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S S S K K L j j j ""!+++444===EEENNNVV[^^deemmlsssz{z < < < < < < < < < < < < < < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5a607b57b20649d2abbd697c78abe0d6Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_18-22_2115 3130Seq 10269 10269_Lehman_K1357_SNPMEX_rbclf jmadera 96-Well Quercus_sp_K1357KB_3730_POP7_BDTv3.mobSeqPop7z "@\p~ M2Tv- Npl^ Z 5 h
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5 7 k } S 82, mB ] [ q -e"[ ` m| SP ? a X4 wJ q:> jGAAGAGGGAAATAAAGTGTCAGGAGCGGTGGTAAGATTTTATTATGCACCCAATTTACACCCTAACTCCAACCCCCAAATCTCCTTTAGAGTCCCTCCCACCGGGGAACTCCACCACCATGGCCCCCAAAAGGGGAAACCGAGGCCGCGGGTGCCTACTTAATCCTCTCCTGGGATTTTGACATATAGTTTGGCTTGTCCCCCAAACGGGTTTGGTTGGTTCCAAAGGAGGTGGCCCCCTAATGGGATGTGGGGCGGGGTAGAAAAACCAATTGTTTGCTTCGGGAGGTATCCCCTTAACCTCCTATTCAACCAGCCGCTCTAAAAACGTGGGTAGTTCCAAGGGGCGATCTAGTCTTGGTATTAAGGTCCTTCACGGAGGTGGTCATGTTGGAGTTGGATAAACATGATATTTATCTTAAAAAAAAAAAACACGTTTTTATTTAAAAATAAGAGGGGGGTTTTTTTTTAAAATCGGATCCCTGTCCCCCGGGTTAGACTCCCCAGTCTCCCCACTGGCGATATTCTCCCATCACAGCCTGGGCGTATACCACACAGAAGGATAAGGTCCGGGCGTAGAGTGTATATCCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-05-02 19:52:55 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS _w BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ) SL DATA
) SL 7 DATA ) SL > DATA ) SL ފ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ ] LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 1 P1AM O I' P1RL O D P1WD O M P2AM O W P2BA O O [ P2RL O Rc PBAS O O 4` PBAS O O 2 PCON O O B: PCON O O ? PDMF PDMF 1 PLOC O ;M PLOC O 6 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 4 RUNT , RUNT 3 Rate RunN " " S/N% 1 SCAN 3 SMED
SMLt SMPL u SPAC Aad SPAC 1 SPAC Aad SVER 4.0 SVER _l SVER , , n Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH 2 phDY big phQL c phTR phTR 0< This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS _w BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ) SL DATA
) SL 7 DATA ) SL > DATA ) SL ފ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab m FVoc m FWO_ GATC Feat / / m GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ ] LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 1 P1AM O I' P1RL O D P1WD O M P2AM O W P2BA O O [ P2RL O Rc PBAS O O 4` PBAS O O 2 PCON O O B: PCON O O ? PDMF PDMF 1 PLOC O ;M PLOC O 6 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 4 RUNT , RUNT 3 Rate RunN " " S/N% 1 SCAN 3 SMED
SMLt SMPL u SPAC Aad SPAC 1 SPAC Aad SVER 4.0 SVER _l SVER , , n Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH 2 phDY big phQL c phTR phTR V#\*x9*ofW)NK[>9`ht1_n8J{]t>fDb6b@*-D~=7?L+EK|T+1#4v*'bvxZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_?DNchv{"$U]`EzWkp$=RyS4*% `%c~]M:]A|7ZGM9V \+1w.@{Uae[&BTd.u?zcw~CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72WeG>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H%R7R@(l\[B!("n<[vegq?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4 DATA ) SL ފ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab m FVoc m FWO_ GATC Feat / / m GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ ] LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 1 P1AM O I' P1RL O D P1WD O M P2AM O W P2BA O O [ P2RL O Rc PBAS O O 4` PBAS O O 2 PCON O O B: PCON O O ? PDMF PDMF 1 PLOC O ;M PLOC O 6 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 4 RUNT , RUNT 3 Rate RunN " " S/N% 1 SCAN 3 SMED
SMLt SMPL u SPAC Aad SPAC 1 SPAC Aad SVER 4.0 SVER _l SVER , , n Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH 2 phDY big phQL c phTR phTR