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P ,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3SSSLLcjj""",,+544==CEEMNNVVV^^]eeemllsts{zz<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1fd22ca767524295b3c6e1964fdb316cSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_21-23_21173130Seq1027110271_Lehman_K1358_SNPMEX_rbclfjmadera96-WellNicotiana_tabacum_K1358KB_3730_POP7_BDTv3.mobSeqPop7z<Ymy(5CRanx~}|}$7HXepyucK/b:pQ7 /Qt)S!8GORPKD:- zm\H3 {Y7sg_ZWTROMJIHHIIHFC?;864320-*(&&&()+,,,,-.026:@GPZgxJy1YzpL Nte[UQONOPRV]hv)jo6 #$yB6o  f:D 6lb9h <uO5(""$%%#! 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(ji`79gnO C 5Ug=!2NkL_{fTzWF:5XV8pkya` M ]rr8Y!.Py() 0&uJA[ j9qa1aNrWe#g{5`c+Sb09xiuFJ.6M'M DCRAJc .Z Hm  Yo;/L6>sgj@g]Z[]LMNQEHDCA;;779."%%;!$$                  !  $ "  2+a% "%!.)..7(', '!*('0/,0/&!28380%.7+7)06/991>33F8</-8198?9>5<7+B;EN@EJEC@MTBENKQcGL@I>bVDkCCKGDHMIOVEMHR\Q TR:LM+<<CC;<<<;<;<<;;x.ppj  8>\,v8Dd(?8C#8&pPvl.T*8m 8d8-p; ,rt5w1I-dj_5 %d dT1y\l="1/jHd]%,d[grp2FCPLZ{;u~,qM,M@dp:%pp0aq{V9dY4U88K8JpYL3vGppApdS\p ;v;. {K\po;9J8GTN:vi';QLm-2 &<W\M\'Y }M,v4 '!RKMR**%$  %   (!!"  "  " &%'"#" &#$ ) 3' . '#&( $!2'$+0 #"*.)&(!#&$0"<* 63$%"$)/,23'(3(%+ $+7+9(-;Ov G ?.##+)!*.AU&!*!(4-*:=5B3.$ .!+/51E.&##&!( /8(W<9CG.9LGP7A!58*G#898.^Xy&3Y{,,L68[@?BD_8 5wP9$'RGAATGTGTTATATCGAGGTTGTGATGAGAACACTTAATCACATAATATACGTCCGAACAACCCAACGAATACACCTATATAGTTGCCTTAATTAGAATCTCTTCTCCATCCAGCCCTACAAAAGGAGGAAGAATCGGCGCAAGATCCGGAGTATCCTCCTATTGCACAAAGACCAATGGGTAGGGAGGTAGAAACTACCTTCATGATCAACACGGAAAGAGAAGCCTTCGCCTAGAGACTGGTGGTAGAGGGGGACAACGAGATAATGAAGAAGGCGCCCATTCCAGAGTTCACAAAGGAGGAAGGTCTGATTACGAGATGAATTCCTTTAGTGGAAGGGAAGGAGGTTTAATCCGAGTTCCGCGCTCTCTTCCTGAAGGAGAAACGAGCATTTCTTCCTAAGAAAATCCTAAAATTCGAAGGTTTTCTTCTTAGATTCGAAGAGGGAGATATTTAAAGCACGAATACCCTCCTTTTCCGGTTATAAACACCAATCCATCCGGACTTTTGGTATCCTATCCCCACCTCCATAGAGCCGAATAAGTATGTCCTCTCTGTACCGTATAAATACAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 22:54:53 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSVBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QXDATA (QX5DATA (QXSDATA (QXثDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~TLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS* P1AM@@P1RL@<>P1WD@E>P2AM@N>P2BA@@RP2RL@IPBAS@@,~PBAS@@*>PCON@@9PCON@@7PDMFPDMF*"PLOC@3>PLOC@.PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%*SCAN3SMED SMLtSMPLkSPACAcUSPAC*SPACAcUSVER4.0SVER V|SVER,,fScal@Scan3TUBED9Tmpr<UserphARphCH*9phDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSVBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QXDATA (QX5DATA (QXSDATA (QXثDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabdFVocdFWO_GATCFeat//dGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~TLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS* P1AM@@P1RL@<>P1WD@E>P2AM@N>P2BA@@RP2RL@IPBAS@@,~PBAS@@*>PCON@@9PCON@@7PDMFPDMF*"PLOC@3>PLOC@.PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%*SCAN3SMED SMLtSMPLkSPACAcUSPAC*SPACAcUSVER4.0SVER V|SVER,,fScal@Scan3TUBED9Tmpr<UserphARphCH*9phDYbigphQLcphTRphTRaB$T9F_z"\=QtAN-GQ6)lI PK&'Kxf]$bN8O2Rz7}062sXaA=&@X<1p3QLT1;g8kY+-^m]ZOQ>\G`HK_^_<~P[Z66YM'KvT+F4$U]]ReL#(79pA$den?R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb_v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r3oJ6l&O`x Pz><3&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hygS4-X;i+-'+1T:o5Q*oc0c3\EI`N^vp5cM_1h\j[<63*7wJ7{_O6?@dsgbk1^>7(FTc%{M*AqbEdG9}j~:HzpWI23Z;|?921NMhh:OrJHM=*bgk_8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPyr{;qe&Ker"&O93s'!byH1V/&`awv x[.+}H2(sxM\UGpz6IP!gFS]T'_C:rF(5,f *"`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDT~~rB1PtB1PtBCTSVBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (QXDATA (QX5DATA (QXSDATA (QXثDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabdFVocdFWO_GATCFeat//dGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~TLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS* P1AM@@P1RL@<>P1WD@E>P2AM@N>P2BA@@RP2RL@IPBAS@@,~PBAS@@*>PCON@@9PCON@@7PDMFPDMF*"PLOC@3>PLOC@.PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 0RUNT 5'RUNT .RUNT 4)Rate RunN""S/N%*SCAN3SMED SMLtSMPLkSPACAcUSPAC*SPACAcUSVER4.0SVER V|SVER,,fScal@Scan3TUBED9Tmpr<UserphARphCH*9phDYbigphQLcphTRphTR