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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8SSSKLfjj###---669??FGHOPPXXX```gggooovvv}}}<<<<<<<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 393029da2b6b458eb6189b5bdc524370Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-18_21-49_04183130Seq16413134048_134048mfagan96-Well#####"#######"#"##############""#"##""##########"#######"#############"###"######""##########"######################"###"##"#############""#####"##########"############"################""####""#"##############"#############"#############""#######"####################"######################"######"##"##############################"##########"########################################"#############"######"###"##"################"#"#"###############"#"#########################"######"######################################""#############################"##"############"#############"######"#############"########""####################################"####""###########"####################"################"###############################"###################"#########"###"#########"#####################"####################################"############"##################################"######"###############"##"#######################"#####################"#"#####"############"##################################"############"################"######"###########"####"####"#"##################################""##############"#####################################"#"#################################"###########"#"##########"#########"########"### 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(  RMF:\7-0/> GO>.\X>U]$%"#U[9,CCB=I]).#(2*#":_\AdMC<5JR$'8,c A7 5X?*FA8P^1:FL">L>Z!)=E&[41 -)CG04:3EDX bL&Y 82>ZVS: 1(JO;dLR7\U,[![QR7"W> Q>b8+2(KGSRJ2T N[&O\K+DX P I]8=Q-bNHLCJH 8!E9G9F/]_[G% V=0(K c1P?F7S!Y+P]4`$>HY^D^* 6)QL"b6>EDKVKSI=:TAG%;2M[E#3']18 "58&>-/KYFA>M:N@"#B D*0=aG#B2XP/@3D2Z^I8I%6'8+'UYEZVBCM N:GKXH(7(+""@%Xa@<P!.NX U"]KC 2 4[Y ]4VNI#F3B G 7H- Q6F,<V8'FU3X?@ SA<0K<OZ@5S%UTS _Q[N#`]<0R<#c>NI0b2PW FBP%BD<-;ECLDb2b2L $]Q^lJ >)A*qn)UDi0v; pbU 4.CSI$D{C/M)8xz>%!v" #>/:1  -E}[}Ajy{%+\Dl d2xe\W_eH|U{/q v+g]]qZaB": tv XOZf3T7)rUnBh.=s#UmBSLcGafLT'wI=h_H"2qV$h4~frHHs7~~W! 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(#$15 $&(&! 2> U+&! $1(%!J$ $+&E!0('4*.%,ST Z9% &  2!)-(*+>&411G5'@*F-@_ y,#!<Y0!8'6*5%80!**{' *% BF*M+@lXZh1=9D6(4Y4@9.;fDX)Z*6(H9nK=7>9"r_DJk\v-beZ[6_oF /D\)ebjPl`Fp{P?`wM*1{>EwLtR X /]LQh%E@R>6mUlhkdg c?yguWcSs'mH'X e-p~uq 5g;v @InFhc*t2qAGAAAAGTGTGTATCGAGGTTGTGATGAGAACACGTAGACACATAATATACTGGATAATAAGGCACCGGATACAGTTATATCGTTAGATCAATGAGAAACACTTCACGAGGAAATGCTACATAAGGAGGAGGACCCGGCGCTAGAGCCTTCGTATCCTCTAGAAGCACGAACACGTGTGTGCACAGCGATACACATTCCCTTCGTGATCGACACGGAAAGAGATTCTAACAGATCGATTTCGGTTGAAGAGGAGGCTAACGATATAGTTGATGAGTAGCATATTCAATACATCAAAAAGGAGGATGCTCCGACAATGATAAGAACACCTACAGTGTAAGTGATGTAAGTATGCTCCGAGTTCCGCGCGCTCTTCCTGTAGGATATCCGCGTATTTCTTCCGCATATGATGCCACCCTACGACCATCGTCTCATTATATCAATGGTGGGAGATATTTAGTGCACGAATATTGTCCTTTTGTGGTAATATGCAATAATCCATCTCTAGTTATGATATCCGCTCCCCACTACTGTAGAGCAGAATAAGTATGTCTTCTCTGTAGACGTTAAAATAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-18 23:36:55 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtgASPtgB1PtfB1PtfBCTS}BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?@TDATA ?@DATA ?@SDATA ?@HDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~|pLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM?~h9P1RL?~cP1WD?~lP2AM?~uP2BA??z1P2RL?~q5PBAS??TPBAS??QPCON??a|PCON??_=PDMFThPDMFQPLOC?~ZPLOC?~VAPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 1RUNT #RUNT  RUNT "Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL!!TGSPACAewSPACQSPACAewSVER4.0SVER }SVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHQphDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtgASPtgB1PtfB1PtfBCTS}BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?@TDATA ?@DATA ?@SDATA ?@HDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabTFVocCFWO_GATCFeat//GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~|pLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM?~h9P1RL?~cP1WD?~lP2AM?~uP2BA??z1P2RL?~q5PBAS??TPBAS??QPCON??a|PCON??_=PDMFThPDMFQPLOC?~ZPLOC?~VAPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 1RUNT #RUNT  RUNT "Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL!!TGSPACAewSPACQSPACAewSVER4.0SVER }SVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHQphDYbigphQLcphTRphTRs)B23Hj.L3`|z&mm,_?DB~P^lQbaeX7SoR:+:j,$}<7-? |EXoW3Y~+ctPp%!++(aQQ*G]mysG(B-O*@NzRfu4WyYZ(Wa:nuXBT]qK_^_<~|Rs5AVih5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9rgzE<=#d1a-c_[O~}4}ceGf+Ko[!AETRF[+]18`F7IOoUVn"eHts'MP(D`~<)l47y!R}*D]d0oA{Qk@ /kXSE@a!)DS *n&-HOnO@5}"a'3.5s\T7}{W_z>FfM~V0Nt9c'gUR(}u2EwDrtR7R>(hZ$~I!|spN$t.D~F45$\j|KA[Lz2fJq3IxdO#rTiEBQ~HFZGkS@R[$K~z[Zh6Ot:/`C?QjigQ}QH8EE.Om,K]yDWYhY8|kN_uw >xPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_MghW~^(';_/C]2ZE<}g6M>P:Y'e(nJcqM2t012#\FPUW,Ut/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuUIcTA?i]8VRJxG57'9\47.<<_