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"  *                                 *                                                                    ! !                                                                                                                                                <,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR&Wdgiiihhggeda`^\ZWUSQNKIFC@>:8630.+```VVVzzz),,888DDCOOOZZYdddnnnxxw                                                                      <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 20f1755099574230b6640ac3356ebe1bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_03-27_05503130Seq1227112271_Costion_K1358_matK_Rjmadera96-Well K1358_F6_RKB_3730_POP7_BDTv3.mobSeqPop7zA8p0}p y I Gy\&5n |!"$"%'*K,/91358:/ACOEGIKMOkQXSHU3W XZ8[e\<\]]!],]5]>]9]\[ZYX VUSQOMJGDA?7=O;::,9P8P7,543{2@1/.--+,,L+*)&$"t 6UrV8J} [4;xb3q^>> . 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Z@PAxAGAKB.bcpKB_3730_POP7_BDTv3.mobtermCGCCCGCCCCGCCGGTCCCCGTACCCTGTAGGATTTGTATTGTCATTTACCGATGGCAATGCGAAATAATTAAACATATCTTCGACCCTTTCTATTTATGGCCTTGACCATGAACCTTAGCAAATACAGGTATTCAAGATACCAAAATAATAACTAATTTTGCTTATTTTTTACCTTACTATTTTTTTGCTGTTCATATAAGTGGAATTTTTTGATCCTTTATTAACGTCACCACGCCCGCCCCGCCGGTCCCCGTACCCTGTAGGATTTGTATTGTCATTTACCGATGGCAATGCGAAATAATTAAACATATCTTCGACCCTTTCTATTTATGGCCTTGACCATGAACCTTAGCAAATACAGGTATTCAAGATACCAAAATAATAACTAATTTTGCTTATTTTTTACCTTACTATTTTTTTGCTGTTCATATAAGTGGAATTTTTTGATCCTTTATTAACGTCACCA#%IRdq):DNXdr,6IVfr "/>NTeu/8EQ]fs~  +5BOZjv!3<GQ\eq|'/;EOXdnx&0:FP[fny    % / ; D N Y b o w   ! . 6 A M V ` i w #%IRdq):DNXdr,6IVfr "/>NTeu/8EQ]fs~  +5BOZjv!3<GQ\eq|'/;EOXdnx&0:FP[fny    % / ; D N Y b o w   ! . 6 A M V ` i w                           !   ,  &            6      "                            !   ,  &            6      "  F,7?J? ?%%K',$W1,^-'&@Y,WQ/3W,R!?2_c(c8O7C8%553b)-JG9?&'S.LXb5X7b# O:KSD7![MI!*+LS7<[X,E>%^c/+2I'##8-TC81P+)-&ZZ7O/IRA6(!c)F#<X@,?[D#H>7 <->%Y6#= :E36X/?39*:1AY3\!%r#D4>+'m"iS R+R ;_hfT*?W]/0lI&@)6* vop `| 4~thv,y*n"gHZ :Wj%8asiFy.fvPr3d:>_G`/  [B#H*nI{A<3IH=)mGP^z3)W&vMVY.pR)f~ H?[ZQ@2%J:(`Z,"^ ,3#)q1H] _  ( E:VE#dzp58ppO1 f6vts(SpAX\2[gtAjCl;#F %H80r)F<c,58BtwrR# ,:T>SUptEM85t{-'gddA {rS4&g.$C%u#Y-h3r#S, ZMCYS!t 0 e,G:gy{fEsBnV~p#1[RgY[f`*<%1.#&&%'b/]ivH3?UH's*cq*>.]M~B(If";iAOFQd_<u,s,V^+{btGAAAAAAATAATAAAAATTAAATATACAACACCAGGCACACTATCCCCTATACCATTTGCCGCCTCACCGATTCGCGTAGGCTACGAACGCTGTAAACCCCTGAATGAGCCCCTTAAACATCTTCTTGTCATCGTTTTGCCATCCTCATGGGCAATTAAAGAACGTCGCCCACTTCCCTCTAACCCCCTTACCCTTATATCCATTTTGGAACCTTCTGGAATCCTCATAACATCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 04:57:57 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtkASPtkB1PtjB1PtjBCTSMBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #G$DATA #G$+DATA #G$rDATA #G$DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS3P1AM P1RLP1WD ^P2AMP2BAP2RL2PBASPPBASfPCONPCONPDMF{PDMFJPLOCPLOC:PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9 RUNT 2RUNT 9Rate RunN""S/N%+SCAN3SMED SMLtSMPL pSPACMSPACCSPACMSVER4.0SVER BSVER,,nScal@Scan3TUBEF6Tmpr<UserphARphCHaphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtkASPtkB1PtjB1PtjBCTSMBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #G$DATA #G$+DATA #G$rDATA #G$DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab"FVoc"FWO_GATCFeat.."hGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS3P1AM P1RLP1WD ^P2AMP2BAP2RL2PBASPPBASfPCONPCONPDMF{PDMFJPLOCPLOC:PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9 RUNT 2RUNT 9Rate RunN""S/N%+SCAN3SMED SMLtSMPL pSPACMSPACCSPACMSVER4.0SVER BSVER,,nScal@Scan3TUBEF6Tmpr<UserphARphCHaphDYbigphQLcphTRphTR--)ob2=Y?Z8Z~]`m2W@V{%e_dQ<,N-AT$L8zX!3xn/+ O]rM701`jFB.VVQ8il6jJ}B~xGH$@iQ{nW)\lZhs| nG\|8 f&hPV)W)R6nL,rg`g6 ?63_`EzWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCO`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q I`X/13U`l${\Q`O96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+F4$T9tR3==QC0^,!Sk2%vAfIq}ubsCnHmVNPn2uhv]qoU)m Pl: ,uk&q6X+9mMyTAFUTT3T&^%FyPC,_,qz7A*k|_LhvYZ&UIg#p&rS'8$w?DQsgLY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d ClG>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H>R74@if\?;!(Zn<%v}Vq?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? 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