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'$/, 7%  '3>>FK=J3%  31L0+LD=&   FEOV\HWHIDB<+ +%      5>1,1=E8; KanxtW[Y##5     d,,,III^^g{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR~>SS KKKjjj!!!+++444=<<EEEMMMUUU]]]dddllnsryyze<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 55b8fc0a08c849d5ab27e3809fcac641Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_15-03_21133130Seq1026710267_Lehman_K1356_SNPMEX_rbclfjmadera96-WellMuntingia_calabura_K1356KB_3730_POP7_BDTv3.mobSeqPop7z!6Sz3e(y`h2P 1  i  U = r | t m b L +  l J -   e L A @ < ' @el lLYj0  >n K'f  ""/O(2\^ s%}$~7NIzCs?*]~r@)OL uZOWp#Lp7`XJ[vT!zn]$ r.F0v!3}N:H20xbTJ?3("!&-253/*%    <o oiqJA)JrC!+C`KEp}oO)oCcL;,   0g/oru+\0   ?m9ORE- )CSVH)dC0%   4yUccGY\vN%]#+ SCi* YFL bs%       "%',5F\uoQ6#/Z'V]q4 T&oeehlqu{{jVA,".<Nd|iN3 [ Oo2o< =  "$"!(6FSZZWW^k{  '6AFC9+?K,^ m#ih"v;   #tX< C<  .'Og\O NwD    $# $((%  Ga] l|*]]  ecRYQ $)(#     E(8[\d'/7@HOUXYXVUW\dmtwvsqsx||tidx/2Z{B/2:<6,$#/FhV87\ 3id )4<<5(ExA`Br3 DJQ 8GZeZ|Gl@f;P 0 GO4_,:7  D5f-Y9=9kY< .t>S:  27LmA[ T#D%s#(a'    M4}J9\WjD 6:_Q~M(Q,s N  !    mQDF]|'   ;da|}. 2;d*A&IrF;     ;o }V   >|7H08JN#8 /W    "#"0Ee6SS3g.    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A K(Z20.N>!P'N%, +(=(:82<P-,$9._N2% 6C4M!1B a 2 =JA|'Ot:lwt flRvl)O!3;2g",CmKc OpP14rtBB) 6_$|%oCGH6F>C C;W~EpB J/[  Z7wI>>]2'b9 M}"oA;U\W?,`VW737F=5c?of,pQ/ckozgd@m|oze|+lH%7{ha3; .ZY_F [[zZa8PiU5=v}.<`MN7'9 'H$lcb{*eh/K+}VYlZTSyOUfm+Qw1#f18bdW='Bv:'A`tIP0 P H57 =D C>v%AwWr:Cy`ClyqoIW<=Z;/AD:*7<&&/i##,mr59$;#A...  $7""&        ,   " d?@E7;*$9 #( !!' 3)7 3%)  (! ' :? '58 '!$4 %  +E;- /68Z51 +*<]- %%3$D%%W &E,2"/"""""S" 5--[H&  %L*---:'06v--+++G0L,L.N^24(0Zk*7JWX66NNNUU%NNN66CBB=4 g4?NMGYBNN~6N3BBRBpB:BB+*MA@NNA`HX%3U BB5BBN66BN6666NUNPNNNxfp1dtujZeI2ds%p#p9nI !N6N2$n+td&$U6mxi~pYG,zq<OLA<rpW;uLS p+Ii@",Jp v;F8\C ,d"! 2`n,Tpt[p8qdgMYdIup?88  "Qfdb,T[poEpQUNn),0J(#>ZTp3 g p 9l259`<5p@xEc`ZK.M?AvLtpA2zqB;T&VXM`_(EC< `,31.e,)5$"1\ R 5]:j./=.*(4,'" #, 6!85"!1%>B)- aI(K C2S'&,I32,,-f=$+mI,W Ni@8ug+i}F(857|6^U(]_Y;=6Q8I.-TQK? Y l&!=%6|9|CYI*K]dg; &5J&6%(-32k<h(Z4d$VAL:3 N/@%>k<7@RL7R3=QF$mQTF0*/Bk N.%qDfM?*1eD\Y?_(.P6(?zc]7&4l' $B72:CIn,Y3/u{2sGhv-"iSx249d[\} _QW-86K&5OW11~F^PkT6e1GoH8,X<p?=i`&L8oN(5<a]h-$y@P)@,P![=#aLx4E+%Q7)&$9Z})QkDP*V$f'<MTtzFS-LRswlaU7sUIqk_GAAGAGTGGTTTTATACACGAAGTTGTGATTACAGGACTTACCCACATAGGTGTTCATCCCAACTCCCCAACGGAGGCGCCCCTAAAAACTCCACACTTCTAATCACTTCTCGAATCCCCCCTTAAAAAGGTTTCACAATTTTCTCTAGAGCCGATGGATCCTCTTATTATTCAAACGCTTAAGTCCCCAAAGACCCACCCAAGATACATTATCTACACGGAAAGAGAAAAGGTAACCTCCAGGCCCCACCTCGATGGGGGTAACGGGGTATGCCCCATAGCTAGTTTTTGATTCTTACCCCCCGAGGCCCGGGACGTTTGGGTAACAACGCCTTTAGACCCAGTCAAAGAGGTTAAAACCTAGATCCACTCACTAGTCCAAAAGGCGACAATAGTATTTGAATCTCCTTCGGGCCCTAGATTCTAAGGGTTTCTAAGGTCTATTTGTGGTTGGTTCGATTGAATGTTCCACCATTCTTGTTCTGAGGATCTACATACCCCCTTTTGTTTGGTCCGGCTCTCCTAGTGCCAATCCTGGAGAGAAAAATATGGAAGTGTTCCCTGTAGGGGTTAGAATACACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 16:34:51 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSYBufT"D1CTIDCTNM CTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6DATA (QDATA (QږDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~XLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS,P1AMGCP1RLG?P1WDGHP2AMGQ9P2BAGGUP2RLGLPBASGG/PBASGG,PCONGG<PCONGG:sPDMFPDMF,PLOCG5PLOCG1WPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 3RUNT !*RUNT RUNT  .Rate RunN""S/N%,SCAN3SMED SMLtSMPLuSPACAb+SPAC,SPACAb+SVER4.0SVER YSVER,,nScal@Scan3TUBEE9Tmpr<UserphARphCH,phDYbigphQLcphTRphTR&-This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSYBufT"D1CTIDCTNM CTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6DATA (QDATA (QږDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabgFVocgFWO_GATCFeat//gGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~XLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS,P1AMGCP1RLG?P1WDGHP2AMGQ9P2BAGGUP2RLGLPBASGG/PBASGG,PCONGG<PCONGG:sPDMFPDMF,PLOCG5PLOCG1WPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 3RUNT !*RUNT RUNT  .Rate RunN""S/N%,SCAN3SMED SMLtSMPLuSPACAb+SPAC,SPACAb+SVER4.0SVER YSVER,,nScal@Scan3TUBEE9Tmpr<UserphARphCH,phDYbigphQLcphTRphTRRz0L/kbE!0z^n3HVQQEO2astcZ~~VGC2lB)lW+Uf066}ZC__cIBM}.z zFef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`P|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTveurDmCq?`DQ`+}{Uplql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W@Vk1$#=4ZbZ4yM=VrTH&zqrua*b iq*#7rO/adu{>(Jt6qZ><}P?[NB@vulc*{w@p;#(":?S`,j.uFTcM#yKA#2rBA++mqIlEN&w|1d$\Lw7[*3C'B (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flaO8JB6E$} {r*i``Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9} P,hz9_"#kjFO-U[ds=:I1On(`"]/r;H>L<|U0YPUIZr+);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;