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"  %'& )      "  $   %                                                   !%              !                                                      ,,@II^^^{{{&&&9//DDDNNNaaavv      ***333>>GRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 18;=<<<;;98864320.,*(%#!   ]]]SSSvvv   ###///::9EEEPOOZZYdccmmmwvv              !!!"""####$$$%%%&&&&''''((())))****++++,,,,----....////0000111112222333334444455555666667777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7532a0b4ebdc472e9366b0fff1eb4c3eSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-20_09-08_05303130Seq1224812248_Costion_K1357_matK_Fjmadera96-WellK1357_A8KB_3730_POP7_BDTv3.mobSeqPop7z*[!6DX}6mU+:DVx;Yp\1 sAW*P%Ehm[PSd!6AA6$lDyjgmuvlV6yiP/)1-!  0GZdcWC+1e<wiF1g1<>4f8|.cJ<8<FR[^ZPA2(&-=Tk|xrj^O=)wpw"{p s+c @,* f90fu]D/!   k[ZdtxlaXRLGB<72,&  #Dl-6.oN8./;SyS  DU(_C/$"%+158<BM]otjc`bivg? #6J_p|tbL4 7_&-) lPCGYqoU=/0En%\zzjC *MmmP5%"-A[vX+"("sov*OcdQ. 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G S _ k w  ,5COZlu%1=IT`ny #-8DLXhq} ,5@O[hu*6BMZer|'3>KXbnz $0?IT_my (6GQ`hw               "       "+   ")1"""                    &                                             "                     "       "+   ")1"""                    &                                             "      W J_'&Y503TbT_Z8AICD" !NZc&B=!OY/AY]?3M?)__9P59 Y7L3") T<5&9#\FQd$,(U4MZ 0.QV$ ?c6%/ 1:KB!Z) [UFDWOG /YV\G3K5\<IK 88*+ T>XL5:B1O\8].NaPKME@G:O9 )]^X4J N& ) (A%V$+L(C^E\FKR\9Db^8RA,%\"2 Z/1LI T*+P.S9]=A_7/,,*_'N=A`T2b)@*FOET *&7!8] @; <,#'F \JI[ '?8dYEO& =/._`TU^ J"K?=37X1+ ["?$W76=6 /NC CN-S!"DI22H\TYS)HS,B2(%/] )^G .A2#ZM?@ PQ?A@M v k2Fb!\>C]<LR= ^ }P9xc AR}Y @=Gk7YfM?knW/%Ge`jy0yX5Q 8]ch 8b=j 1C }ivB 7 $q|2)o~;T;#AVY,VY'!6 ' |6~gOkB8)J.*$7Scu}/!o+ 6=o\\AH m0:#|`3~!TuH\8 h}`oL/9{aS8hg>6/[M<|N{5#P(G y0G !/}1 Dk?%xQR~SiaIA3Pl4C4{$KylCBYf ec$wZ3@$B3/TbG% 4.:1EN3<.0453`4h*(71:)Zm")#:')5&"I]1.!:a"C5 E.4 ; +=F!/7 9  X%43v7 4: .= {;&~-9loPFCaYx+g.B05m(e=V@OMf(/AZxTLNpe9q.Qh4k,X 8lMj?Yle3_io 8cCd}p!Jj6}aQ?!rSPFl0Q mJVrFBo [E {8,O8)+O9-Ql*?8Hlu@88m\\!=9vX#CGgA((p|?6Pd2DJ x I9_#GYiM):mF+g|Ywi#4.UD8O v  c II 9"Q5F{v/4;RoUdD 6w|9mgZ*9N|i[ t>FHsk){.b. ,{B,7Yj'~TJvie:~C!8nT=jN\l:#(nM }6BQ2} haOOsIl~+ !vaS`Pex_ D_V]|O:&% F|@B IuN"`B4 gN*,Ln`Eh{= [nZRb<' j "%]Lla)c?+{+zz&^)]SuWL]e8WdX-~:v &FDxT+ Q%pGAGTTCTTTTCCTTGGATCTGGCAAAACCTCATGACGACTGGCGGTGTGCATTATGGGGACTGCCATGGGGGCAACTATAGGAGGGTGACGGATTGGTAGGGAAGGGCGCCTACACGAGGTGGCCTCCGGAGTGTGTGGACCTCGGGGGTGAAAAGACGAGAATCAGCCCGGCCCGTCAGCCGATGGCCGCTTGGCATACATTCTAACAATTAGACCTAGTAAAATCCTCACAGACTTACTCGTTATAGAAAGCGTGAACCCACAACTTAGAAGTCTAGAAGCGCTTAATACGAGAGTTATGTTAACCAAAAATTAACCGGTACTACTTGCGATCGCTGCGTGATCCTTTAGTCGCGACCTGTTCCCAATCTCCTAGCCGCCCCAGAAAAAGTGGAGACAGGACGCTCCCACAACCGCCATGACAGAAAAACTTTTCTTGGTTTTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-20 10:56:52 -04:00AEPt3AEPt3APFN tAPXV2APrN ȧAPrV1APrX}}ȰARTNASPFASPtyASPtyB1PtxB1PtxBCTSCBufT |CTIDKCTNMQCTOwlCTTL tCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 6DATA DATA DATA -[}DATA -[HwDATA -[qDATA -[kDCHTDSamDySN UDye#DyeN`DyeNeDyeNjDyeNoDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~}LIMS!!}LNTD2LsrPaMCHNMODF ȞMODL3730NAVGNLNE`NOIS\mP1AM|n P1RL|jP1WD|qP2AM|xP2BA{P2RL|uPBAS^^PBAS\PCONhPCONgPDMF]PDMF\PLOC|cPLOC|`PSZE`PTYPtPXLBRGNmCRMXV1RMdN -RMdV1RMdX6RPrNRPrV1RUND RUND RUND RUND RUNT  'RUNT  7+RUNT  . RUNT  5 Rate (RunN""!S/N%\eSCAN3SMED SMLtSMPL TSPACAR\0SPAC\}SPACAR\0SVER4.0SVER 8SVER,,ǾScal@Scan3TUBEA8Tmpr<UserphARphCH\phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN tAPXV2APrN ȧAPrV1APrX}}ȰARTNASPFASPtyASPtyB1PtxB1PtxBCTSCBufT |CTIDKCTNMQCTOwlCTTL tCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 6DATA DATA DATA -[}DATA -[HwDATA -[qDATA -[kDCHTDSamDySN UDye#DyeN`DyeNeDyeNjDyeNoDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..^GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~}LIMS!!}LNTD2LsrPaMCHNMODF ȞMODL3730NAVGNLNE`NOIS\mP1AM|n P1RL|jP1WD|qP2AM|xP2BA{P2RL|uPBAS^^PBAS\PCONhPCONgPDMF]PDMF\PLOC|cPLOC|`PSZE`PTYPtPXLBRGNmCRMXV1RMdN -RMdV1RMdX6RPrNRPrV1RUND RUND RUND RUND RUNT  'RUNT  7+RUNT  . RUNT  5 Rate (RunN""!S/N%\eSCAN3SMED SMLtSMPL TSPACAR\0SPAC\}SPACAR\0SVER4.0SVER 8SVER,,ǾScal@Scan3TUBEA8Tmpr<UserphARphCH\phDYbigphQLcphTRphTRV#\*x9*eofW)NK[>9`ht1_n8J{]t>fDb6b@*-D~=7?L+EK|T+1#4v*'bvxZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_?DNchv{"$U]`EzWkp$=RyS4*% `%c~]M:]A|7ZGM9V \+1w.@{Uae[&BTd.u?zcw~CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72We`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`pCCWk>0&StM4R[JOHQ8,[p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQn `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gX