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S H O j e > F Q w R T \ d @ [ H S _ k V S V p O d Z O X Y [ f ^ q { o _ l ~ o m ` k j S n t p s l n L E ] N j q n r _ e ` c b ] Y T k f e P i t S ^ i ? L H , 2 Q e P Z T U U [ ; U I Y R b Y H M [ E s \ @ a b r h > \ V R H ` c h F d B N @ I I M S D D V m [ f o d [ Q F d ~ m S h e ] T i \ ` j c c L ~ ] [ _ j ^ ` x l o e k c W q f o P h \ Y J M d W n P a R Q ] E y u ` I [ q \ ] d f w M i b ^ h u s ] O s V q e R a S h T [ _ X [ F y ` w \ a W f I i ` g o D k g P S P I K i G g V n X i y X ] \ m Z ` T d [ g \ t Y Z W G U Z W G m e a ` x W L ` v b \ d ` x r a ] q t m g W U m k ` j d r r R c b ^ m G @ J = ^ [ j K e h a b D J P F s S p a ` r z r } v u u h | } { r y | { v w r m q o X d j \ ~ _ Z G ^ W > J 7 L H 0 J ] U ` L E : - L 6 S T I Q 9 4 N = R 1 Q @ ] c C 5 J I 7 G > D Z K 2 D D 9 J 0 9 / % D S 3 < B : I 7 @ M H + H 9 < B ? X = D ? N B P ; B E @ 2 6 X @ 8 D J W ; @ ; R 8 = L M N K ? A = L P ? / > = U L T 2 * * ? 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H U G O < 7 7 7 9 I D ) 9 # : 9 < C 4 N N O ? H ; M 7 L ^ 5 , ; F N % E C > D ? 8 - F I F * G 6 A 9 F [ 7 3 P @ [ 4 ? 8 < : J ? 6 4 7 > 1 X < : H F 4 @ @ ( I < D D D / 1 ! H < J 1 = A [ V J L : : = ( 0 7 0 , C / > . , = 9 5 4 / K & F . J W ; 6 G X a 3 E = ] , H M ; 5 A D * K G I & . . J F 3 - ? M + M 9 @ 8 J - ( 9 ? 4 6 6 L 9 E : 3 G C : A N 8 O + N , C E + 0 W I 6 ' 8 5 5 # P ) 2 A 9 H 6 . 5 5 @ " % A 9 N @ 1 C 1 H 3 + % - @ 2 : I 7 _ O - H : < . I ; , : < : B 7 + 6 : 6 / ; @ j 5 6 < L G 4 : C ; F 6 C E = 8 [ @ = 8 0 A ) C J Z U K < : = ' - 7 9 ' ( + # B 8 C J 4 T ' e = F ! & 8 ( E * 2 @ < @ \ 8 . 0 < ' G H ; 6 9 L 9 ; A . M > # J P : 1 7 ) 5 ? ? 3 5 L E > @ & > E ' 5 3 C N 0 : 6 6 f A 3 O = = 2 8 O 8 / A L ( J H C 6 2 8 ? 5 B 1 8 * 6 A C ? 5 = > 6 > I ? G 1 E ? R . . H 0 0 C : T 0 + C C + ' 8 3 5 G M K G . & 5 0 = , 6 F 5 A 3 F / 5 A < # 6 E ; \ > A O 7 ; @ ? > 9 2 . > A 5 B M E d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b 5 6 / G > ) 3 ' S 5 # - 0 8 ` $ 5 Q % < R 5 = 6 6 D C S ; H < 9 I 5 8 E J * / . - F > : , A < F D A F L N ; 3 6 ) 1 8 4 = @ \ 6 L K 6 ? : O 6 ? = F J / 4 C > ? W ? > P 3 + > 7 B > * S M 8 B 8 G ( J L / 9 F F F X J C Q O K ' 2 & K < A 5 9 < ? 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G M < M G D N 0 D I ; = 5 B M V R : H P * 5 0 O C = = B D < E O D S W > B @ D j I H . E L ? C , G ; 0 6 M 7 W J b K G H 9 J L ; V & : T U c I H * D H B O I @ D H @ 6 F 8 R T C T S 1 , P B J 0 F : I 8 M 9 H F K U , [ A ^ C : N < A $ K F R 8 P > ; @ N P R N M 4 Y 9 A Q H M C 5 : Q R J + 6 ] 9 > O C , F < V 4 : @ 8 O H 7 H = 7 L J 8 D ^ F 3 C J I 4 I : 7 O D D S \ G 8 X L @ C S 9 Q L : N > C M : ) M R # 1 T N J < > - C V = H ; 5 ) A L ; . @ M ^ ; I + ( > B i \ & @ I @ 3 ? K > + 7 > I I 1 5 K g W J N > X ; H C Q P R A I K K I J C U L P : E G ? > F 3 R / . N < Z @ J ; ) I H = I K 8 ^ E H 5 D Q H : J = C 7 H a Q Q 3 H = ( C = R D 2 + 5 = ? K 8 4 [ Q _ = I + C , 0 G D . @ < R F I O ; I > E E = + F C K N Q K C C = K c c U < . 8 S 4 < L J D / 6 S I @ B e O = . 0 + P 5 : Z E D D > T L : > 5 X Y 5 B ; - M A F ; Q F B 8 9 : H @ X [ C A ? X G ^ H > B 8 G a D : R C @ K F L H T L _ Y N U F 6 I P B R : > ? : E T G W H I B Z G = M A N > V N ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6e57638f2ec7402f9c695afaabeba1ffSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-05-02_10-14_2110 3130Seq 10268 10268_Lehman_K1356_SNPMEX_rbclr jmadera 96-Well Anoda_cristata_K1356KB_3730_POP7_BDTv3.mobSeqPop7z 3 : D Q a n t s o r *C]s "$ 5JWZSF7./>Y}s`E%t]MEDHLMKF?941.-,+*'"
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KB 1.4.1.82017-05-02 12:01:48 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA " E DATA
" E 2 DATA " E x DATA " E DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FWO_ GATC GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM ~ P1RL ~ P1WD ~
P2AM ~ P2BA ~ ~
P2RL ~ PBAS ~ ~ 9 PBAS ~ ~ PCON ~ ~ - PCON ~ ~ PDMF x PDMF PLOC ~ PLOC ~ PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A1 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ȯ APrV 1 APrX } } ȸ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID S CTNM Y CTOw y CTTL | CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 8 DATA DATA DATA " E DATA
" E 2 DATA " E x DATA " E DCHT DSam DySN ] Dye# DyeN h DyeN m DyeN r DyeN w DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT
EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! < HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! ȅ LNTD 2 LsrP a MCHN MODF Ȧ MODL 3730 NAVG NLNE ` NOIS P1AM ~ P1RL ~ P1WD ~
P2AM ~ P2BA ~ ~
P2RL ~ PBAS ~ ~ 9 PBAS ~ ~ PCON ~ ~ - PCON ~ ~ PDMF x PDMF PLOC ~ PLOC ~ PSZE ` PTYP PXLB RGNm K RMXV 1 RMdN 5 RMdV 1 RMdX > RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A1 Tmpr < User phAR phCH phDY big phQL c phTR phTR -"3oAZKC[p3}$r&kUV'4Mu*}c+-O)C@5&J06THKoS]/~8R&}[>FESt%e]:aH<6.tJdQeK~jca1n6*X!afT7+|4C9|z*yEwiO*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/6?|$lQ%e+Bw9#MBz3)-I5d3g9kw6T-(WxdUo`($'tV&MWW|;WL.]_93Qq[%w%:T?NWFikas1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-=DaJ2`>nX$t!TQqdE.>X$!$wb0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~yHAj}="WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$X?;9y8hI0.wv|$@^]?\Hif3qE`HoG83:8\_pfXpi6m&} 9E^h;Zig\c!nY5Ikys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
56 RUNT ! Rate 0 RunN " " ) S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A1 Tmpr < User phAR phCH phDY big phQL c phTR phTR