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v o o o k O V m ` X a [ N V W p \ F Z Y B D V p t _ h Y L Z > \ ^ H X d ` C n O Q W V 8 R N M 7 S E H V L L U T > > ] b H k @ C F c Y D j L C 2 [ 6 ; a \ B ; _ 3 L E 8 X ] < 8 @ C S X N C M 9 O c F E e R Q ? T > B ] P 1 J : > H Q C W - S U Y U C \ D G ? c N C B I Q d a 9 > A F B ? J 9 D P g 6 Y = V T F R B < _ B O _ @ ? J Z B G N G D H : & O D M L = H R B < 3 B K = 1 < Z Q M N V L P C I _ ? 4 O K X b ^ E e @ 9 8 > P @ B J _ E S < Z P 6 ] Y c : C 3 = I N + K 8 J V ? g O 5 E X Q Q [ J . 2 L @ J 1 ` G B E H P T H K A T I M H ; D F G X F H a 0 V @ 8 : F H 7 O B 5 5 B N O 5 D B I H J 8 V F K : H C > ] > 4 ! 5 H D # 6 O W H < F Q D C K ? 5 N ) F a A F D P I > A @ F R : F O L ^ Q E F D ; O N 8 / 4 5 = / < ; / 6 F < P G P R 7 ? B / > : = O # 7 2 + 5 < / N & 9 ? 6 E E @ E ? J ; F N Q Q B V 1 B . ( A A E V O F ; < F Q 6 N N D 8 6 F ; = R 8 . ! A F 0 ! F 9 < - J 2 D K ; A 8 7 K > 9 H ; B M L 8 O @ H Q C D : J & < B @ : : : E : > ? W J + ; L B = % 7 B - < [ Y F G ) D E I 6 C > : I % B : ) 7 U 2 2 D = N 5 B @ @ : 1 . G F ? @ H B 1 C ? E L I 0 O A ? C 3 e . D C 9 U F B 9 U G 5 I I S E 8 E / 2 H I > N J 8 J 2 D _ @ 3 L C K Q @ Y N < E B 6 ) l + K Q C 0 < @ G A / B I M 4 @ - > M S N 7 G C H @ M ' 6 C ? N . N W H G ? M O K 1 9 D ) 2 ? & B " : 1 T < A 4 D @ : M < 0 X R P D W D D O M @ F N 3 1 , A Y L A M ? F A G H P 8 + D 9 E Q P > G J 5 9 T 7 Y S D A D = K O O B + G G = 8 > C : 6 K R K < A > S ; \ > 3 ? P ? Q < @ B M ? J 7 S 5 C 8 U 4 @ Q ; E > @ 3 G W @ * 0 S ? A 9 & < U B F ? > 5 ; + O F 9 6 4 X A M J S > = A P O E & : ? G 4 K 1 S G B G 9 < 6 J Q + ; O > U 3 @ A 0 ] L / M % / I S G 4 @ C L 4 U L F ? T 2 8 8 I A J G 9 B j O @ 1 N * N 6 / 9 M J X = , 9 , 8 W R 9 Y i 2 ; = D G F R Y [ > b @ ; 3 ; T 9 < 0 ? M @ H = ; 2 E K . D F ) ? C H 7 D 7 9 7 D 6 6 K F ; X R U I M 7 6 = Q D A J - F 0 7 K B G / ( ? K O T 4 > C N A 8 \ > Z Q Y T : $ X I [ 0 I 2 K < B 7 ; D _ V F ! K , Q ; < G F W T V 2 + = D H : H O L N E 8 5 [ P P L N G I ! 0 C ( b G & L 4 G - X J A C F N > V [ 7 O W M = " D 5 L B ` W B I 5 6 K ( F 8 7 : G T 2 D < O : ; : 8 8 H : [ , U F Q O ; M Q Y 3 K N C = V ? ! _ H K > D 7 L ; D A G < E b B J > ? 8 A B H ? C 7 O 7 0 B H R J k E G H E 7 7 A = < X Z < , 4 9 H C A N 3 P + C F E 3 N 4 ; S K J C J 1 . B = 4 < T 4 H 7 6 M 1 ? . 6 A I D > @ G L J O / 6 7 E G @ , 5 ; E : 6 6 : Y B F < ^ E F @ K G @ @ L H 7 E 3 2 H K B @ G 0 5 9 9 O S N B N J 1 8 = G 1 B ? : > X K N < 5 S @ I 2 P i < \ M K < J U 5 D > F ? > D U 0 = Y H ^ Q B A V < L 0 F N ? W V 5 M H 8 8 Q b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h A N B = S D 8 F < U + I = : D @ D @ . ; K I < @ 7 E 8 ? K V ? J 7 7 I Q A ; : Z O M 3 G : A G 4 > 3 S Q P J D : < D P Q 8 0 7 A A J A T K M < S O ? Q T O @ 5 , G \ ? A w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e C 5 9 7 ? Q ] H E % M K 5 O I G A V > J 4 I O J [ 3 = : > A 6 1 N L @ & Q 4 ? A B R J 1 L B : R a = > @ @ 8 A 6 < ] H L 4 1 0 J F K > - 7 9 C 6 J M 2 3 I B 6 5 0 7 E E G F E @ R J 9 T 7 1 6 4 V X < ? H A H J I N D @ R @ 4 U G F I 7 0 H 7 6 @ Z F B < 5 5 V \ A = 9 < 4 R G 3 Q / Y 6 0 ; S L Y 1 ; 8 E Q A 4 L B 7 [ U T J [ 8 K G 6 < 1 J M 0 O 0 J F S ? 5 I S 4 C @ $ G = J K S @ E J m F a O 8 M S P : E ? D < L / R K J a I D D L ; L = L P = G L T C K M U J 2 H D B G G : J 5 < G > @ 9 D F E N W P T 9 9 [ H W P X E 3 ; T K C : N B 9 G H S = \ > A : V L J Q A ? F 3 R F J Q F ] 0 S ( A F J 7 U E V @ B 0 > 4 A P J I ? N @ > M G S 4 P 8 . D ; G Q < B X P N E T ; ] N ] Q n O X = D G M C V 9 H T @ J @ P 7 7 G U S [ C / L K D 5 B S V A A ; [ X X G F X E G @ A K X E 7 I f B Z > W 0 T ] U M = M O G I J R D T ; 1 C J O A ? S O G Y , T E K K 8 S , N J R G > P : ? @ ] ; N < F M L T K 9 F L X > ^ K Q - E ^ J ? > Z G N 2 ? J R O 9 \ / 2 O H N \ . R E J 1 P H Z U C 6 C O O L L = J K E K , 7 E G F F G 2 9 ; K K > D Z M < F B S G 9 U F F 6 A Y b S c 3 J O S 3 W = 6 A K E ? = M \ M 6 V G W D ? J > G Z 7 8 I W = : H E K ) A N ; c H Q @ J 3 C L M F 5 J 7 A R V S G H ^ 7 @ P A F 0 > J ` 7 M B H & 1 G N B 0 M 9 D ^ V 8 G U W < B O 8 Z < M T $ D K F R 4 > G H V : K Q M 6 S G K G < C K E : f M F = - \ N Z G T \ N > f H J P Z J H B B ; X c 8 7 3 F d O ? > V = M Q ; G 4 T Q > O 8 L N U @ C B X 7 L W 9 F < T [ I C ; F - M b 1 H P > Z X N Y V I F J E 5 5 L M V G P A D a ' B Z P j P S c U H U > ^ - V < R C E U O M < ; S L > N S Y > 7 P ? P Z S P > K F J H P V > D 1 K Z e R \ . T E ] C a D T A , B E ^ S L X O U E N O U @ 9 J J H Q [ P 4 ? P R Q > K A 3 R g B a J P = L B : P O < O W G P \ U 8 O : J ? C 0 N N > < U N Z X C ] F X D F B S L 7 p V D B 7 > X ^ O L K A a V I 8 J K @ Q e W V A C P : T Q O B K 7 X G L : E 4 : S f R 8 A H M O 9 G E Q I i F 2 P C M B P Z _ > L < ? ] H b > C ; W U P D C Z 7 4 ? ` J T F G T > E D D F K ; + f Z I O L D V K L M @ P B < J A < G J \ U G L f X I H ; > Q O O R I L L M X . X W P S ] < \ C T V C ' ; B J C ? T T S 9 7 ] Z R G E E S G \ F T K G B V S 6 5 d Y B X : h c K 9 D M X , D C A W a C ? ? < O G Q 9 F U F J W B 6 P S 3 V 1 : N 8 N P Q K ] \ Y ? G 3 O H [ K b f N O < N V V ; J 0 T R 8 f H : V X 4 Y K J _ O C G I O V A E O M Y = B < < 6 3 _ 6 K P < H E K I ; I . N N [ E h S ` P T E O B @ M J T x C B T A I 3 M M H B R Q N j G L J M I Q N 5 d G M ` g L : N 8 G ? f @ \ N ; N F H B N c M T A N @ T T = G S X E B C G 7 R 9 H P @ : ^ H e ] P V P U T M E U 2 L > [ 7 ` X O _ C D T M S C S B > > G J L Q V ] Y E A L P M 9 K = L T k G N J > D T J B Q N U C ? C F 1 V E M I F A J A X O I W E _ V G O R [ ; I I ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bc964e707c1c46df983686979cd52c91Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_10-14_2110 3130Seq 10268 10268_Lehman_K1356_SNPMEX_rbclr jmadera 96-Well Indeterminate_sp_K1356KB_3730_POP7_BDTv3.mobSeqPop7z 0Jg"Qz!]-v`%^l e
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KB 1.4.1.82017-05-02 12:01:44 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS TU BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA '[ N DATA
'[ N ;P DATA '[ N DATA '[ N ؼ DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FWO_ GATC GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ R LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS 'z P1AM E >_ P1RL E 9 P1WD E B P2AM E K P2BA E E P P2RL E Gs PBAS E E ) PBAS E E ' PCON E E 7 PCON E E 5K PDMF z PDMF ' PLOC E 0 PLOC E ,7 PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% 'r SCAN 3 SMED
SMLt SMPL c SPAC Ad_ SPAC ' SPAC Ad_ SVER 4.0 SVER TJ SVER , , Scal @ Scan 3 TUBE D2 Tmpr < User phAR phCH ' phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS TU BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA '[ N DATA
'[ N ;P DATA '[ N DATA '[ N ؼ DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab b FVoc b FWO_ GATC Feat / / bp GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ R LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS 'z P1AM E >_ P1RL E 9 P1WD E B P2AM E K P2BA E E P P2RL E Gs PBAS E E ) PBAS E E ' PCON E E 7 PCON E E 5K PDMF z PDMF ' PLOC E 0 PLOC E ,7 PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% 'r SCAN 3 SMED
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'[ N ;P DATA '[ N DATA '[ N ؼ DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab b FVoc b FWO_ GATC Feat / / bp GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ R LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS 'z P1AM E >_ P1RL E 9 P1WD E B P2AM E K P2BA E E P P2RL E Gs PBAS E E ) PBAS E E ' PCON E E 7 PCON E E 5K PDMF z PDMF ' PLOC E 0 PLOC E ,7 PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% 'r SCAN 3 SMED
SMLt SMPL c SPAC Ad_ SPAC ' SPAC Ad_ SVER 4.0 SVER TJ SVER , , Scal @ Scan 3 TUBE D2 Tmpr < User phAR phCH ' phDY big phQL c phTR phTR