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(!"'(& 7 &)!'         &    P ,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3SSSLLcjj""",,+544==CEEMNNVVV^^]eeemllsts{zz<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a75f0b39024f4216919432bd2b1b94c1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_21-23_21173130Seq1027110271_Lehman_K1358_SNPMEX_rbclfjmadera96-WellPsilotum_sp_K1358KB_3730_POP7_BDTv3.mobSeqPop7z %=[x!+2;FTet~tjbZRI=-~iUB0{uplifc_[XVTTTTTTTTUX]dmx?eAtL} ! iYQMIA0vK!|R)~skc\UQNMNOONLJHFEDB@>=;98631.,+++,-.//0149@HQ[hz/_-dt>Rp[MFCCFIMQV\fv>}uF;[`L%v: E*9. %[O Tk./0kL8-('*.379986410.,*'$ "(0:H\y\8t{O+!GG OuvNS6' #'+.02221/,(#   &H{o"d|GEc7    G5^ W7b+          2hYU"? #(+,*'#   "&)**'".h9R <=P   3k8Lye>?;pF / (-000/-*&    8<\`  %'$    !C}(cf2   Mz9[FW   Oje* j;-gxO  8aQ[N)! ${      2&& 0GDr_+l8 E tJNl,            K,m1U  6v?,]a)9x'   !^/81I ## Mp# 8/1HA1%UuC  <\/,eS  $`xgPLHBH #"xJa   /& ?C@ AmH  K8bSe7Vn(      7RZB  Mi;B`=']7cX$     7^9,XzB     YBiA eS~q 3    /G!{l "'%   5+~c_ K\@]9bx.   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QokU= g HPB _ L@!Q}r_~aj0_lo_JOFFPA<87:7/--2,(*!x6&                         % $$#)   "'&%@2 < /&& B!5//66;*=0+')20;8<.7497',4A7@>C#-/F60<;2?>?96GK;9O9WNCNLECMNOFQOBDMJPPPLdLAVrW^FMOSUP}bQ^QYSH]@_>T\ELKVWJPf?O@4<888WHHHHH8H8HT8HHHHHpppp5<d IpI$.ppaOj9TT pN[[h|gdd{S)d8~NdS)8d.} K&%@j!d4 Ddu,pB`L,+ID7d|/ppd(.~8sS.)q6 15ry`a'ZPJ  tpr80&00>t$pU3&Mp*#*n$c.8zW,gp 9DZg1I{J55a8ACDzd .eH$$3Z"Ed[D @VYTBJ"/9^=\~X'G!0' "')%+\#' =:-W+-.,& ,& "3!5 -+!# $ + '*/%$& "&."(-%),,!.#$%3")7+ %*  "%2%,:(&" 6%/N&"(/*/>&$$# &;/'-.1!&!&7F 4D" )2"04-(!3-%#0+R22'_&U7B3&3$$42+!<&=/# >, 'DSK}+7+M4(24O !]f0B&"$4=$0'2 ?4,6.F! ls,WJ+,*)/[b 7J*&7)FNE^:#N>.B4eA48.:7/CQ47/F<);a}s<>31V^B9n5X#>RW'( zGAGACTGTTATAACGAGGTTGTGATAATAATAAATAGACATATAATATAGGTGCTAATATGGAAACAGAGACACCGATATTGGTGCCGTGACTCGAAACAATTCAGCATCCCGCCCAATGGAAGAAGGAGGCAGCTCCACTAGATCCGAAGAATCCTTCAATTGGGCGAACAACAATGTGTAGGGCGGATGAACCAACAGAGTTGGTCACCACGAAAGGTGAATAGGTTGGAAAGGGACCCGTGGCAAAGGAGTCCAATGGGATAATTTCCAAGGCGCATATTTGGTTTTTCTCCCCGAAGGCAGGGGCAATTACCATACAATCTCCTTTGGTAGCAAGGAAGGGGGTTGAAACCTAAGACCCAGCTGTCCTCCAAGAGGATAAAGAAGGATTTCTTGCTAATAAACGGACTTGCATCCCCAGGTTTATTTCCGGGTGGGAAAAGGGAGATATTTAAACTAGGGATACCCTTGTTCCATACTTATAAACAACCACCTTACGGGAATATTGATATACTATGGCCAACACTATAGAGCTGCATAAGGATGTACTCCCTGAACCGGAGTTTTATACAATTCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 22:54:43 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSXBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QDATA (Q6eDATA (Q-DATA (QDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE>LAST~~WLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS+P1AMDBP1RLD>P1WDDG(P2AMDP8P2BADDTP2RLDKPBASDD.<PBASDD+PCONDD;PCONDD9PDMF}PDMF+PLOCD5PLOCD0PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%+SCAN3SMED SMLtSMPLkSPACAbMSPAC+SPACAbMSVER4.0SVER XSVER,,fScal@Scan3TUBEB8Tmpr<UserphARphCH+phDYbigphQLcphTRphTR/This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSXBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QDATA (Q6eDATA (Q-DATA (QDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabfFVocfFWO_GATCFeat//fGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE>LAST~~WLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS+P1AMDBP1RLD>P1WDDG(P2AMDP8P2BADDTP2RLDKPBASDD.<PBASDD+PCONDD;PCONDD9PDMF}PDMF+PLOCD5PLOCD0PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%+SCAN3SMED SMLtSMPLkSPACAbMSPAC+SPACAbMSVER4.0SVER XSVER,,fScal@Scan3TUBEB8Tmpr<UserphARphCH+phDYbigphQLcphTRphTRMr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;N }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_VuW)qhA]D9SPQtm9Kt=#&qsKFMrRU)>`Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9} P,hz9_"#kjFO-U[ds=:I1On(`"]/r;H>.#|P0Y,\I/P4);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilbZ]"Po1Cd}F<_`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQLAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDTrrtB1PtB1PtBCTSXBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QDATA (Q6eDATA (Q-DATA (QDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabfFVocfFWO_GATCFeat//fGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE>LAST~~WLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS+P1AMDBP1RLD>P1WDDG(P2AMDP8P2BADDTP2RLDKPBASDD.<PBASDD+PCONDD;PCONDD9PDMF}PDMF+PLOCD5PLOCD0PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%+SCAN3SMED SMLtSMPLkSPACAbMSPAC+SPACAbMSVER4.0SVER XSVER,,fScal@Scan3TUBEB8Tmpr<UserphARphCH+phDYbigphQLcphTRphTR