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I B M 4 9 ^ X M = = ] A D = 9 4 C Z B 6 J 6 I = X W H > W = 3 Q 1 5 = F E H T ' 6 C 8 B D C S I 9 9 F 2 Q J = : H E 9 @ O B I W 3 > ? ^ ? E / E @ = 6 7 J 5 : 1 J . , D P = H G U F 7 W 4 D = + ? P 9 N E F \ < @ E D ^ C G Q R K C J @ M I ; S R = C M = G < E P ` I B : 5 3 2 m D ; ; 9 Q @ C 6 P < < U @ 6 4 P . I \ U X I G I @ / L J R G ? > D = B O G G = E D * D @ N I I D 1 < - ( L : H 2 0 a H 3 @ < C ] K D U E P f E E A 0 R N 3 = 4 < M = ; D G I H : / O > C ? D 7 - > 5 8 7 9 : T D L Q E Z L P F > W F C 0 L > E E 7 @ > H + ) 4 = 9 + 4 I ` K F J D W L 2 ? F T ; > C 5 R I ; A < Q A 2 G 5 = " ` U C H L J M F > B O O D M 8 = 9 4 @ 8 9 3 ! = E ^ N B K G : T : J B C : 8 O ; 1 ? L I Q H N * E T @ < M : c - J @ J 7 > 3 < 5 L Y = 0 A N / < C R M I ( A Z 7 F 4 8 > N : 7 ` H \ N 9 @ C - Q P H B K ; P = N ! : E e M 4 A C E N T J 9 M J K 1 B @ V F O ; @ O < ? X M D B T @ F F R 7 O A 4 A < M B : T 9 D . X F 8 @ , 9 R 0 6 J M Q H < N W - 9 5 E U L Y L H P P ] E H W D Q a C : ] U R ; < P M H 1 A L = B Q T X @ G I 3 M R 8 I I ? ] > < ; P B J X 3 H ; @ S 0 H 2 + Z N H F Y < V W T 1 O P : E A D K _ H / 9 O R D + C F P H E = U M 6 O S _ 3 E U N @ M B < S X ; ) A F 5 H H h 4 4 : Z K F @ < ] ] M I O E E : N 9 = B G ] 7 L ; Y K E ' : J 6 O ^ ^ B : U 5 = V A : J 5 . Q ] 5 V H P L V T T S U 9 N T L ^ 8 ? 1 X ) 4 3 B Q 5 ^ ] E J F H E I @ P Y Q L 3 O O O Q R @ 2 L R F B Q N ! C : Y 1 @ B X [ K N + C ; O > < > , L J X : 5 P 7 \ g 9 S * A A B N 1 : E I Q F = ; N O @ 5 f H ^ ? > @ > 3 R \ i J < ? R ? O O d k O I P E @ 2 ? 9 H I I ; G R @ B L = = e S J H : A 8 < D Y E I 9 @ P \ ? H Y T > : @ ; D 6 9 V F U Q D V b : ? : D N F F X - K ; S < B = P P S F a G I L B W ; ? A ? 5 6 S ` - Q 5 C P L V \ 9 j F X ` n A k > N ; F : ] D J K V ? 7 S > : ? 5 H G ; ; N B M M K I 1 5 T : = D J W S G L 3 J G 7 M P M F L X < ? : 0 J E E R A 7 m ] N Z D L I H ? Z 8 N P C < 6 B W 4 G ` > = E P ^ Y [ e T E 8 E D % C R 9 D N Z F G E D A E N 3 L B + U 3 6 E R S E 1 T s W 4 P ` ? s P = 9 P = @ _ ; L d H G > C D C , Q N H 1 M ? 0 5 J ; V G V K F A A = T V J ? Y 7 V 5 L L W ? C ! ] = J 9 G J A G ] K 4 R ? V I : \ U N : K 6 G X N 8 Y ? E U S V 6 4 T ^ M A F D W V I 3 = ; V ? c 9 C < . I [ M L J 1 M L K W W D C j D 7 / P W 5 < 6 F 6 . > K Z H ] Z > I Y D I a I < 9 ` K 5 F ] ` S > = ^ [ M M A C C ; ` E I B Z L V Z * = ] > O A E G 9 V 9 > I W ; F J K I 9 H A ! I H ] ; g J _ @ B N G E U f 8 ^ E : B H S Y O J I U Q @ V b X D \ C D K H F C Z M Q J k [ ` E N H E ? Q = F : L > @ E T Q E F _ k b T ; [ F b ^ X * I M \ U I O / R D B E I Z 8 1 + > W Y A 9 N b S H O T T P ^ : L S H D 9 X K H S Q L J \ H P A Q ; S S N ? N @ X = E W L T P M R ] . J N N [ E + \ M \ O C f D U O C \ E U * I > * Y O D K ; F ] 4 = Z G W H = O L E O = J 8 ; Y ` ^ ( X A B O @ ? a A F ] : K 6 ? P N M \ 4 b I ? > E ] N ? P S E Q S 4 @ A [ J 2 L < P T M I _ E Y A ] \ D F I a X d Q R X < : [ R 8 = F = D T [ T M K G \ F K F A ` Q + A B G I ^ E A Q 9 Y H V ? H Y ] L Z Y Z Y R B N F M e S T ? K f R 3 @ G P P A F P U i P Y I A b F 7 _ Q I O U M O O M X O Y O B M Y T q T : G N B U , Y K Q ; \ j F A : A 6 > M G G ? 9 C D e G S Y 4 Z M G T [ ? U @ C Z @ S D M D ; N I ? C e X U 8 ? N = Z V X N J M F W T R _ V M E \ P G b R 8 I I d O Q J \ H O 8 : D ^ S L O ; H \ 0 < L ^ H K 3 _ J C R U X 8 G R S @ Q U a W I j V D L D ] @ R S K K = < X Z B T P D N R P G Q L N ] U > D ^ ] f ` R S = V H P A J ] W F C U + 9 : > U F = 4 L N ? V a G J G 5 = ` @ C B Z : 7 < X Q G G _ E C V W 3 P B N K Y Z V G W L Q K C U M C L ; M ? \ J Q F U S e G X a E d E K < 0 ` ^ Z J > - Y P ` m J Y Q H H C G : E S _ I b [ ? F f k G Y I U Q P [ E 1 G J I R M W g a e d ? < N P T b D I : W M e P g H Q J T J G I K N d > H V e U S A K e S A B Y : , Q 5 ? H H R [ @ X 5 S > P T R S T 8 Q b C F H B H C ` c L [ D L _ 5 O R : - 9 G F K X F ] H ? @ V C T 7 C 6 J 6 F K S I = @ a B < O 5 D @ H H R L M 5 W a M V P H P P R @ \ G W Z Y d W K : U 3 O g K M F D Q R R A N P ` ] ? Y 8 X U G J E ] O Z 8 @ [ = A I D b A N : G P @ 9 J V [ d S ` 6 0 Y > P p Q T K C Q > @ P M K \ I c M E T H < a > G K S _ b W = V [ B Q G I e M X T T D X [ B 3 ] V = J R J ] Q L E P P \ 5 Q L S ? 9 P V 7 F H = R 7 P = @ \ N > M b T B D W L > F Q W H M U N A P K F B B S ] i N < Q C G P S E L U G H E ] E W M L X V S V Q L Q M @ S J c ` ; K ? Z 5 X _ G J & N T = Q W \ ] T I X D X < V M h O ; L X W ] f 7 C E 9 M ? P 6 > ^ ]
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 981ffefa6624424d992694db556f31bcSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_10-14_2110 3130Seq 10268 10268_Lehman_K1356_SNPMEX_rbclr jmadera 96-Well Lobelia_sp_K1356KB_3730_POP7_BDTv3.mobSeqPop7z 1 4 8 > F O V Z \ ] ^ ` b f n { !>UbcYKBDTr 8M[cebYM=- pX@/)0AS^\K-
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KB 1.4.1.82017-05-02 12:02:37 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS i BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA # F DATA
# F 2 DATA # F x DATA # F DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FWO_ GATC GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE I LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM
E P1RL ? P1WD K P2AM
W P2BA ] P2RL Q PBAS PBAS ' PCON PCON 9 PDMF t PDMF PLOC 3 PLOC - PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER ^ SVER , , Scal @ Scan 3 TUBE D9 Tmpr < User phAR phCH " phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS i BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA # F DATA
# F 2 DATA # F x DATA # F DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE I LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM
E P1RL ? P1WD K P2AM
W P2BA ] P2RL Q PBAS PBAS ' PCON PCON 9 PDMF t PDMF PLOC 3 PLOC - PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER ^ SVER , , Scal @ Scan 3 TUBE D9 Tmpr < User phAR phCH " phDY big phQL c phTR phTR Mr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;N }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V.#LyN6EC2Ow/p(f/R,QTb^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0oW~!Te4;,: TNc:=?k>b".\7x+45A*KPp$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VD*p;I:/wEkfptir"8|oTq}A|1D@<7>{AXG9{$}_uEpj coSVf9bbC4Setzu^h0|5Q1V??}@8jaFsA bFX97z?&qN^Fu*t&:_Gr9#(eaK|`Q@#=,`Pf)NKo-jE(_y"X_tXL ViY2]2rj&3d}7&I]N@c'v+gK >xPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPz%I!|spKa3gDkS${N|X-N&W-dOsW&wzl`O_mz AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt AUDT p p , B1Pt B1Pt BCTS i BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA # F DATA
# F 2 DATA # F x DATA # F DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE I LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM
E P1RL ? P1WD K P2AM
W P2BA ] P2RL Q PBAS PBAS ' PCON PCON 9 PDMF t PDMF PLOC 3 PLOC - PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER ^ SVER , , Scal @ Scan 3 TUBE D9 Tmpr < User phAR phCH " phDY big phQL c phTR phTR