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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8SSSKLfjj###---669??FGHOPPXXX```gggooovvv}}}<<<<<<<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ab6b42db5e194f95a5ea542c54b1a4a7Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-18_21-49_04183130Seq16413134048_134048mfagan96-Well#####"#######"#"##############""#"##""##########"#######"#############"###"######""##########"######################"###"##"#############""#####"##########"############"################""####""#"##############"#############"#############""#######"####################"######################"######"##"##############################"##########"########################################"#############"######"###"##"################"#"#"###############"#"#########################"######"######################################""#############################"##"############"#############"######"#############"########""####################################"####""###########"####################"################"###############################"###################"#########"###"#########"#####################"####################################"############"##################################"######"###############"##"#######################"#####################"#"#####"############"##################################"############"################"######"###########"####"####"#"##################################""##############"#####################################"#"#################################"###########"#"##########"#########"########"###"K1313_E3_Crusea_calocephala_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z-9EUi%4BNVYXTNJGIMT[bjqy2COW]dn{v]>tK%\6(Gn7g)b$'!lU@*eD!pH}rh^TJA:5323579997531124553/+(&%&&%#! 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) '*+ % #- %)$*E3.17#90()#!"R=).(2*.""#/6 &F>>:#62K!(RNH ,5" ! & Z($ ! %#8-278.&-!1) (#)-5B*C:X1E9&'+")1#,(1:)N1,E(H'5-N:!0,%/"@&I0")l(N>=j;'58#*9N1-76 ,97I2-;$>|7]).D6FMAA"`?/AQYM{p?LZ:016bN.</M&&V8UP]fGUeN01 q(bPB-(?Y@4j0tf='=LZ&0HWzUPOxHv ibrRJK2tK/PCD-rfN;8BCp?2V6O  A5-GAAGTGTTGTCTCGAGGTTGTGATGAGAACACCTAATCACATAATATACTGCCGTATAACGCAACCGATATACTGATATCGTTGCCGCCCTTCGAATCACTTCACCAAGCCGAGCTTCTTCAGGAGGAAGAACCGGCGCCAGATCCGTCGAATCCTCTTCTTGGACGAAGACCACTGGGTAGGCAGGAAGTCCTTTCCCTCGTGATCGATACGGAAGGAGGAGCCATCACCTCGAGACAGGTTCCTGAGGAGGATAACGAAATAGTGGCTGAGGAGCCTATTCCCTAGATCCACAAGGAGGAAGGTCCGGTACTCACAAGGATTCCTTCCTCGGAAGGGAAGGAGGTTTGGTCCGAGTTCCGCTCGTTCCTCCTCCAGGATAAATGAGGACTTTCCCTGCATAAGATTGCATCCTCCGAACGGCATCTCCACGTACTAGTGTTTTGAGATATTTAAAGCACGAGTCCTGTCCTTCCATAGTCAGAAGCACTAATCCATCCCCAAGATGGATATCCGATCCCCACTATTGGAGAGCATGATAAGTATGTCCTCTCTGTACACGTATATAACAAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-18 23:35:57 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt$ASPt$B1Pt#B1Pt#BCTS~PBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?ZTDATA ?ZDATA ?ZS>DATA ?ZҘDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~|LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM>|hP1RL>|dP1WD>|mP2AM>|v P2BA>>zP2RL>|qPBAS>>TkPBAS>>R-PCON>>aPCON>>_PDMFTjPDMFRPLOC>|[%PLOC>|VPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 1RUNT #RUNT  RUNT "Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL##TGSPACA\*SPACR SPACA\*SVER4.0SVER ~ESVER,,/Scal@Scan@TUBEE3Tmpr<UserphARphCHR(phDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt$ASPt$B1Pt#B1Pt#BCTS~PBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?ZTDATA ?ZDATA ?ZS>DATA ?ZҘDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//kGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~|LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM>|hP1RL>|dP1WD>|mP2AM>|v P2BA>>zP2RL>|qPBAS>>TkPBAS>>R-PCON>>aPCON>>_PDMFTjPDMFRPLOC>|[%PLOC>|VPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 1RUNT #RUNT  RUNT "Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL##TGSPACA\*SPACR SPACA\*SVER4.0SVER ~ESVER,,/Scal@Scan@TUBEE3Tmpr<UserphARphCHR(phDYbigphQLcphTRphTR.iTr8p|_1{63_`EHsMT@OmAmDe8HR/D2GcA13woF$1PI?p":*Xq)apMf4S><3$G,lwd-l#frL?*Y\FX`@kjJ}B~xGH$i*8j|]]u.ibuILW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A\&C9":?ZEq1hu$#k:X=<;3On&+VN;172e,qz7|!Y<,h`Fzmj7Ul*8ml{Rc9KiB_ v!(Zq)(Oc:=Gk;0|5Q1V?|&@8ja:3AGXS<< xrTW?iMAkc PkIp?H^Poa#]}Ko)6V8JX|K2-!;9xxsWD@|DmOY,f *"49J_'JKxwQ4fC\{lKd#8 UC;2i$@jEl0\+d{II.tAS_w3wAc3[{Vz^`aWyoAN+$0EHW- 7@o*.AQ>U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JUx2ffU"&6,2SayX,8JThnbi2dP?l~Wo]yi-IX/TI@fsQq&Esk 1X$!}5MfiYP[yy] 2,\auBDD"K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLAEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt$ASPt$AUDTB1Pt#B1Pt#BCTS~PBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?ZTDATA ?ZDATA ?ZS>DATA ?ZҘDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//kGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~|LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM>|hP1RL>|dP1WD>|mP2AM>|v P2BA>>zP2RL>|qPBAS>>TkPBAS>>R-PCON>>aPCON>>_PDMFTjPDMFRPLOC>|[%PLOC>|VPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 1RUNT #RUNT  RUNT "Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL##TGSPACA\*SPACR SPACA\*SVER4.0SVER ~ESVER,,/Scal@Scan@TUBEE3Tmpr<UserphARphCHR(phDYbigphQLcphTRphTR