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***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=UTTJMMlll&&*00899ABBJJJSSS[ZZbbbjjiqqqxxx~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 590ad77fc9ef49629e0f9b36148aef3eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-24_01-38_06523130Seq16682134617_134617mfagan96-WellK1315_H10_Commelina_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zflz1CQc~5Jb~  +6AINQTX^elonib\Z^huxog]P?)|U0 _:=d+\ wY?+ xgR8p]J6  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[LG7(<[7/] U01!<PW=_7&L5[ FO1+O6&MOIL# M(6(XZAT'"MXV\Y02-&>#cM9^O \56_7&$BO:>'G>+HBUY!OXHb1CGOHCN'- G[6\Nb'W1: 8;Y$]21?Z2>/-SG T0>T=&6 :BE^=W<F-Z 8Y7-Q;*@!,X(8C :& 7`ND< WCGA'Z4\8FCccO") `I 5 .IIZYa!E7R3]U"K.-OK THR (NJ/.S?X /-? 16;:P D,bPX6U#_=C'9A,5C&%DR,;?Z1J% a?cI\ aARQBCOH8$ODM>%M;ALO; P65`b`KI2M)[K+YT_>C1J2MD7G!#U/M+* 6MR;LY_C2!IW K: W74D5[?b 3mFOy?)YVt"|e 6:L'Ef!: >G?p> z[z2;>K *L7,+L)P6N^-@A8s8,<3B-yohbyR  JR-#[_L-G`=nz.Rq2oxJbr"}BSsBuS:>3o?60pRlkbj1Q;)2t-J T>VV9,}*,>d4$[d+2d@?#M#!quc$#u%&_4&Y^z:795`?0JBK2w M5g&h%4+c|.t31G*@Pg @</@x,(8l;" pV63 10.t}P#Rd=l{  (x ABzsdzehu[fb[sTxaeMgRAFST@NHJJC =9?K4**20 60$&+'9*6-4)##!)/$#'1%(<#$'#'&,,$9*.#%# #!3      !      "($3%4('""#,-%#'@ -'&$;2"")(C)- 93O:4$95 :'359'*(1.*6#,9(BB;&(?'/EF2>84C7<4.A7*34CD>AP<J/58NX;OK@RH@Mt8CA<>BSRN;?9>ISQH;M\^[KNCLQINYEUP]d~eUS\\\\fG`nvic^i\[J`\Zmhmcxmjrnk\`Yrkbo|d\d_aplshg|jbzy,ol~fp}d{ iiuuuu ~iiuuuZ}xhvvv|[WQbvvuvuvuvuippE=KpJpnw9>%CdZ,d]y.8d#v8dMT,, =Xvd Sp=  ?p8,7H7F/8vB\ u+j/?pxS^hVy$0#p'[._ppYoIp0pyp8pC [t5`&!z|j0)zM ;5#%Ahfkqi?++5.xA! Z&;fx./3TI'$+@R2?1+(=T 9+M7j'%#18%.1%7  F#"!('*#-)),8"$"&!1-#_i12 #7A:!,''&.*!0$'B';3(@4+3 #*/,B/8%N-"$" M%- $MCI:3194L%6'#-%#5)E18N0 ),D9+7NG"%'?54"$&2$+9%P16A:)F"-7H71)8018'1E^)30/J-2:3 a)1K>79-#*'1a47].#$&PF@(;1jH/!3.X/s:Hog;3,R<C <`/UE6I+F_S-M<9r9W6&3F:\h}SA!8G#g`18kN"=d4G~cctfhAM;6bJ}t;iX1z^28cM-U=qg[lY@]tHukS^nmd-fi]zB\G9mj?|*{o SGAGAGTGTTATATCAACTGTGATGATAATATGTACACACATAATACACTTGAGAACACACCAACGAATACAGATATATAGTCACCTTAAAGAGTGTCACTTCAGGGAGCTGAGCTTCGTAAGAAGGAGGATTCTGCGTTAGCACCTTATTCTCCTCCAGTTACATGAACATTTGTGTGCACAGAGGTTATAACAAGTCTAGAGAGAAACACGGAACATTATTCTTAAATCGCAATTTCGACAGAAGAGAAGACTAAGGAAATAGCTAAGGAAGCGAATATTCAAGCTTTAAAAAAGGAGGATGGTGCAACAAGGATACGCACTCCTACGGAGGAAATTATATAGGTAACCACGGCTTCCCGCGCTCTACTCAGAAAGAATAAGAGAGTACTTTCACCTAATACAACGCCGGCCCTACCCGTTCGTCTCATTGTCTCTTTGGAGCGAGATATGTGAAGCACGAATACTCTCCTTTTATAGTAATATGCAATAATGCAACGGTAGTTTTAATATGCGCGGAGCAATGTTGGAGAGGAGAATAAGTATGTGCGCCTTGAACCATTATATATACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-24 03:26:30 -04:00AEPt?cAEPt?cAPFN T|APXV2APrN 0APrV1APrX}}0ARTNASPFASPt|ASPt|B1Pt{B1Pt{BCTS~BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?ZTDATA ?ZDATA ?ZS9DATA ?ZғDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~|LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM;vhvP1RL;vdP1WD;vlP2AM;vuP2BA;;zNP2RL;vqbPBAS;;TcPBAS;;R(PCON;;aPCON;;_PDMFTePDMFR PLOC;v[PLOC;vVPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT &-RUNT  RUNT RUNT Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPLTGSPACA_SPACRSPACA_SVER4.0SVER ~SVER,,/Scal@Scan@TUBEH10Tmpr<UserphARphCHR#phDYbigphQLcphTRphTR,This is the confidence range *ABI_Limits..FVocAEPt?cAEPt?cAPFN T|APXV2APrN 0APrV1APrX}}0ARTNASPFASPt|ASPt|B1Pt{B1Pt{BCTS~BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?ZTDATA ?ZDATA ?ZS9DATA ?ZғDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabmFVoc\FWO_GATCFeat//-GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~|LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM;vhvP1RL;vdP1WD;vlP2AM;vuP2BA;;zNP2RL;vqbPBAS;;TcPBAS;;R(PCON;;aPCON;;_PDMFTePDMFR PLOC;v[PLOC;vVPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT &-RUNT  RUNT RUNT Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPLTGSPACA_SPACRSPACA_SVER4.0SVER ~SVER,,/Scal@Scan@TUBEH10Tmpr<UserphARphCHR#phDYbigphQLcphTRphTRTFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh("Z1'!r$^oni6O3Iw+ *.![6YgCoH6iAC?-4$&)-%TwK[*oCfty5lYMI){mnjH[$.}RZ#3|>oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'G,lwdRadJsV&=o?eU&GUkJZm{#(c&Hhb+]A:LzZU+$,U8>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt?cAEPt?cAPFN T|APXV2APrN 0APrV1APrX}}0ARTNASPFASPt|ASPt|AUDTB1Pt{B1Pt{BCTS~BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?ZTDATA ?ZDATA ?ZS9DATA ?ZғDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabmFVoc\FWO_GATCFeat//-GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~|LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM;vhvP1RL;vdP1WD;vlP2AM;vuP2BA;;zNP2RL;vqbPBAS;;TcPBAS;;R(PCON;;aPCON;;_PDMFTePDMFR PLOC;v[PLOC;vVPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT &-RUNT  RUNT RUNT Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPLTGSPACA_SPACRSPACA_SVER4.0SVER ~SVER,,/Scal@Scan@TUBEH10Tmpr<UserphARphCHR#phDYbigphQLcphTRphTR