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'BQxPNH2  "9T} h\F8 "    A?oshgB3  *4`r!Q'fh\\_12   -4!,BMNInVdGRN/)  2,,,III^^^{{{&&&999DDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR$ SSSKKVjj"""+++544==@EFLNNUVV^^^eeemmmtss{{z<<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e4114fd520d3477590226d69d9da8b49Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_19-52_21163130Seq1027010270_Lehman_K1357_SNPMEX_rbclrjmadera96-WellIndeterminate_sp_K1357KB_3730_POP7_BDTv3.mobSeqPop7zms}+Q{6a%UhhU9~#4?A;1$[3 Gdpm_OE@>4l"O/GId8 "9Tr1xa 5\?b|wg[WY[YK2_-hw+h1p\OE?;73.*&$$%&''%" $)-/.*'&'+18AL\s'l b V!)l>1Y&\!d wjrHJm~vhZM?, Hslv2-Nl Z?/&""#%$!  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J V b o z  ! , 8 B N Y e p }  # / < H S _ j w +6BNYeq| ".<HU`kx  +8COZfs&2>KVco{ ".;FR^kv*6@LXdp{ ".;GR^jw*7COYeq} &2>KWco| #0;GT_kw !,8DP^ju+7CO[gs*5BOZgt~(6   )",)./!" $:6::6::$46)4>1>>>>>>)//3'>>>>>/'>>>;, ';>>>>>>>>>>;6>>;>>>>>>>>>>.  0"!8.>>>>>>>;>>>>>>>;>>>>>>>;;>>>66>;>6>>>;>>>>>66>>>>>;>>>>>;;>>;1+" %.&8.6>>>>;>>>>>>>>>>>>;;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>8>>>>>>>>>>>>>>>>86>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>'' &'>86>>>>>>>>>>>>6>>>>>>>>>6464446444.)444000404:446):6/(+0)/466:66:::::4/)666646044*:1:0(2.2 32( #0!/2:::33(%2(2"%10#14.+*)$(    )",)./!" $:6::6::$46)4>1>>>>>>)//3'>>>>>/'>>>;, ';>>>>>>>>>>;6>>;>>>>>>>>>>.  0"!8.>>>>>>>;>>>>>>>;>>>>>>>;;>>>66>;>6>>>;>>>>>66>>>>>;>>>>>;;>>;1+" %.&8.6>>>>;>>>>>>>>>>>>;;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>8>>>>>>>>>>>>>>>>86>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>'' &'>86>>>>>>>>>>>>6>>>>>>>>>6464446444.)444000404:446):6/(+0)/466:66:::::4/)666646044*:1:0(2.2 32( #0!/2:::33(%2(2"%10#14.+*)$( .*-KL#P+K%N!3(\(N:\8,a=80/<aH[bJF'5#1+>,I#\)D#\#=98C9 `1`GGS7>X0C,K7 6 ^/%VD=P :LN^+/,B>S& V`<0Z]&L$? 9M%<CS4cHY2I< `QV2 `5\L!.XVUT,*5#R'5/(B4.96A*)3 ^FdQ8_/DPcVL %D0) #-?UNCa(+(Y7bcN'H":E0 \-7":A5U@5OX.,%I6!0Jc8FNRN<68[%AUGAADcB8F8 N^ % 6 KI! /N  bN_9 @K'0F1BD$S-M`/Q0B>MLK4 "#`FF@ EA B#cD5//J,797(05=T;(?K=DJDJUCYG;^C9X.?A]..'b/BS0V;%1CE)GR=`R&,LN8*S$= ".P31W#""`>9=[3UL"d+LKU "?MA](.C;;06! *39]PK;(Id+:>N=V2L^LW 76T*Q\Af_qxiNB'eODA* :e6)Z, }R @ lr ICoc= Wb9C9drZ4 ^nAXm  b: .oE O@\j^l~2aP Kj}6ORPZ[^ "K] dRVTvq$j{ER %yde x1-q .Y9 ,EA4(r{H#Y} m)CD+"C2` M1u7!?" E$u= QAC4}uoIQ8lE~DO9+NmU9{}#=}}cx,+SgW WOyCS =9\);SX\m}|z ?`+nX{lXZ tYG \ B Hvq## u7 +Jxa_fan_OVrSJSKR::6;:?-<15D5#,%-$&Sg"     \H                  %*  %  &   % "'*(Z#/--A0 )-$@#=#&B=)*&$"5BvW E5?")9648''H<aN#*#6/6//(=>!;/::RA-SFW%44/9;B?X+PLD=LIJ6e5T=A;BQ44lK4XOHI4IeXOsP99D499D9899 =pp{pppdpZp9D Uubpdds~2P},@&_8u=6X",|Op,_MZ1| DVl~8%~]kd,|5MoITld'p?8vp&>?JEpL%y+o,?E,xe:mp!* Z)pD1)r,G.:9Q  ,0xCt)Ucp/ 8\ox8F_B czp <<,>w,/*d tb&VVB"-fVLrSP8SICfK@\>F7 ;]PEw)/MHIs^t@ -= &(F# '$&"",4.$*G# k8\(zj1!* )$# #(!-$%"-%)0!#.5  );"H9Dl.$$r6dB( "!# !=($8+-#%+%$:A(+5+ 6# + *#:,-- "'*8&(6" ;!)-/++$%%+,! /$ # .&/& ((8!)'.23%)7F'#/.60O0&*-.N?>78089,94:&2J./2>$6B$!-_&Xd?;*#-)56/ &g"E:XJlE-++kAeRwqfm~4 #PZ:/=*xh8'D"#B G{*r1jRf>79-k/6xER?TI/}bD]a8_##X.V4?]*MVEv O% 8XGGAATGTGTGTTATTAGGATTTGATGATAATACCGACACACATAATATACTTCAGAACACGGCAACTGATACTCCCATAAAGGTGCCGCGCTTCGAATCACTTCTCCAATCCCCCTTAAAAAAGGATGAGCAAGATCTGCAATTGCCGGAGTATCATCTTCTTGTATATTGACTACTGGGTAGAACGGCCCTCCCAAGAATCAAGATCGATACTGAAGAAGAAGCCATGATATCGAGGATGGTGGTTGAGGAGGGTAACGAGGGAAAGGCCACGGAGGCTATTCCGGAGTTCCCCCTGGAGGAAGGGCCGAGAATGGGAAGGATTCATCTAGTGGAAGGGAAGGAAGATGAATCCGATTTCTGCGAGCGCTTTTAATAGGATAAATAAGTAATTTTGCCGCATAAACGGGCAACATTCCAATTTTTTATTATTGGGTTCTATGTGGGAGATATGAAATTTTCCCCTATTGTTGTTGGGGGGTAAGACGCTCCCCCGCTTCGGTAGATTTAGTATTTTCCGAGCAGCACCGGAGTAGAACATGAGTATAGCGTCGCCGTAGAGGAGAGTATAAAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 21:24:13 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSWBufT&LQCTIDCTNM !CTOwACTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA (QlDATA (Ql6@DATA (QlDATA (QlDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~ULIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS*P1AM@A?P1RL@<P1WD@EP2AM@NP2BA@@S?P2RL@J?PBAS@@,PBAS@@*PCON@@:PCON@@8?PDMFPDMF*PLOC@3PLOC@/?PSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%*SCAN3SMED SMLtSMPLSPACAcXSPAC*SPACAcXSVER4.0SVER VSVER,,Scal@Scan3TUBED9Tmpr<UserphARphCH*phDYbigphQLcphTRphTR:This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSWBufT&LQCTIDCTNM !CTOwACTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA (QlDATA (Ql6@DATA (QlDATA (QlDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabecFVoceRFWO_GATCFeat//e#GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~ULIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS*P1AM@A?P1RL@<P1WD@EP2AM@NP2BA@@S?P2RL@J?PBAS@@,PBAS@@*PCON@@:PCON@@8?PDMFPDMF*PLOC@3PLOC@/?PSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%*SCAN3SMED SMLtSMPLSPACAcXSPAC*SPACAcXSVER4.0SVER VSVER,,Scal@Scan3TUBED9Tmpr<UserphARphCH*phDYbigphQLcphTRphTR^pF?jA]RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+gO5+ nT~RW)9*=!2h`qq>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y,n/_g4<6BEPY $,OZ{bP{g,2caQ9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wT\3>>B]'p8Npd-(bcn23isFbotrV+5|=L*=B%E+bCB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>Vl&od3m&?j|D-'Kx3} 6hf!Y '(doz1)U`]yVom &uCgoz#OmmcfT`Q&azo_FLL:E3Sk-[qr[&X1EDE?]mnjH[$.}RZ#3|5O-U[d1DFW%j'kT'_C:Or4^7~s?K90lVSFsA bXX<,x'EEwiOfFWcDArh]|Ko5qS+SP7T]Tcr[&$&?_1OPh~jTn/])RpikE:b&b)>`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtAUDT||svB1PtB1PtBCTSWBufT&LQCTIDCTNM !CTOwACTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA (QlDATA (Ql6@DATA (QlDATA (QlDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabecFVoceRFWO_GATCFeat//e#GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~ULIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS*P1AM@A?P1RL@<P1WD@EP2AM@NP2BA@@S?P2RL@J?PBAS@@,PBAS@@*PCON@@:PCON@@8?PDMFPDMF*PLOC@3PLOC@/?PSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 4.RUNT RUNT RUNT Rate RunN""S/N%*SCAN3SMED SMLtSMPLSPACAcXSPAC*SPACAcXSVER4.0SVER VSVER,,Scal@Scan3TUBED9Tmpr<UserphARphCH*phDYbigphQLcphTRphTR