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S S S K K V j j """+++544==@EFLNNUVV^^^eeemmmtss{{z < < < < < < < < < < < < < < < < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e470a8f5dee942cd971cd6d92a57303dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_19-52_2116 3130Seq 10270 10270_Lehman_K1357_SNPMEX_rbclr jmadera 96-Well Ricinus_communis_K1357KB_3730_POP7_BDTv3.mobSeqPop7z 0Lt 7`9~P
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' 5 D E D J V ; c / + : G 0 ? 5 7 u S a M : A G < F < @ D ( 5 H 0 & B 5 ( * < < 4 , & ( ; ? $ U 5 , ^ U I * b # D $ . . B : R L 1 Y ! - . 3 ( $ # D ! S f @ ? H # 7 M h 6 r & =& @ 8 K > a $ < r s F ? c U + ( = ; ` N T i # ( R \ E K [ V 3 9 " O Q | A t ` y R ( G [ C : Q G 6 < = ? 8 X 4 8 R ^ I ` T k q 1 G E V | O o o N O v 4* P ; T l 0 X X b q a * i ] ; q j w Ts p : s 2 N d b y q 3 W%UR)1Z u q .AAAAATTGGTTTTCACACGGTTGTGATGATAATATTTACCCACATAATATTCATCCGTATAAGGCAACTGATACGCCCATAAAGCCGCCTCGCTTCGAATCACATCTCCATCTCCCCCTAAAAAAGATTGAGCAAGAGCCGCAATAGCCGCCGGAACATCTACATATTCCAAGAGGAATGTGTTGAACGCCCCGCACAACAATCCTTATTCATCTTTAAGACGAAGCTGGCACGCAGAAGGCCGTTTCCGAGGAGGGTAACGGGGGAATGCCTATGTAGCGTAAATCCTTTATCCCCCTTGAGGAAGGGCCGATAGGGAGAACCATACCTTTAATAAAAGGGAAGGAGGTTAGGTCCGATGGCCACGCGGTCCTCCTCTTGAATAGAAGAGTATTTATGCAGCCTATACCTTTAAGGTTCCAAAGTTCCCCTCTTTGGTTCGAGGTTTTATTGATTTACTTCACCCCCATAGTAGTGCGGGGGTTTGGAGCTGCCCTCTAAACCCAAAATTGGGGTTATCCGCCTACTACCCCAGAACACCATATTTATATGGTCTCCGTTGAGGTGAAGATCAATCCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-05-02 21:23:28 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS [ BufT & L Q CTID CTNM ! CTOw A CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA )H R DATA
)H R 7d DATA )H R DATA )H R ܄ DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ Z[ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS / P1AM D E P1RL D Ao P1WD D J P2AM D S P2BA D D X P2RL D O PBAS D D 1 PBAS D D /O PCON D D ?+ PCON D D < PDMF PDMF /3 PLOC D 8_ PLOC D 3 PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 4. RUNT RUNT RUNT Rate RunN " " S/N% / SCAN 3 SMED
SMLt SMPL SPAC Aa SPAC /, SPAC Aa SVER 4.0 SVER [ SVER , , Scal @ Scan 3 TUBE F4 Tmpr < User phAR phCH /J phDY big phQL c phTR phTR - This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS [ BufT & L Q CTID CTNM ! CTOw A CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA )H R DATA
)H R 7d DATA )H R DATA )H R ܄ DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab j? FVoc j. FWO_ GATC Feat / / i GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ Z[ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS / P1AM D E P1RL D Ao P1WD D J P2AM D S P2BA D D X P2RL D O PBAS D D 1 PBAS D D /O PCON D D ?+ PCON D D < PDMF PDMF /3 PLOC D 8_ PLOC D 3 PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 4. RUNT RUNT RUNT Rate RunN " " S/N% / SCAN 3 SMED
SMLt SMPL SPAC Aa SPAC /, SPAC Aa SVER 4.0 SVER [ SVER , , Scal @ Scan 3 TUBE F4 Tmpr < User phAR phCH /J phDY big phQL c phTR phTR mkYbCK)Ul_pD8Jq,./b _.uKxh6O1w^{l{iED>{?oH^}nZ##A+]WJyKPy]Y !T_*|QK:jin'D5?C7bJeoiQ]%[p3}$r&kUV'4MYkftTI|Z{Z[{%;5]U'Q>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W2]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&2$U^X$^(`])>mJGVGkB*Sig]r;a4xG57'!>&7.{M`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFUTT3T&^%FyPC,_,qz7A*k|_Lhv\Z&fIlfpUHSTn$ *D J;LY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d GoS[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0