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    & Yd,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR;TTT;MMjll&&&00099?BAJJJRSRZ[[cbbjjiqqqxxx~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: cb1b1a56419d450db8186cb5057f1600Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-24_03-25_06533130Seq16683134619_134619mfagan96-Well'K1315_E7_Caesalpinia_pulcherrima_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z59@K[m~  &/:EMQNG>61147971) ~wpfXD.~}~~~}zvsqpqsuuusrqsw}4X-mA%gS` k#f/}k\PGB@AAA>941//01222111110.,+**++,,-./00/-*('(*,/0112247:>ACFINWcu7pC@}:Rdr_RJEBAACFJMOONLJGFFGHHHFDA?=<;:741../379:73/*'%$#"  %3Gc'e]%eG2(*9T}P.1Rod40<MC5{&pQ@:;@DGIIIJMOPOLF>71,)&%%%'),//.,)((),/00.+(%"   !!! 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)8<Ia"AC\_ ! >\OK.152[HGL5 UH 93&`b1QZNMM0RB\:M^O`'I4N=>?4&.0<%I<HJ0QB@J1' C13O#O^2+A 2 G=+O.[9-W:\(<8?c")M(@UG .C ?; 9 !Q3W,=^RD9W&" / ,)]/U^ =B@*S(Pb;% +1-97P]b2E(2<7XD3H.P ;2{zVU51Tb^AN#YlyP  -]x4A-=U{ 7dH9l&>R9w5op7@}UOR C;Y=8I< Z, J$"KN;D~NfKy[PK[gC[R.ZB {Jmj<mN lI2PW J%O 2oAw"NEh)5 UKS)+_atu +fDsbOw+h k[se5I=-&@uGkp-Wsi{[cv"bcerR V ,3rJ!6{3@#mKZ;+7+vv4vz\#!7 N \G xJSi>|m;^H\5~aHT7Ktj+mLW[, !2QAo|k=hfA>* b P|Bvi%S& `0,okefcofff_@LV\?OKD;84;IM)@(7//971,.50. 2/!,      '$  $        *                           !"$   %" ' ##+*!$(  "'!$/1!.'&/ ,1#)4'#'0)+*,00'1?7/11>2.4><35(15C468!:9J::9=A6S:=M:@:Z2=CM9+;TTAAQJ@CAqQEH^HZGSCMV?\\NL_XA?QMMKRa^RZkk OZZ`NYP]MSrda`[_PUR`Zsd^}ac[^QhKKKKTTTTKZztsP\'ppppppppV5D,5eeEp3adk(op;d rIppD 858^s[>W7d8+Qu5(p,nWd+emL\R dGUPA=;Rx,pZ B"4UpMPp_('ViM8Q*l0M=Zp$VW[j$V[,!g,<p]^88M8}pHU;9 5Spfz!\5}@1# u'5:TA3lau!0  !$!.D(/  !L  >1#' f,P~)#1 (  3) <#'###)/(#) %*$22 # - ( &! 3%  +5 )8*,'" # '4!%OL(,%% "7'*"=906$ %.)'"%,""2-)%!3-,$)=-&'8C#)( ##",6M1("#7931"01),&-&!/2N1,&%3(';#<' /A69.&;,+/F# '#:!1OCCl(Oc@.(1I+1$ #PS.9)#3.J1/#h-?%9#(A3*%;.&9?LSHH4D.PX:2i5-69 %L+1F<J+12N:W?NM(90,BZ7Ev>E='0(M''YPTO7P/ :yoTKd$'CCCGTAATTAGTTCTACACATACTCATAGCATATCAGGACCAAAGTTGGATTATTACACCTGACCAGCAAACACCGGCACAAATGCACCAAATATGGCTAACCACCCATGAGGCGAGACGCAGAGCGTTGGGAGACCTTAGATGAGCCCGAATGAATCTCCGATTCTACCGAAAGAGAGCGCGGAACGTCGTGTATACCTATACAATTTTTTCCAAAGGAGGGCACGTGCGAGTTAGCGCATCCTCCTCGGGGAAGGCGGAAATAGGAGCCTCCCGAGCCAGACACTGGAACTCCTCTTCCACCACACGGTCGAAAAAAGAGCATCCTTCCCTGGGAAGAAGGAGAAAAGGGATTTCAGCCCTTCACAGAAGAGTTCGAGGTAGAATAGGAGAAAGAGGATGTTGCGCCACATCCTGCTCCTGAAGCAGCAATTCTTGGGTTTTTAATTATAAGTACTCCTACGAGATATGAGTATCCGTTGACTGGGTGTGCAGAATATTAGGAGATTAAATATTATGCATTATAACATCAGTGCATTCCACAAGTTCAAGAGTCTGTGGGGTATGACAGTATGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 05:13:59 -04:00AEPt<AEPt<APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtbASPtbB1PtaB1PtaBCTSzsBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?!~BTDATA ?!~BDATA ?!~BQEDATA ?!~BχDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~xLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMP1AMBdP1RLB`P1WDBiP2AMBr$P2BABBvP2RLBmPBASBBPFPBASBBNPCONBB]PCONBB[PDMFTPDMFMPLOCBW PLOCBRPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%MSCAN@SMED SMLtSMPL((TySPACAYw$SPACMSPACAYw$SVER4.0SVER zhSVER,,/Scal@Scan@TUBEE7Tmpr<UserphARphCHMphDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt<AEPt<APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtbASPtbB1PtaB1PtaBCTSzsBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?!~BTDATA ?!~BDATA ?!~BQEDATA ?!~BχDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~xLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMP1AMBdP1RLB`P1WDBiP2AMBr$P2BABBvP2RLBmPBASBBPFPBASBBNPCONBB]PCONBB[PDMFTPDMFMPLOCBW PLOCBRPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%MSCAN@SMED SMLtSMPL((TySPACAYw$SPACMSPACAYw$SVER4.0SVER zhSVER,,/Scal@Scan@TUBEE7Tmpr<UserphARphCHMphDYbigphQLcphTRphTR8Bb:({.#X|L=6XO?Tve!@.%J^K'p=mjzs,t5/`3T6YJ:0/.p9;4^;5s#oC&Opau_l_e5 }37Rgx|ff[`#|D5x_7&OMb'~6/lD-b)jJJTMF4Fi%P p'Icf]4'Uw\Pcc{\A_}$A>AR5Z(yHPx%bg*p/YgnP:Y'e(nVcg'2k)1"X\FqUh]gt/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuU$HTl:h~6O$@v=aiBx[FohiXN"}a8rOrm>vdAVE+w4,!a>b S"e`=F%Sam$[W,sK`FYYi]yFy'kK-vUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>Ube\W'KFkaqQltX[3>>B~:^gWiD!}%`;pS8mu7y\f9!:Ey}V(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i,ZikPvypAX~L=?-XPKw+[_g4#q&N7u`GcO581W+V|oOr7w>PpZw!Q)9wkf/6W6x| /GU-u}F_7(gFk+J{y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`Vcy[*oCf$`6A&03d02R P,hzp!tY`