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S S S K K V j j """+++544==@EFLNNUVV^^^eeemmmtss{{z < < < < < < < < < < < < < < < < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1ac9500a53d74e8b80c9f4f1f1a3b9fbSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_19-52_2116 3130Seq 10270 10270_Lehman_K1357_SNPMEX_rbclr jmadera 96-Well Phenax_mexicanus_K1357KB_3730_POP7_BDTv3.mobSeqPop7z U X ^ h u 0Gc:c&Xc ]L-Du 5 V n y v h T ? . ! yXB;BRg|l?rET|SD]"rP6**8Ok
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BxxRxK I@7,P& ` I } 4 j f 2 / O 4 K k & < & H ' Z E T o 3 M $ : "h d - 9 ! J % 2 ( ' ' _ F ; @ - h > H : } , i 1_& [ 4 G ; . D 6 ) 4 * ) B 3 # , , 1 1 ) / B 9 = - > 9 & 9 : _ H i ! - X 3 - E 8 @ . F . / " 8 ' 5 # 9 5 C P - @ +{ C : @ ^ P 8 W 6 b F K $ H N G & - A = 3 e 3 X > S $ ~ U i v T b X p d z O e r ` 0 < D ) M P H C C s O B ; > / X * D G L 6 ' 1 - b ' 9 B 8 ; & * 4 " 8 i D [ C 8 j : w ; ! c 2 ^ V N X 2 J H 6 H 5 C ` J D r O w O @ W 6 8 6 I z i I V X b 9 p H W 2 B E * W 3 M + > G g \ H p I 5 S / b @ _ [ 7 4 A 6 1 J @ _ c \ F b I Z N M S n F q r M h p . C S 7 > b : y KFAp @ z 5 2 - L 5 Q 8 - m U T > @ ! 2 ttc I X q X B> Y S | ] @ I W A W 4K c7L _ s V y 0 ~ n % rV u V | f bQ !Z%w GGAAGATTGTTGTGAAACGAGGTTGTGATTGATTGTATGTACCCACATAGCACTCAGGCTTATATGTCAACGACTCCGCCCATAAAAGCGCCACAACGCGAATCACGTCACCGGCCCCCCCTAAAAAAGGTTGCTGAATCTATGCTAGATCAAGTCTCTCATCTTCACCTACCCAGACTACAGGGCATACCTTCCCTCACAACAATAAATAGCTACATTGCTATTGAAGCCAATTTGTCTAGGATGATTCTATCCGAGGGAATCCGGAGAACACATAACGAGCATTTAACCCACTTGCACAAGGAGGAAGGGCCGAGAGTGACAACCCCACATTCCCCACCCATCAAGAAAGCAAAGTCTAATTTGCATGTGGTCTATATGTTGCCCAGATATGGATTTCTTCTTCCTATAATGGGACACTTCTAATTTTTTGCAAGGTTGGTTTGAGGTGATCCATATCTGGCGGATCACCGTTTTTTGTGGGGGGTTAGAAGCTCCCCTCCATATTTGCTTTTAATATTCGCTTAGTAACATCGTTGAATCGAACATGTAAGTCATCGTCGTAGAGGATAAAATCATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-05-02 21:24:18 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS Sy BufT & L Q CTID CTNM ! CTOw A CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA (= Pz DATA
(= Pz 5N DATA (= Pz DATA (= Pz B DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE N LAST ~ ~ Q LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS & P1AM C = P1RL C 9 P1WD C B P2AM C K' P2BA C C O P2RL C F PBAS C C ): PBAS C C & PCON C C 6 PCON C C 4 PDMF PDMF & PLOC C 0 PLOC C +} PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 4. RUNT RUNT RUNT Rate RunN " " S/N% & SCAN 3 SMED
SMLt SMPL SPAC Afg SPAC & SPAC Afg SVER 4.0 SVER Sn SVER , , Scal @ Scan 3 TUBE B10 Tmpr < User phAR phCH & phDY big phQL c phTR phTR #; This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS Sy BufT & L Q CTID CTNM ! CTOw A CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA (= Pz DATA
(= Pz 5N DATA (= Pz DATA (= Pz B DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab a FVoc a FWO_ GATC Feat / / a GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE N LAST ~ ~ Q LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS & P1AM C = P1RL C 9 P1WD C B P2AM C K' P2BA C C O P2RL C F PBAS C C ): PBAS C C & PCON C C 6 PCON C C 4 PDMF PDMF & PLOC C 0 PLOC C +} PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 4. RUNT RUNT RUNT Rate RunN " " S/N% & SCAN 3 SMED
SMLt SMPL SPAC Afg SPAC & SPAC Afg SVER 4.0 SVER Sn SVER , , Scal @ Scan 3 TUBE B10 Tmpr < User phAR phCH & phDY big phQL c phTR phTR FB}}Cg/D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TNc:=?k>b7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nN {%t=s(>CZA$}[H#[zL(NApK^bZ]"Po1Cd}F<_`VWY+a:WkeC.%oh^5Q1V?|&@8je]i~mcR=J4WAU i8/gQoOriv4*un,T(k"T'ogLOE2(/(W,XD3J6EX! w+[_3z4r$>FN}ds&v_zy8oHO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX>V