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"    Yd,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR;TTT;MMjll&&&00099?BAJJJRSRZ[[cbbjjiqqqxxx~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d932dcacc518402c8a2712e915a81b70Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-24_03-25_06533130Seq16683134619_134619mfagan96-WellK1315_A6_Cnidosculos_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z"(.22100258861*# }wrmjgec`^]\]`bdeda]ZWVWZ^chloolieegkorsqnlmqw~Ak I)|#s2Zr|xfGM%cD taTJ@70)%#"!!!"#%'())((''()+-..---/1342/*&$$'*-//.,**+.27<?BCDGLVbqZ=W_hq+p\OGA<61//1467642112455543220.,)&%###"    &6Jc?zQlRA88ASr6#>2`qw# {U|V>0)'(,3:@DEB?:63/+('+8PxAD[2X4        4d2k-MALj4Fx;i1       .YIAaY+zb,          ! #%'()*,,,*'&+:WvKU43G;9f*X{2l;WpiN hB.3:N{g*)^B(%Rn1 "$&(*-.-(        !/_n% n.            !"   > ]I,5bpDtB%.6<>=82,'B{; #~!  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EKy"-+!''1 !0!++$ "'&P )!*(" #)%&.1%4A1*8/E-112!.7<;$#&:( ( &40%#+=''0-  &  >J?!-%6#H+6(/J,-4&&F)$*%6#<!:oE$)2AE91239<A"B' `$E)+:*'.6&+3, .6$'2A.*;*$?C#+@'%/@#&B!6BBL$'s\B0&,55<1'(*o"<""49~-MS$C+/'*47>/3'#F_6G?2B GO&s>:#1s!L*J40 C-#R13^77A-6)D!;+g3H 0*<)8$!3DG7/ICiSf9= lYdk\SDP59AAAAAATTAGTGCTACACATACTCATAGAATAGAGGTACTAAAGTTGAATTATTACACCATCCCAGCAACCGCCAACCCTCGCGCACCAAATCTCTCTCACCACAAATACGACGAGCCCCCGTTGGTAGGGTAAAATTGGAGGAGGAAGAAATACTCTCCTATTCTACAGGAAGAAAGCGCGGAAATCGCCCTAAACGTATTTACCTTACACGGGAGGAACGTTTGTGGGAGCCACACCTTCCTCCTCGGGGAAGGGGGGTAAGGGCGCCTCCCCAGCTATATGGTTAGCCTCCTCTTCAAGGGCAAGCTCGAAGTAAGAGCATCCTTCAATATGAAGAAGGAGAAAAGGGAGTTCATCCATACACTCAACAATTTGAACAGGAACAGGAGAAAGATGAGGCAGCTGATGTTGCTCCTTCTGAAGAAGCACTTCTTGATTTTTTAACTATAAGTACGCAGACGAGATAACAATATCCGCTGGGTGAGGGGGAAGAATATTACCACTCTAACTATTATAAATCACATCAGTGGAGTATTCGACACGATCAAGAGTCTGAGGGGTTATAAGATAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 05:13:52 -04:00AEPt<AEPt<APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtkASPtkB1PtjB1PtjBCTSw$BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA >}zTDATA >}z2DATA >}zODATA >}z&DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE0LAST~~uLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISJP1AM@a[P1RL@\P1WD@eP2AM@nP2BA@@s[P2RL@j[PBAS@@MPBAS@@JPCON@@ZPCON@@X[PDMFTPDMFJPLOC@SPLOC@O[PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%JSCAN@SMED SMLtSMPLTySPACAZjSPACJSPACAZjSVER4.0SVER wSVER,,/Scal@Scan@TUBEA6Tmpr<UserphARphCHJphDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt<AEPt<APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtkASPtkB1PtjB1PtjBCTSw$BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA >}zTDATA >}z2DATA >}zODATA >}z&DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocnFWO_GATCFeat//?GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE0LAST~~uLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISJP1AM@a[P1RL@\P1WD@eP2AM@nP2BA@@s[P2RL@j[PBAS@@MPBAS@@JPCON@@ZPCON@@X[PDMFTPDMFJPLOC@SPLOC@O[PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%JSCAN@SMED SMLtSMPLTySPACAZjSPACJSPACAZjSVER4.0SVER wSVER,,/Scal@Scan@TUBEA6Tmpr<UserphARphCHJphDYbigphQLcphTRphTR4{,|Qv%_F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBI.8#5+yvUR> TVovml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MrrG]ZQU7B)JT_\T+;8p($AP(m"`Tt|9R|H@ Ki6t{_"#kt72We<>/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#VihGxNM4p:K2O1MK*Q)HAL/FTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gW>i;{nI YI=pI}JIXEG%w8.2_/ KRZHlEDx8_1tm9i2=SM7.c$<\U[!iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k0WC.%oXB{H3<(}{Uw2yy>T3pb}0Zz>zWvW9/AEPt<AEPt<APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtkASPtkAUDTB1PtjB1PtjBCTSw$BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA >}zTDATA >}z2DATA >}zODATA >}z&DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocnFWO_GATCFeat//?GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE0LAST~~uLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISJP1AM@a[P1RL@\P1WD@eP2AM@nP2BA@@s[P2RL@j[PBAS@@MPBAS@@JPCON@@ZPCON@@X[PDMFTPDMFJPLOC@SPLOC@O[PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%JSCAN@SMED SMLtSMPLTySPACAZjSPACJSPACAZjSVER4.0SVER wSVER,,/Scal@Scan@TUBEA6Tmpr<UserphARphCHJphDYbigphQLcphTRphTR