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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR;TTT;MMjll&&&00099?BAJJJRSRZ[[cbbjjiqqqxxx~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 13d4fb9a146545f089b90a10abbb9880Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-24_03-25_06533130Seq16683134619_134619mfagan96-Well'K1315_F11_Peperomia_urocarpoides_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z'*0:J]ny+9GT\aa^XRMHDA=93+ 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C'6<#(c!3 !##<"  %!    #,!"  !(% #    !("!(* $& ( +$!($! "#*%%;"%) !")#+*'!)*'3$ 3'&)%0&,.%//9" &\73A-Il-hA]2:N$%.E),!/!6B.;%>,*$-8<'*,$/ d@81=!.Jb)d`< <3&H) =7>Q5([;Q2n='*T1/$Y=<:+R+??4YO??fIXIE5LV;Pt*" t9+@ACCTAATAACCATACATACCTCATAGGATATCATTACCTCAGTTGAATTATTACACATGACGAGAGAACACCAACATTCGTGCACCAAACCTATCCAACCACACATAATACGAACCACCCGTCGTTTTGTGAAGTAGGAGGAGGCCGAAGTAATCAGAGGTCCTAGAGGCAGGGAGGGCGGAACGCCGAGGTAACGTCCTCAACTTTGGGGGTAGGAGGGGTGACCAGCGCCGCCGCTTCCTCCGCGGGGAAGTAGGGAATAGGCTCCACAGAGCCTAGACAGTGCCTGTCCTTTACCGGGGGAAGCTGGGAACAAGCGCTTCTTTAAAAAGGGAGAGGGAAAGGTGGGAGTTAAGCCCTTCAGGCTATAGTTCAAATAACAAGAGAATAATAAGGAGATTGATGATTTTTCTAATTGGAAAGAAGCATTTGTTGGTTTTGAAACAATCAGTAAGCCTACGATATAAGTATATCCGTTGCGTCATTGTCAAGAATATTATGAGATTAAGTATTATCAATCACAACATCAGTGTATTCCATATGAGCAATACTCTGTGGATTAATGACATTACTGCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 05:14:29 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS}}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM@gP1RL@c4P1WD@l4P2AM@u4P2BA@@yP2RL@pPBAS@@StPBAS@@Q4PCON@@`PCON@@^PDMFTPDMFQPLOC@Z4PLOC@UPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL((TySPACAaSPACQSPACAaSVER4.0SVER }rSVER,,/Scal@Scan@TUBEF11Tmpr<UserphARphCHQ/phDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS}}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM@gP1RL@c4P1WD@l4P2AM@u4P2BA@@yP2RL@pPBAS@@StPBAS@@Q4PCON@@`PCON@@^PDMFTPDMFQPLOC@Z4PLOC@UPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL((TySPACAaSPACQSPACAaSVER4.0SVER }rSVER,,/Scal@Scan@TUBEF11Tmpr<UserphARphCHQ/phDYbigphQLcphTRphTRMr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;N }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7Z(VIl9?D^mJjEJT]}I2\2s7yh&cDFH#EgPX.:=2AGHF@LeP *">BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|Bq_NU4UbKjZ*~Uc._i+9Vu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnPk=o?eUKGM5J_bi(l{~Gj`X0?888%g^^7FDg;AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTS}}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM@gP1RL@c4P1WD@l4P2AM@u4P2BA@@yP2RL@pPBAS@@StPBAS@@Q4PCON@@`PCON@@^PDMFTPDMFQPLOC@Z4PLOC@UPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL((TySPACAaSPACQSPACAaSVER4.0SVER }rSVER,,/Scal@Scan@TUBEF11Tmpr<UserphARphCHQ/phDYbigphQLcphTRphTR