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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR;SSSJKKijj   !!(**333<<<EDDMMMUUU]]\dddlkkrrrzyy~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a866ba7ada574ddcae89d9755ce82913Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-05-02_12-02_21113130Seq1026510265_Lehman_K1355_SNPMEX_rbclfjmadera96-WellDesmodium_sp_K1355KB_3730_POP7_BDTv3.mobSeqPop7z+Ko-\:h >z3a%E[fe[M@4*!  /F]s@YcZ@v9jf W 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Bvh4FzT+ /WvH% ?p zO=Ju$<4V(       4j)xO@Q{(L#      :kR* 8n6/ a5    #Do^4/a*S^HS'   @n ^HQt U/ +O|uH%+O|]4%@]xy_B(&ItX1   !:]c?#  @i\<% 8Y~`IF[aYlyN- %Djb?$  )Fl}U2 5ZeB% ?jhRObdZeiH/     *Ilc?!3U{lJ.   )CdyW9!!8VweTR`}qM0 $BgpYQZqnL0 ,KqhD(    -F_v~jR9#!>cnSFHXqtT5  !5Ok{\>&   ,BYlwxo`PC>EXt^<#  (AambevsR6" ">cfF-     1KhfK3       *<Tn|fVQZosS7$   2Jexg\Y_m{`E.)?XrvaM;,!     .Gb}~iS?,  +:HU`gif]P?-    #6Md{u[B,  #7Ng|zeM6# "8SooT;'    %8M`ox{uiWB-  2LlqU@3+'$"    %gA4A#/A\A#PHKB.bcpKB_3730_POP7_BDTv3.mobtermTCGAGTAATGGGCTCAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCTGAAGAAGCAGGTGCTGCGGTAGCTGCGGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTACGGCATCGAACCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTCGGTAATGTATTTGGGTTCAAGGCACTGCGCGCCCTACGTCTAGAGGATTTGCGAATCCCTAATGCTTATATTAAAACTTTTCAAGGTCCACCTCATTGCATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGCCGTCCTCTATTAGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAGAATTACGGTAGAGCAGTTTACGAATGTCTTCGTGGTGGACTTGATTTTACCACATCGAGTAATGGGCTCAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCTGAAGAAGCAGGTGCTGCGGTAGCTGCGGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTACGGCATCGAACCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTCGGTAATGTATTTGGGTTCAAGGCACTGCGCGCCCTACGTCTAGAGGATTTGCGAATCCCTAATGCTTATATTAAAACTTTTCAAGGTCCACCTCATTGCATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGCCGTCCTCTATTAGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAGAATTACGGTAGAGCAGTTTACGAATGTCTTCGTGGTGGACTTGATTTTACCACA !.?ISdm| ,8ERbjz  .9DR]hs "-8DMYfr+7BNZep} %0:FR^ly ,7BMYdq~(4AOZdo| !-<GQ]hu   - : F R ] g s ~    & 0 < H T ` n x    + 8 C N Z g r    $ / : F Q \ h t     & 3 ? 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DQ0VDc (#;1)5"6D5EJ-$T".3U*UL@.8"!TN0]Z O;\= I$8- _=$DPK)>W%[D9U<"cBR-I)X3"c X_()McPU*,:@!?2#Y L XBD AM8@.+4CS U$QC U#/0,A/8*>1Y Sc4**G.T'.NJT>Yb5CYP&0=E)QAV  4 ZL57A ()3)?E/LES%96B!8)?c$+VY 8>BF ^NA FT ; 0A\aU#J?#?&E-"$  eI8)47i7GE[AH(,!t+ni:Fn &s_US%bqSmG8v~%( `|(i<*vNJ)?Fq< $zLz?v^o8&+~"4O#2Y(nIxI&Y5&:|uq:yY_B3jf<2 2OQHu0l1@fA3J=4;g A1- h1" !X'g_;7;TZ#?H.gYoPU`V)B[mYrLi@bz-obpN(fl~-63L:wBu}-0sVArGGqdYR-Ye^g%%rAT @m3 iU?}(( JPGG@n TqD=!X eB * y> #.L4(?  ]JVO H p ()vI-*n%T^`laWX_^PRgRI?D07R6N:MD; 2'/+0J1/8.&)#   8  dF              /       !& #/4  $#% "%"#!8(&:) !.+ ,$". " &****. #3 :(-$)0$,<#:"##-DA>%95V199L,34H3?'$999 3/CFQ5IC:<64,@^QCTFCC#*=7GKD=^HOZ9AEOMMMDJ<KiCPPMKhwVLfMZoHjNLTMerYZhQXw:u_gs^Scc_Ja`W{SzPCvQaqqy_XPPMPPPPMOaMM8ppp,h2pu`B 8,,I8E pppdn Cyd }DCCwU.'_pdDgd8Z@^k2]dAZ#y+AjpiEP1{  Y;Gdupw2&dV|pd<:k p,pp"#5~pdD!PqVZppcHdeg,&f+89,zT8,sFH%%{tp*38p 88 <7y2X% &!KD:laL"%0Il[>! 4#^|6.!#`sdD S\{_h_K78,3"j !I+&!), @e/&U1# ~bfny:4"B0*5&"#"" '5(2 -+%Gh("uy8""'#2 -4%!4!""!=$&! ":%& " #%# : &.*#B;.!*,#(%+!,") %9!&$2',)#'6!7&"*#%$32 59={!$%(.8'%@7.,,(!9,=3! ?<$.647$K#&5I7-!#$'90!#;" #2A['C:50))82([<XNg|[DFP%3X$3>NQK79 .N1> /JWaKN.=O;\-kmPI/To5KJUG@)R=9U+~8d/}9B $+eAAAGAGGTGTTTGCACAGGTTGTGATTATAGTATTTACCCACATAGGGTTCATACGAATACGGCAACGGAGGCGCCCCTAAAGTTTTCATGCTTCGCGGTAAATCTGGATTCCCCCCTGCAAAAGGAGGAAGATGTGCCTACAGATCCTCAGTAACCTCCTAACGGATGAAGACCAATGTGTAGAGCGGTCCTCCCAAGAAAACTTATCGACACTGAAGGAGAATCTACCATATAGGGGATCCTTGTCGAGGAGGGTAACGAGATAATGAGGGAGGAGAACATTCCCGAGATCCCCGCGGAGGAAGGGGTGATAACCATAAAAATACCTTCAGTGGCAATTAAATAGGTTAGGATTAAAGTCCGCGCTTTCTTTCTAGAGAATAGGAGAGGATTTCCCACGCATATAATGGCAAACCACGATGGGTTTATTCGTTTATACGAGGAGAGAGATATTTAAAGCACCAATATCGTTGTGGTGGGGGAAGAAACACCCCCCCTAATGTGGCTTTGATATTCGCTGAGTAACACACGAGAGCACAACAAGGGAATGCGCCCTGTACAGGTGAAAATAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-05-02 13:32:57 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSUBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (Q6DATA (Q65DATA (Q6 DATA (Q6@DCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~TqLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS)~P1AM@@1P1RL@;P1WD@DP2AM@MP2BA@@R1P2RL@I1PBAS@@+PBAS@@)PCON@@9qPCON@@71PDMF~PDMF)PLOC@2PLOC@.1PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT   RUNT  RUNT  RUNT   Rate RunN""S/N%)vSCAN3SMED SMLtSMPLkSPACAbSPAC)SPACAbSVER4.0SVER USVER,,fScal@Scan3TUBEG7Tmpr<UserphARphCH)phDYbigphQLcphTRphTR6This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSUBufT!B)CTIDCTNM CTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (Q6DATA (Q65DATA (Q6 DATA (Q6@DCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabdUFVocdDFWO_GATCFeat//dGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~TqLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS)~P1AM@@1P1RL@;P1WD@DP2AM@MP2BA@@R1P2RL@I1PBAS@@+PBAS@@)PCON@@9qPCON@@71PDMF~PDMF)PLOC@2PLOC@.1PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT   RUNT  RUNT  RUNT   Rate RunN""S/N%)vSCAN3SMED SMLtSMPLkSPACAbSPAC)SPACAbSVER4.0SVER USVER,,fScal@Scan3TUBEG7Tmpr<UserphARphCH)phDYbigphQLcphTRphTRk&zt Mi3rs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!IkWgt/eqi1jfAFs:)-WS|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL