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D O H I T ) N > V H i W S F c U Q G Y C N N n < [ ] k Z Y Q 7 B 8 Q Q T @ a O ] d c M Y N X Q E N Y ] F : 9 T R C ^ 4 J M 9 M Q K \ D ] D V N > A 4 L P M Z , Q Q > Z : = = > N 0 8 I B P P = = C G ) > @ b ; H K V 5 L X T ? < 9 8 F @ 8 U 0 = L O J ; W D I F D M @ T G Q U N Y G A W ] W ; ] c L O [ B K H [ H S C I M = ^ M Q J J Q R K N h e M ] U W Y b c P R J R O C h S X W ` r m j b e W S F h Y \ B Q J Q a ` ` c C I ] 7 P O c Y a G ` X h l _ s k | V a P T N a N f c U T Y J a j k l \ c Y q q \ v z o Z e t N l q k p u e u f w a d a m g [ k S f k z v p ` ^ i Z Z s V Y \ Q C Q 8 M ^ c v j u z \ n l s } ] c U z f D V L a o N c Q H y v W f t l e m w j m u h } z r f J o Z f m [ t Z [ _ u [ P e R b _ j p v e [ ^ n M j E X ` b J [ c Z N Y ^ p T Z c e w \ _ f E ] \ n ] Q \ [ N D Q : M ; W R Z K 9 + + I ? A 1 > @ C N & K ? : = 9 ; 5 2 D C @ 8 R > X D I + @ = L 2 ? 7 G 6 M < E Q R 1 3 5 > Z G N @ R H 0 J ] I b D 6 L N 8 P K K O O R \ r Z X k i a D R Y O \ O N A C P \ < e G K N Y E T J M r L W Y Y ^ k N [ f Y c D ` 4 h h P T S b j X h ^ I \ h b d e Y H ? X s ^ Q b b N f c C b _ ] i T O 5 V G ^ ` R V : A M e Q H ? K Y e ; C 3 / S M C \ I = M B [ O S 8 D G ` H K U ] : @ N G D ; 9 ? D ? Z N D O T S K P J X S E V 5 I D U L C X ; : K J = ` O O \ 1 U T V W U F M : K ] : H V M K U q N N Z Q = B 7 7 _ h W h ^ O R f d ` X Z U A W \ f H a g N _ X P h c | ] p c W p i n | o i W j g c s X f m K w k X w j S y l n s m t u _ r t | Y V ~ d k | X m q e ^ P U r i q g k p k O X Y Q _ ` x V l ` _ o i ^ V l c g k ` p { j o ] q u s k w i c r X o y e z j z s ` x s e t p q n t _ ^ ^ p L h l V j t e o ^ g b f c w c a M e b _ i r f r q o r [ s k b j b S b c h Z M X _ V P c m b n a Y n z Y ^ n h i j f \ U W b V M K ] r Y W z q w h c p y s _ | { p a k w g q _ q Y ` e l j [ M c w p z j [ ` v l = h L ^ ] m t k U d R c \ _ p ] e i l p s m s ~ ` a d x h T ` b Q j j f c q r k q v g q } p V w r f x } f u t w p ~ e m u t ~ } w k l } w o } | _ x u e o z Y W \ d Q Z n f x f i q k d ~ ^ o c v u z } l x ~ e z w h i c q ` V X Q i S e L Z f = : a U G T B 6 X _ M S R A S X Z @ M S D G C : G : I X < @ 6 B R B V N D A H 8 = F 3 K L E ? L / = P I J D - 2 G B D I ; 9 N : ? P W Q H = D A L : E ? 8 5 @ + C B K ; T 0 E E ; = R # N ; N 4 0 < B 3 - F B 8 J 7 ? 4 W N 7 D D A 7 @ R / D P B B < H F ; 9 D ; B 7 T , G D 7 J 3 D B N 5 T G 5 : < , 8 @ = 4 3 1 - ; @ 3 ; & K G K D < 0 < 4 : / . H F ? / = Q ? 9 7 7 7 @ ; @ G N P E / 4 4 ? J / . , + M K E A = = M F M b 4 ; % * 6 A - > @ H @ ? E @ B - = A 3 > V G : K 2 ' L A 7 * < 5 H a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e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` @ F K B D K G ? . 8 N 4 c I L = 9 5 J E 8 F @ E B X 8 @ . # < B \ > < ? q * 7 0 6 H 2 > ) 9 5 # 7 : 4 H N A = F J @ G 7 < I A N / K ; # ) K B > 9 - < A % @ = 1 W A " . A 2 D c 0 ? @ B 5 E D A < 7 7 , ; 2 = ? @ > 7 = K B : O K I 9 + E > @ 3 B G D Q 7 1 B U 8 ? / 1 6 % ; D ) ; F A Q < B * C N ? < = > A < A / A T / 9 W @ 0 F 4 K ? 7 7 6 ` 4 - 9 7 ? < > > A , A : 6 M @ < D P F S 5 - P L : 4 U 6 + = + 6 : = = 7 @ 2 7 H 3 1 5 : / < M H = = O - @ / . = / J ) 7 S > e < = E H 3 ? [ ) P 4 6 C : ? 8 & 6 P 6 7 : 9 ? = R = , 4 & 1 A + = W 7 & < . ( A ( = 2 H < 4 5 L 4 2 + D D W 0 A 3 : = D 6 > @ K G H Q * N , 9 4 / G I A = ? A J E 2 C ; @ + # ) = @ Y B % J > * 6 7 L 1 B C 4 3 3 J 6 J = / 8 E , C < > P N M @ 7 I 5 E E / > G 8 V J > > > L 6 > 7 : 6 - > L 6 @ * 2 < : 2 ; J 0 ? ' < $ R H I S J B 6 3 @ > 8 U 2 2 a F H E > > B D @ N B M H G = 1 K Y ; F N 0 F : D : 7 J : A S ' C ? A = F > G E J A D ; & > A < H O = ' C . 0 6 . M 7 = ? R F < K 0 Q = C 4 ; < C K = C 2 ? C 6 P Q A 2 4 9 @ 0 ? I U K B : 4 0 3 7 @ ] 9 - ; 7 h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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4c6847cb9a4747afbe4f740caedcd55fSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_10-14_2110 3130Seq 10268 10268_Lehman_K1356_SNPMEX_rbclr jmadera 96-Well Callceolaria_sp_K1356KB_3730_POP7_BDTv3.mobSeqPop7z ",4?Pj
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RX144 \pWW UF "9 f H 5Bu XmI_f Ox | m.+w L/$-'/JX)CGAAAGCAATAAAATGTTCATCACGTAGGCAATAACTTATCTTTGCCAAATTTGCCTCACAACACAGAACCCCAAACCCCCCTTTTAGGTAGCGCCCCATCGAGCAAACCACCTCCATGGCCCCCCCGGGGGGGCCACCCCCCAGGCCTCACAACAACCCCCAACCCTTCCCTAGCCTATAGACCTTGCTCTCCGGGTCCTCCTCCCTTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-05-02 12:02:07 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS V BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA #
F DATA
#
F 2 DATA #
F x DATA #
F DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FWO_ GATC GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE % LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM
b P1RL P1WD P2AM T P2BA P2RL PBAS PBAS $ PCON
PCON
PDMF y PDMF PLOC p PLOC PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER K SVER , , Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS V BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA #
F DATA
#
F 2 DATA #
F x DATA #
F DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab $ FVoc $ FWO_ GATC Feat . . $q GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE % LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM
b P1RL P1WD P2AM T P2BA P2RL PBAS PBAS $ PCON
PCON
PDMF y PDMF PLOC p PLOC PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER K SVER , , Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR jk:/R",q;,`h?X??-|D>jH}3^ %_ef2rgHGygLVFX^smgnWuYvQm{p,nhT}{Uw2yy>T3pb}0Zz>z5Cw1ubkv:cGave>u\nLTH:#6=ANp[F5TY/5`agv9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&xrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>RbS4}(Qbq`>?0g]^GnCz:"]ddc}"Qz)l]iOVe`A #pJEa#;{U,HB*@Yy-4$&+VFl@i2+`'Mvf0iSo!:#`(DdUfo0.l%+`n Hh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob{\`d\}5L6Q^Zew.k.d}uNf1&vM\#YTEl?lT6qLg\zN}tRw3KF ?%VaO]!:Bwfmk=;A{%iTK(>V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{ow]|8:39}!@^sW/&:#Q1A0OHb- 7@o*.AQ>U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d# RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER K SVER , , Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR