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' Yd,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR;TTT;MMjll&&&00099?BAJJJRSRZ[[cbbjjiqqqxxx~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6a386f3aa2bc421a80655ee132f9bba4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-24_03-25_06533130Seq16683134619_134619mfagan96-Well!K1315_A10_Sabicea_mexicana_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z4579::73.'   |ywvvvxzzzzyxyz||{yvuvx| .?Qd}"8IT\ciljaP7hK, {rlf`ZSLFB??@AABAABCEGHHGEDDFILORSTUVX[^`bb`]YVSRQQQQPPPPOMLKJKNQUY\_chp}0b'r5^zlF1K scZVTTTUW\dq <[zsV6oXF90+(&$"   ,;NgTSmTC;766420/18DTi6ml7q_SJD@><<=?ACCA=71+(')+,,*)''()***)*+-/1223344441-)%"!"%'('$   !! !!#%&&&%%%%&&%"    '7Np+| 5A/y|tG+ZNu>   !! 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(tEX>3ckPw1ea5er>4 |&$CKitojF9Cv0ZoLewhF|{=  FMCIm+<)2 qD. D%\T*rn'{j.Xw8Y%Khf=CBO_~0KWA?}1/2sxZ<_H-4X Rfv }u6)S<4nvbd:t87? h pP&RXq5x7#eIzqqci]qdk]5N[F>ZLF7267;><A*'/=;+52.-&,% )B/              &                          !   $$ #"#"#!"!87-$.%!&#0&#&"3**5 "#/(2)!1%':%6+43251  4&3335H@3N5)3#;8O9;A58E16EIaHEL:[@N9w89NHI/:-?:AMWwDF:G[HWVPhGIFWV@TJMI\`oECJ\D\VZ_UKRdXY_gVSYWqY`vVvbcfeUf\lmqhg`^lfgSeMMMMEEEcE_&M p|ppppjww:ndIep-p%#17`<do-., $8j,x J4&WX ,G_.Mdzpppp'o,K[r!#Qd^VJApe;dd.T~@)RRMg},do'7p>.5Ntprppp+HV>`p;C.p  8i 4y. #4yS0(O79G5+i%R'/P,.tA0[`fBR e~'"0 LX )iu94 + * ( X!'  23 Gn)###*2$-%+%1(*%$?>  71 " 1$ !!#'!$ (.):%2%& "*)5 +#(' * %0&/!+9(/,/3;, &'##!##$*+3%F3%!(,#'&$ *G!(&!)4+ /1*!7'9,$4S!6"(80.0!5%/&79=*(JA7=&++*>$3QEG3QT/:?8Z(8#+,.N2<)&4W%Yp5>-2!*&=?/,#3$u =3?$VOd/@Q$E  'DV8>>$:1F!e# A[!jY:Ia+0 !F7%9gLF#79S*&!%-[ZV=MWC(_.=0;j.;qO>8 AAAATTAAATCTATACAGAAACAATAACATATGCATATTAAATTTGAATTATTACACCAGACGACCAAACACCAACGCTCATGCACCGAAGGTAGGTAACCACCAATAAGACGAGTCAACCGTGGTAGGTAAAAATTTCATGCGGTGGAAATAATCTTCTATCCTTTAGTCAGAGCGCGCGGAAAGCGGAGTTTTCGGCCTGAATTTCTACGGTTGGTGGGGTTGAGCGAGTTACCGCTTCCTCCTCGGGGAAATAGGAAAAAGGCGCCACACCCACTAGGCAGTGATCCTCCTCTACCAGCGCACGCTCGAAACCAGATCATCTTTCCCAATTTCGAAGGAAGCTTGGGATTTCATCCCTTCAGGCTGAAGTTCGAACTACAGGAGGATAATAAGGATAGTGCGCATTTTCCTACTAAGGAAGAAGTAATTTTTAATTTTGTGACAAGAAGTAATCATACGATATATCAGTATCCGTTGCCTTGTAATCAAGAATATTAGGAGTCTAAGTGTAATCAATTACTACATCAGTGTATTCCACACGCGCAATACTCTGTGGAGTATGACAGAACGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 05:14:25 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSzBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?6~lTDATA ?6~l'DATA ?6~lQDATA ?6~lDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~y@LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNsP1AM>|eP1RL>|`P1WD>|iP2AM>|rP2BA>>wP2RL>|n PBAS>>PPBAS>>NPCON>>^XPCON>>\PDMFTPDMFNPLOC>|WPLOC>|S"PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%NkSCAN@SMED SMLtSMPL""TySPACA]rSPACNSPACA]rSVER4.0SVER zSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHNphDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSzBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?6~lTDATA ?6~l'DATA ?6~lQDATA ?6~lDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab$FVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~y@LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNsP1AM>|eP1RL>|`P1WD>|iP2AM>|rP2BA>>wP2RL>|n PBAS>>PPBAS>>NPCON>>^XPCON>>\PDMFTPDMFNPLOC>|WPLOC>|S"PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%NkSCAN@SMED SMLtSMPL""TySPACA]rSPACNSPACA]rSVER4.0SVER zSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHNphDYbigphQLcphTRphTR2I\(f^t8 e,&o.]/+M;`8>>~/H'JKo4<"}3C;PGYTe3"Q\|F{?5[3:im;S!?a9TghW~^N@.]neZ_kP)X^UM]aD&Wr`s;bqz^]/\4'\%E:"*lu`>U{mwYgE{}Vm)HNtXehkT/n@Z- O{C8T`T*#sKx'SF*$6elkgah(mae$*6I|t+7@7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\boF3uXB23yKnu3B7UVxXm}j3q6A@ wvr]{;:Y2WHbxUM~*-6)H_v&'Y?sxWlwMXG9{k"K-Dty{J$XXt;J0Fk(1=)Ak&Ln96] G$*fd6>Ua[!p?p5"?uL2'z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS:Q&:C!g61CfFc':Z^mDt4)Mz9n#mUmIppe Rm8}'+-v?U5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,|`Dp>H,o}Qm4'p=R .*}Lq{F}u^zq+|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDT7B1PtB1PtBCTSzBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?6~lTDATA ?6~l'DATA ?6~lQDATA ?6~lDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab$FVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~y@LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNsP1AM>|eP1RL>|`P1WD>|iP2AM>|rP2BA>>wP2RL>|n PBAS>>PPBAS>>NPCON>>^XPCON>>\PDMFTPDMFNPLOC>|WPLOC>|S"PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT :RUNT  2RUNT 0&RUNT  :Rate 0?RunN""JZS/N%NkSCAN@SMED SMLtSMPL""TySPACA]rSPACNSPACA]rSVER4.0SVER zSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHNphDYbigphQLcphTRphTR