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P ,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3SSSLLcjj""",,+544==CEEMNNVVV^^]eeemllsts{zz<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 328c3946bc7b472ea1d850c50d94d331Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_21-23_21173130Seq1027110271_Lehman_K1358_SNPMEX_rbclfjmadera96-WellVeronica_persica_K1358KB_3730_POP7_BDTv3.mobSeqPop7z!A[o.Gb>a1EXk|{oeZOB1ui_YX[_bb^VLA6+kA`? .Ih?p "!~tk`R?)vT2}naXSOMJHFDDDEFEC@<8543346788865311134679;?EMWdtH{Iwi;g5 1jHPvvV1  9LTSI<0+.:McxnA_f*8~(.@*\SnM;1-)'$#"#$$%$#! 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N96D;0QH:H)<Z`\%GN70'HQ)])a\?U@ C2>>W"<O2-4Id-=c!a?.065 2&?"* E> Y*ME V:,=.`'V%).  $Z6^ *D?UT/B*@bAW])[S`X6 $:JI%W+P/ -R4.& X 'H ],*Nb[2BHNT!U'4G<c.T?1;82 L700!#4Q[<N ;I<X)6PX[a/FK8')O XD^7`JN /)9- !$ K ELB280N03*K ,9: 59D8!%"/L#* >NV1% a=V<%:K!E+- *LV1U@ NI;a.$\0^[P_N#*[>S ?bIDE$32 XXW-.SI4.NYMZL1 'NV OL^*LI#U1);7%JDLJ9Y7G#<G![5*W 2@KT.PF1 [FC$]>Vb`:9-_ ,$3L*(? =W(H 0I/-$$.(@Y10> D]&"L%KQEaCY) )A0)D 2YQa %#34[rCE}3cv {@+VUE}m)!zQ/zB"rW;oA,g_va'q3bjo- ,6ASlY| o l+(I|(@(Q*>HQqk3;Pk[t!QMl(f~1 4QiuSb[-0Kj6yp R+&@7aJ,<zLa6M_iX*noo` 3mFeo6I(nZYp!5'Xa y4N4*Wl4}mh 6+ex1Ls9\0,DmA={0ZG.M)3IuVz+y\, {1)efd|b=X6\R:i?SG/Z'4)pE1\ ! d "  ,if5g}.dcV^WXMSPBK9H=>72.9/5 ""  '"                               *%*&"$#,/!"3*#-)+,+&-.2-&&-6*:)5782@&'.1(31+64H47.*-16084:*:54=2?198AHB??I;<KD<<HGHJGI?fDDINU<OABOAFLS8IMPKJKdCO_6HI#777?=7=777=::=Bohp|sp,x8'@,pdIN138`SdB gw<8,$Zp|wyVLE/XGy<d'\^_$d~P8d/ddk# ,@8ys8d,8~, R msL8^drIRrd7dm[Fovrkd Joh8MIMpp$p{},T,pl.j&-kX jnRp*wQ=p1php2p;i?<pzpLpTk8gt~SI6o%on?d8.\F7S5+#(%*ur8M880`(-a}3%j+?)!!-"$!#'3 &V2=>!%+/'% !$  &4&*& ) $  5(!!%%'#"=*".$!%"&'+*' &!3%2 (!'"$(1"$"$+.#=3 0!&"&%' 8,-7!6*@%& -4*## &"3!+&,%! +!!%,7t<21/6#+H3($ 66!:! #8A(&,!.>8_#u38(" *)"(-+g !& /:1F)Kp86 #3"$.=#%Q0 2G$!% FZ2.(;N;" "5IB<=%+86LM.-@/I5i*$A`:+P$4N83-v%0  [GAGAATGGGGTATAACAGCTTGTGATGAGTGCACTTACCCACATAATATTCTGCAGTACAAGGCAACGGATACACCTGTATCCGTGCCTTCCTTCGAGGTCATTCACCAGCCAGCCCTATATAAGGAGGAAGAAGCTCCGCTAGAGCCGGAGTATCCTCCTAATGTTCGAAGACTGATGTGAAGAGCGGTTGAAACTACCTTCGTGGTCAACAGGGAAAGAGAATCCTTTATCTAGCGGTCCCTTGCAGAGCAACAGAACGAGGTATCCAACGAAGCTAACTTTCGATAGATGAAAAAGGAGGACTGGGAAACTAGGGTATAAACTCCTTTAGTAGAAGGTGAGGGGGTAGAATCCGCTTTGCGCACTCTCTTCCTGAAGGAGAAGAAAGGATTTGCGGCTAAGAAAATGGTTACCTTCGCCGGTTTTATTTTCAGGCTGAAAAAGAGAGATATTGGAACTTCGAATACCCTTCTTCTGTGGTCATAAACTAGAATGTTTATGGACTTTGAATATCCTATGGCCTCCTCTAGAGAATATAATAAGGATGTACTCCCTGAACCGGATATAAACAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 22:54:52 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSUBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (Q6DATA (Q65DATA (Q6DATA (Q6DDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~TbLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS)P1AM?~@+P1RL?~;P1WD?~DP2AM?~MP2BA??R#P2RL?~I'PBAS??+PBAS??)PCON??9nPCON??7/PDMFPDMF)PLOC?~2PLOC?~.3PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%)zSCAN3SMED SMLtSMPLkSPACAd SPAC)SPACAd SVER4.0SVER USVER,,fScal@Scan3TUBEE10Tmpr<UserphARphCH)phDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSUBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (Q6DATA (Q65DATA (Q6DATA (Q6DDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabdFFVocd5FWO_GATCFeat//dGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~TbLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS)P1AM?~@+P1RL?~;P1WD?~DP2AM?~MP2BA??R#P2RL?~I'PBAS??+PBAS??)PCON??9nPCON??7/PDMFPDMF)PLOC?~2PLOC?~.3PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%)zSCAN3SMED SMLtSMPLkSPACAd SPAC)SPACAd SVER4.0SVER USVER,,fScal@Scan3TUBEE10Tmpr<UserphARphCH)phDYbigphQLcphTRphTRa|cty8JMB*u $O\4I*[DeNq,P+C#Lc93"~zj;N&`,'Kym&FB2p$,%9tWa-cZ,\|b=-j%`3nXv^.PZeOY ~?p96UI//`c!sH3p{ ]G.-RRC(vJ4;N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO>^7ghRS|6dc+GHO5QqEi>o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbW)WjmH_T+hSZy&m3HPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'4MYbC*TH]pD,VS&sF{LbzMiWJ+yN"J9'!hvRdZY`X {1tMo])} ~T>G$~I!|spKa3gDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7~.<{[t*`Q<,{AmXXt;*hZyP4)@Dx`575^{ e.L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bf+mbz9ehA.M}d@yV+@CdU4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKB_,qz74*le_M%~'pEA({]/AT^ogqlp,4lpy]AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDT||rYB1PtB1PtBCTSUBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (Q6DATA (Q65DATA (Q6DATA (Q6DDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabdFFVocd5FWO_GATCFeat//dGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~TbLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS)P1AM?~@+P1RL?~;P1WD?~DP2AM?~MP2BA??R#P2RL?~I'PBAS??+PBAS??)PCON??9nPCON??7/PDMFPDMF)PLOC?~2PLOC?~.3PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%)zSCAN3SMED SMLtSMPLkSPACAd SPAC)SPACAd SVER4.0SVER USVER,,fScal@Scan3TUBEE10Tmpr<UserphARphCH)phDYbigphQLcphTRphTR