAS@@T|PBAS@@R<PCON@@aPCON@@_PDMFTqPDMFR PLOC@[<PLOC@VPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%RSCAN@SMED SMLtSMPL TQSPACA]ØSPACRSPACA]ØSVER4.0SVER ~zSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHR7phDYbigphQLcphTRphTRV"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49WW!'BA%tuO#Hsf$m=%M:4\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUOT'3S/wDF]6YZ:w{_Gboq6DR+M%KQUfu:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;N `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?\TDATA ?\DATA ?\SIDATA ?\ҥDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR P1AM@hP1RL@d<P1WD@m<P2AM@v<P2BA@@zP2RL@qPBAS@@T|PBAS@@R<PCON@@aPCON@@_PDMFTqPDMFR PLOC@[<PLOC@VPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 7RUNT 2RUNT  RUNT 1 Rate 0RunN""J:S/N%RSCAN@SMED SMLtSMPL TQSPACA]ØSPACRSPACA]ØSVER4.0SVER ~zSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHR7phDYbigphQLcphTRphTR