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  !$"          P ,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3SSSLLcjj""",,+544==CEEMNNVVV^^]eeemllsts{zz<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 798afaadb3e74f7fb0b6cc1d39019d52Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_21-23_21173130Seq1027110271_Lehman_K1358_SNPMEX_rbclfjmadera96-WellSambucus_nigra_K1358KB_3730_POP7_BDTv3.mobSeqPop7z )A_}#0=M]n| #1@Qapz{rgYI5iD!qZH:1,+.6AQf~7b:[xzi[ND:/#z[:iAui`ZWVUSOJD?;9863/*%! 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H*W;JHE(IZ_879.a^*9W/9I4T'RL_#>^&DPLRW+U651)cSR"9=%VH'QK=,>1J ( b+JFU*0c^$AQX-#^b)4H354,S;Z $1\W9"JKRN 8_2XMM/< 1ZARD F&A&%cX&#[@O*#K' !Y5.0#.LR,N`], ],.3 J+@3d-=/!K5 I21482KO6$3 $=D,D4^ZZ<2WK%&3NP:W4Y*]!:=P5 &4Hba)]_,d.71 A2ENQK@;%UB6@.GI,'2%>0F,]"<E^"XW@>-P#5#@'"SQLR>%%>AJ+:CJ&D ?UGXA2T]N'03;;I:66GO?K*C 5R1V8\^8 \12P4-=H$DN3<GG_9Q:/5-@[4Q\<L@FGM_P!'@9`1Y?>1&,RFWT@%U[AcK4H7Pb?\'=+APTM]c7Z:R;B"(2Y< DE#.K$GP N/RIV"!SG`#FU))/!2C'.!'6G2G=C2L6E8 V1nk`BT-C2zrJl/ZV!;'|I }aS]_VfLAdY$o>=*JyO%h%ycO5dtb UCAa_]_1*LQpsqTceE<{gn=4(d \%gV*% Lan7P*!IW;!Qmk)kbME@L ~o2-f(X/g 5'C)0"^ bC52#,+j->qSK]P!+CNLi\a($xSYfL0Wx T[):[bCfh.?B.GM($Id%z[:c}%g-y2VmGmPe FC' o GZ 5D H " >O1 (HQ9HOgm)\X[fQLdUQOADB6MA7L0@KO?%++)(*%(1('                                                           +   +      ((  $*  %%.--1!/#&=%('!.-(%$02O+-1*,/17-$0,%-;;;0!1'=/+0A&2GD2G0P5f;>5/?[[+E@47A@L>=CFMFHRR8GFR]SX[>EII?LMXU[an{nMPG^SsiSPRTXdSiE]mktLw[p]ny^hwfekdlxl|jbn=CnlcTx|Oxui9gYM>|Gs9aNgNvv=\Z1MMMiZMMZMMZMMZZZZZZd] fpud:p Np+p5NBUdGpdop]p'_R,x8vpd/ykuI-QpP,88dip)d|(m48&>,[8%LQv&Bvu(zP8; R<AKzO NK/p3S>x%p+dNp7X`d&:/{!pp,pa:818W`$pphdapp jv6qj/I%2B>`g>C&dmZMYiPJ7"3Jc ']Eo.f"'~+ %+." FEGR !<$'& "$! %#  0(*! #"( '% #"4'6"!+!7$&#$.%"%""2$'"!)',&!) 7 /!B%58"$0)+8((-D*&0'%)((7/J&5 -!%$2 >?(K>S!!&K)-_),&!#49F+&2"*D92Q,YJ(N5L8M1.)e3>4!AZB-+/(/GI(6.T""1? +: -)-8A.QV0B:14O65b%02-.7F=5_v0=t3)# {zP#5@5Co9LmWHkVU95<E3nIjVQh.X)BhL?6PC2zF|WNOr'(J2Oe&FL>HAtNn,2RL<lBpH^m,wD<>u  !PpdAAGATTGGTTTCACGAGGTTGTGATGATAACACCTAGCCACATAATATACTGGAGAATAAGGCAACGGATACACCTATATCGTCGCCGTACTTAGAATCACTTCTCCAAGCAGCCCTACATCAGGAGGAGGACCCGACGGAAGAGCCGTCGTATCCTCTTATTGCACGAAGACTACTGTGTACACAGGTCGACCCTTCATTCCTTATCGCTACGGAAAGCGACTCCTTCTCATCGAGTTCGGTTGAAGAGGAGTCAAACGAAATACCTCCTAAGGCGCCCATTCCACCCTTCACACAGGAGGAAGGACCGATACGGACATAACTTCCTTCCGTGCATGGTGAGTAAGTTAGCTCGGATTTCCGCGCTGTCTACCTCAAGGATATAAGAGTACTTCCCCCTCCTATGATTTTTAAATACGAAGGTTTTCTTATTGGATGCGAACTGAGAGATATGTAATGCACTAGTACCGTCGTTTTGCGCCCTTATGCAATAATCCATCCGTACTTTTGATATCTTATCCGCAATTCAGTAGAGCAGAATAAGTATGTCTTCTCCGCACTCGGAGAATAACAATTCCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 22:54:06 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSSBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QPDATA (QP5BDATA (QPDATA (QPֆDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~RoLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS'0P1AMD> P1RLD9P1WDDBP2AMDKP2BADDP+P2RLDGPBASDD)PBASDD'cPCONDD7?PCONDD4PDMFPDMF'GPLOCD0sPLOCD+PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%'(SCAN3SMED SMLtSMPLkSPACAeWSPAC'@SPACAeWSVER4.0SVER SSVER,,fScal@Scan3TUBEG3Tmpr<UserphARphCH'^phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSSBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QPDATA (QP5BDATA (QPDATA (QPֆDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabbSFVocbBFWO_GATCFeat//bGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~RoLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS'0P1AMD> P1RLD9P1WDDBP2AMDKP2BADDP+P2RLDGPBASDD)PBASDD'cPCONDD7?PCONDD4PDMFPDMF'GPLOCD0sPLOCD+PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%'(SCAN3SMED SMLtSMPLkSPACAeWSPAC'@SPACAeWSVER4.0SVER SSVER,,fScal@Scan3TUBEG3Tmpr<UserphARphCH'^phDYbigphQLcphTRphTR(w}Uzrla@O(LxC[>({]>REd7gbOHG'~[ABMNT@bxj[Dbt6XhVx9zG@[76gg`qC+lkfc40-fKbVU}MS{p[v[!s.#g;X6YM'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ-,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM1voDGG5)ljD_n?Kf]4'Uw\Pcc{\A_}$A>AR5Z(>B|::zWTR0Xwt2)lOY^k42g|5 x*.JIc\B:!VB_'eLgo5V"W1Qv&4MM`$M(P@>/D?*Uq$[-o9D4G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H%R7R@(l\fo!(Cn [v1:q?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziP-2ZE<}Q6odPz%X;Frxp/Ygn P1RLD9P1WDDBP2AMDKP2BADDP+P2RLDGPBASDD)PBASDD'cPCONDD7?PCONDD4PDMFPDMF'GPLOCD0sPLOCD+PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%'(SCAN3SMED SMLtSMPLkSPACAeWSPAC'@SPACAeWSVER4.0SVER SSVER,,fScal@Scan3TUBEG3Tmpr<UserphARphCH'^phDYbigphQLcphTRphTR