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D ( A = / 8 - D D I C > 7 1 L A P = , : C < . < G > ( 1 K K 5 / % ] I E 9 F > : > # : X 7 8 C K A c e u K @ ? 2 3 8 S C H S F E ? 1 8 2 ( > 5 > S 9 $ : = 5 M F F P C 8 . D @ ; A < G < 5 = > 7 T I a 6 [ I T N ? / C F - = T = T Q a R 5 O L O A V U D Q M a > , ; R X G ; A Y @ " D 9 7 ) @ D / ' ? ? 5 N $ < M 9 C 6 3 @ = 4 4 R G B ; ? 3 3 C A G ? K F < A G @ 4 X B V T E B 6 n [ K _ m b h X U k d D < T C O E < q c Y [ l ` K L C b i ^ e S b d V g ] ^ ^ X _ D 8 ; C M 9 D ] [ U O J @ < 8 I R Y $ P J = H Q ; V 1 ( I g L H ? < = ) B E G / > \ N V 6 2 K ? 7 B * @ [ G ? \ P R M W 9 % Q B E P 8 Z L 1 c M l ] Q g P D U K i } Y T o h j w x a _ W ` I l Z a B I 0 _ h P Q t [ p k T b v P l n n ^ X } d k h u y O ^ o e m k a j r k s u ` x | Y k ` } l m t f f f l ` _ J V f N X ^ x m W l J L J } e J ] [ o [ y _ b h g y U _ k o o ^ l f r Y s l j m e Q Z a i t l c c ] u N _ I X n \ k [ U d V n j x | u m s p n f X p q e p x | _ a a z n V l : c u w h i b Q f | ` R V L Q W L E V k Z < c y N p k R x ^ u X O [ h _ U \ h Y R V S h \ R [ b Z r u d ` ^ j X m f z g g r m u n ] h V K I B I a X a M X ; T ` ^ \ N H G I U ` d ^ X R o d O F L I : C 3 > 0 J & E > ( G 0 B A C A ^ * B E 0 2 C H 4 V Z W O R A 5 / C > K A E E F 1 7 K J N W > ? ? G 5 A , : B 5 L Q g j X X Z B 5 8 B P Y Z 9 I b R C R : T W I ] M H Z X M E O = \ T b M O h O @ V O h X = [ O K Q T E G ` # L L G H W = 9 Y Q > C 6 B E D X c H F H V 6 S = O D U L K M @ U = W T < + U ; J _ h X L V S D 7 = a " > 4 T Z K U G O 1 B < + 7 _ , G T F V Z E Y R ) > < O ? e S S C Q b P J H r g O B W \ j _ W c ; b K ] G N W P ] X \ b K ` Y F H f { c k u D \ X W k j f e Y d J U Q j k d Q [ U L [ X g ` u i X ] M [ e b O \ U X P \ D ` 8 M Z N C R L H > U Z [ 9 a S b I : S R U \ A a a k } D Q M K Z j i E A 9 E W P h [ W l h y i } ] { L j k k t j t y { q p x c U I W h I \ \ ? Z ] f ] | | | p | w g q Z ; a a b l f m j u Z n z b ^ z { | q s n o i h j q x e h w | ~ _ s z z u ~ l x q i j N ^ U U w R N [ D @ O _ L Y \ h Y v Q 9 K v V K G X C 1 C F O R Q s T F 5 F C P U S 5 1 > j ] U M ; N O ' @ K M ; B N ] ] B ^ M \ K v J S d m j g Y V [ a t _ X k m h \ R I a q u u n n | j ] n e V o e y n ] h ] X E _ P Z h E J H > M \ J J q o n R v i \ i k ` e a V } u p , N } Z Y ] f t h _ { d e b ~ f v u Q a n M b k p s v h n { k v _ k X o e ` P ` O A e N L A L ] F U b a c Y I H 8 V @ J C R [ b } d X f ^ b V a K ` S P f w w h e l _ W ^ y c m ] W k M V X a [ P e W L T I a i W V \ n f k e e j R F Q [ \ y | U z O { m h h g Q U Q [ k i ] o Q T m T w ] e C n { a e o v c n z d q k g h | l h c d { i u x p { x i } \ v O b d t u Y { } Z p { v k r | ~ o t m | y w s _ V ~ u v J i I x m c h y h p r f { O o w | x s z t v i z t | x } } } z ~ ~ o | %7%+ );KL^B@ex~\RLG | u y ] y d l l u v p t h P Z l h N e f N q w Z r d t g l I o c X g k e V K S g X Y U e ^ a U a r X Z Z d c | { O c v l r g | ` ] g ` n w ] s X c H q ^ V c q r I O h W U k b i P S f N j \ S d R _ ^ \ X _ c o b l u C R [ m l l \ N u j I > ` ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1978e1d7dc5243d18026024c2950bf36Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-07_18-20_0151 3130Seq 12866 12866_Steier_K1340_rbcLR jmadera 96-Well K1340_B11_rbcLRKB_3730_POP7_BDTv3.mobSeqPop7z A A B B B A @ ? > = > @ A B B @ = ; ; ; = > = ; 8 6 6 8 : : 6 / & " - : G O R P K G E D C @ 9 / $ $ 0 < E H E > 5 - ) ) - 2 7 : ; : : = @ C B < 3 ( ! ! & . 4 3 + / ; B C ? 9 4 2 3 5 8 9 : : : ; < ? B E G F B ; 2 + * 1 @ Q _ e b Y N G F L T Y Y S J C C J W e o s p h _ X T S U W Y \ a g n s v v u v } .h
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, 0 * x s@7AAPCA8KB.bcpKB_3730_POP7_BDTv3.mobtermAAGTTTGGATGGGCGCTGAGGTGTGGCCAATTTTGGAGACGGCCCATTTTGGCTTGTTGTCCTCCCAGTAGGGGCCGTTTTTAAAGTGCTGGTTTTACCTAACCCCCGAAATCCCCCTTTTTAAGAGACAGAAGTTTGGATGGGCGCTGAGGTGTGGCCAATTTTGGAGACGGCCCATTTTGGCTTGTTGTCCTCCCAGTAGGGGCCGTTTTTAAAGTGCTGGTTTTACCTAACCCCCGAAATCCCCCTTTTTAAGAGACAG ) < K [ m { (/8CJRgm{+2>KWjw!5AJYcoz %.;DR\fu ,9AP[ft~ !+6>JXe ) < K [ m { (/8CJRgm{+2>KWjw!5AJYcoz %.;DR\fu ,9AP[ft~ !+6>JXe
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;qdX(ayTCTAACTTTGAATTTTGTTTTGTCAAAATTAACCTACTCTAAGTATCAAATCGCCACCCGAACAGACACTAAACAACCCCAATGGTGCTCTCCCACCAAGTCTAGTTCGCCCGGGGTCCCCATGAGAGACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-07 19:51:16 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D * CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G ,H DATA # G s DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ] LAST ~ ~
: LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS N P1AM P1RL P1WD P2AM P2BA P2RL
PBAS PBAS PCON PCON PDMF ~ PDMF e PLOC PLOC PSZE ` PTYP " PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 17 RUNT ( RUNT / Rate RunN " " S/N% F SCAN 3 SMED
SMLt SMPL n SPAC M SPAC ^ SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B11 Tmpr < User phAR phCH | phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D * CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G ,H DATA # G s DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ] LAST ~ ~
: LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS N P1AM P1RL P1WD P2AM P2BA P2RL
PBAS PBAS PCON PCON PDMF ~ PDMF e PLOC PLOC PSZE ` PTYP " PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 17 RUNT ( RUNT / Rate RunN " " S/N% F SCAN 3 SMED
SMLt SMPL n SPAC M SPAC ^ SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B11 Tmpr < User phAR phCH | phDY big phQL c phTR phTR qCfkNR(Bj'~;Hk@*w&RA#] lM6[/:){hV(wX>?7u%#F7Np1mj;RJJ4R,9X PMF9zH'zAuR)x|ec{PG6x|W"OKOfx&i1PBEe>2flV:2/dmm YJX?/WZ#}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU#_[A(C"W(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYqB %T@t:/GR:!iugUFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdAko+6HtsG,XQYp)WgP!gFS]T'_C:rF(5,f *"